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Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation

Vascular dementia (VaD) is characterized by a time-dependent memory deficit and essentially combined with evidence of neuroinflammation. Thus, polyphenol-rich natural plants, which possess anti-inflammatory properties, have received much scientific attention. This study investigated whether Perilla...

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Autores principales: Kang, Hyun-Bae, Kim, Shin-Hye, Uhm, Sun-Ho, Kim, Do-Kyung, Lee, Nam-Seob, Jeong, Young-Gil, Sung, Nak-Yun, Kim, Dong-Sub, Han, In-Jun, Yoo, Young-Choon, Han, Seung-Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929086/
https://www.ncbi.nlm.nih.gov/pubmed/35723398
http://dx.doi.org/10.3390/cimb44010019
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author Kang, Hyun-Bae
Kim, Shin-Hye
Uhm, Sun-Ho
Kim, Do-Kyung
Lee, Nam-Seob
Jeong, Young-Gil
Sung, Nak-Yun
Kim, Dong-Sub
Han, In-Jun
Yoo, Young-Choon
Han, Seung-Yun
author_facet Kang, Hyun-Bae
Kim, Shin-Hye
Uhm, Sun-Ho
Kim, Do-Kyung
Lee, Nam-Seob
Jeong, Young-Gil
Sung, Nak-Yun
Kim, Dong-Sub
Han, In-Jun
Yoo, Young-Choon
Han, Seung-Yun
author_sort Kang, Hyun-Bae
collection PubMed
description Vascular dementia (VaD) is characterized by a time-dependent memory deficit and essentially combined with evidence of neuroinflammation. Thus, polyphenol-rich natural plants, which possess anti-inflammatory properties, have received much scientific attention. This study investigated whether Perilla frutescens leaf extract (PFL) exerts therapeutic efficacy against VaD. Sprague Dawley rats were divided into five groups: SO, sham-operated and vehicle treatment; OP, operated and vehicle treatment; PFL-L, operated and low-dose (30 mg/kg) PFL treatment; PFL-M, operated and medium-dose (60 mg/kg) PFL treatment; and PFL-H, operated and high-dose (90 mg/kg) PFL treatment. Two-vessel occlusion and hypovolemia (2VO/H) were employed as a surgical model of VaD, and PFL was given orally perioperatively for 23 days. The rats underwent the Y-maze, Barnes maze, and passive avoidance tests and their brains were subjected to histologic studies. The OP group showed VaD-associated memory deficits, hippocampal neuronal death, and microglial activation; however, the PFL-treated groups showed significant attenuations in all of the above parameters. Using lipopolysaccharide (LPS)-stimulated BV-2 cells, a murine microglial cell line, we measured PFL-mediated changes on the production of nitric oxide (NO), TNF-α, and IL-6, and the activities of their upstream MAP kinases (MAPKs)/NFκB/inducible NO synthase (iNOS). The LPS-induced upregulations of NO, TNF-α, and IL-6 production and MAPKs/NFκB/iNOS activities were globally and significantly reversed by 12-h pretreatment of PFL. This suggests that PFL can counteract VaD-associated structural and functional deterioration through the attenuation of neuroinflammation.
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spelling pubmed-89290862022-06-04 Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation Kang, Hyun-Bae Kim, Shin-Hye Uhm, Sun-Ho Kim, Do-Kyung Lee, Nam-Seob Jeong, Young-Gil Sung, Nak-Yun Kim, Dong-Sub Han, In-Jun Yoo, Young-Choon Han, Seung-Yun Curr Issues Mol Biol Article Vascular dementia (VaD) is characterized by a time-dependent memory deficit and essentially combined with evidence of neuroinflammation. Thus, polyphenol-rich natural plants, which possess anti-inflammatory properties, have received much scientific attention. This study investigated whether Perilla frutescens leaf extract (PFL) exerts therapeutic efficacy against VaD. Sprague Dawley rats were divided into five groups: SO, sham-operated and vehicle treatment; OP, operated and vehicle treatment; PFL-L, operated and low-dose (30 mg/kg) PFL treatment; PFL-M, operated and medium-dose (60 mg/kg) PFL treatment; and PFL-H, operated and high-dose (90 mg/kg) PFL treatment. Two-vessel occlusion and hypovolemia (2VO/H) were employed as a surgical model of VaD, and PFL was given orally perioperatively for 23 days. The rats underwent the Y-maze, Barnes maze, and passive avoidance tests and their brains were subjected to histologic studies. The OP group showed VaD-associated memory deficits, hippocampal neuronal death, and microglial activation; however, the PFL-treated groups showed significant attenuations in all of the above parameters. Using lipopolysaccharide (LPS)-stimulated BV-2 cells, a murine microglial cell line, we measured PFL-mediated changes on the production of nitric oxide (NO), TNF-α, and IL-6, and the activities of their upstream MAP kinases (MAPKs)/NFκB/inducible NO synthase (iNOS). The LPS-induced upregulations of NO, TNF-α, and IL-6 production and MAPKs/NFκB/iNOS activities were globally and significantly reversed by 12-h pretreatment of PFL. This suggests that PFL can counteract VaD-associated structural and functional deterioration through the attenuation of neuroinflammation. MDPI 2022-01-08 /pmc/articles/PMC8929086/ /pubmed/35723398 http://dx.doi.org/10.3390/cimb44010019 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kang, Hyun-Bae
Kim, Shin-Hye
Uhm, Sun-Ho
Kim, Do-Kyung
Lee, Nam-Seob
Jeong, Young-Gil
Sung, Nak-Yun
Kim, Dong-Sub
Han, In-Jun
Yoo, Young-Choon
Han, Seung-Yun
Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation
title Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation
title_full Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation
title_fullStr Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation
title_full_unstemmed Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation
title_short Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation
title_sort perilla frutescens leaf extract attenuates vascular dementia-associated memory deficits, neuronal damages, and microglial activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929086/
https://www.ncbi.nlm.nih.gov/pubmed/35723398
http://dx.doi.org/10.3390/cimb44010019
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