Cargando…
Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients
Patients with cardiovascular disease (CVD) and periodontitis (PT) show shared risk factors as result of the altered molecular mechanisms associated with pathological conditions. The aim of our study was to evaluate if the plasma biomarkers associated with endothelial dysfunction may also be related...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929118/ https://www.ncbi.nlm.nih.gov/pubmed/34287264 http://dx.doi.org/10.3390/cimb43020051 |
_version_ | 1784670790086557696 |
---|---|
author | Ferlazzo, Nadia Currò, Monica Isola, Gaetano Maggio, Silvia Bertuccio, Maria Paola Trovato-Salinaro, Angela Matarese, Giovanni Alibrandi, Angela Caccamo, Daniela Ientile, Riccardo |
author_facet | Ferlazzo, Nadia Currò, Monica Isola, Gaetano Maggio, Silvia Bertuccio, Maria Paola Trovato-Salinaro, Angela Matarese, Giovanni Alibrandi, Angela Caccamo, Daniela Ientile, Riccardo |
author_sort | Ferlazzo, Nadia |
collection | PubMed |
description | Patients with cardiovascular disease (CVD) and periodontitis (PT) show shared risk factors as result of the altered molecular mechanisms associated with pathological conditions. The aim of our study was to evaluate if the plasma biomarkers associated with endothelial dysfunction may also be related to alterations in the inflammatory status in peripheral blood mononuclear cells (PBMC). Patients with PT, coronary heart disease (CHD), or both diseases as well as controls were enrolled. Plasma levels of coenzyme Q10 (CoQ10), 3-nitrotyrosine (NT), and asymmetric dimethylarginine (ADMA) were assessed using HPLC. mRNA levels of caspase-1 (CASP1), NLR family pyrin domain containing 3 (NLRP3), and tumor necrosis factor-α (TNF-α) in PBMC from the recruited subjects were quantified using real-time PCR. Patients with PT + CHD showed lower CoQ10 plasma levels and increased concentrations of NT in comparison to healthy subjects. ADMA levels were higher in CHD and PT + CHD patients compared to controls. Transcript levels of CASP1, NLRP3, and TNF-α were up-regulated in PBMC from all patient groups when compared to healthy subjects. Our results suggest a possible causal link between oxidative stress, high levels of NT and ADMA, and inflammasome activation, which may be involved in the endothelial inflammatory dysfunction leading to the pathogenesis and progression of CHD in PT patients. |
format | Online Article Text |
id | pubmed-8929118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89291182022-06-04 Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients Ferlazzo, Nadia Currò, Monica Isola, Gaetano Maggio, Silvia Bertuccio, Maria Paola Trovato-Salinaro, Angela Matarese, Giovanni Alibrandi, Angela Caccamo, Daniela Ientile, Riccardo Curr Issues Mol Biol Article Patients with cardiovascular disease (CVD) and periodontitis (PT) show shared risk factors as result of the altered molecular mechanisms associated with pathological conditions. The aim of our study was to evaluate if the plasma biomarkers associated with endothelial dysfunction may also be related to alterations in the inflammatory status in peripheral blood mononuclear cells (PBMC). Patients with PT, coronary heart disease (CHD), or both diseases as well as controls were enrolled. Plasma levels of coenzyme Q10 (CoQ10), 3-nitrotyrosine (NT), and asymmetric dimethylarginine (ADMA) were assessed using HPLC. mRNA levels of caspase-1 (CASP1), NLR family pyrin domain containing 3 (NLRP3), and tumor necrosis factor-α (TNF-α) in PBMC from the recruited subjects were quantified using real-time PCR. Patients with PT + CHD showed lower CoQ10 plasma levels and increased concentrations of NT in comparison to healthy subjects. ADMA levels were higher in CHD and PT + CHD patients compared to controls. Transcript levels of CASP1, NLRP3, and TNF-α were up-regulated in PBMC from all patient groups when compared to healthy subjects. Our results suggest a possible causal link between oxidative stress, high levels of NT and ADMA, and inflammasome activation, which may be involved in the endothelial inflammatory dysfunction leading to the pathogenesis and progression of CHD in PT patients. MDPI 2021-07-15 /pmc/articles/PMC8929118/ /pubmed/34287264 http://dx.doi.org/10.3390/cimb43020051 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ferlazzo, Nadia Currò, Monica Isola, Gaetano Maggio, Silvia Bertuccio, Maria Paola Trovato-Salinaro, Angela Matarese, Giovanni Alibrandi, Angela Caccamo, Daniela Ientile, Riccardo Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients |
title | Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients |
title_full | Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients |
title_fullStr | Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients |
title_full_unstemmed | Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients |
title_short | Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients |
title_sort | changes in the biomarkers of oxidative/nitrosative stress and endothelial dysfunction are associated with cardiovascular risk in periodontitis patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929118/ https://www.ncbi.nlm.nih.gov/pubmed/34287264 http://dx.doi.org/10.3390/cimb43020051 |
work_keys_str_mv | AT ferlazzonadia changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT curromonica changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT isolagaetano changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT maggiosilvia changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT bertucciomariapaola changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT trovatosalinaroangela changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT mataresegiovanni changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT alibrandiangela changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT caccamodaniela changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients AT ientilericcardo changesinthebiomarkersofoxidativenitrosativestressandendothelialdysfunctionareassociatedwithcardiovascularriskinperiodontitispatients |