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Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients

Patients with cardiovascular disease (CVD) and periodontitis (PT) show shared risk factors as result of the altered molecular mechanisms associated with pathological conditions. The aim of our study was to evaluate if the plasma biomarkers associated with endothelial dysfunction may also be related...

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Autores principales: Ferlazzo, Nadia, Currò, Monica, Isola, Gaetano, Maggio, Silvia, Bertuccio, Maria Paola, Trovato-Salinaro, Angela, Matarese, Giovanni, Alibrandi, Angela, Caccamo, Daniela, Ientile, Riccardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929118/
https://www.ncbi.nlm.nih.gov/pubmed/34287264
http://dx.doi.org/10.3390/cimb43020051
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author Ferlazzo, Nadia
Currò, Monica
Isola, Gaetano
Maggio, Silvia
Bertuccio, Maria Paola
Trovato-Salinaro, Angela
Matarese, Giovanni
Alibrandi, Angela
Caccamo, Daniela
Ientile, Riccardo
author_facet Ferlazzo, Nadia
Currò, Monica
Isola, Gaetano
Maggio, Silvia
Bertuccio, Maria Paola
Trovato-Salinaro, Angela
Matarese, Giovanni
Alibrandi, Angela
Caccamo, Daniela
Ientile, Riccardo
author_sort Ferlazzo, Nadia
collection PubMed
description Patients with cardiovascular disease (CVD) and periodontitis (PT) show shared risk factors as result of the altered molecular mechanisms associated with pathological conditions. The aim of our study was to evaluate if the plasma biomarkers associated with endothelial dysfunction may also be related to alterations in the inflammatory status in peripheral blood mononuclear cells (PBMC). Patients with PT, coronary heart disease (CHD), or both diseases as well as controls were enrolled. Plasma levels of coenzyme Q10 (CoQ10), 3-nitrotyrosine (NT), and asymmetric dimethylarginine (ADMA) were assessed using HPLC. mRNA levels of caspase-1 (CASP1), NLR family pyrin domain containing 3 (NLRP3), and tumor necrosis factor-α (TNF-α) in PBMC from the recruited subjects were quantified using real-time PCR. Patients with PT + CHD showed lower CoQ10 plasma levels and increased concentrations of NT in comparison to healthy subjects. ADMA levels were higher in CHD and PT + CHD patients compared to controls. Transcript levels of CASP1, NLRP3, and TNF-α were up-regulated in PBMC from all patient groups when compared to healthy subjects. Our results suggest a possible causal link between oxidative stress, high levels of NT and ADMA, and inflammasome activation, which may be involved in the endothelial inflammatory dysfunction leading to the pathogenesis and progression of CHD in PT patients.
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spelling pubmed-89291182022-06-04 Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients Ferlazzo, Nadia Currò, Monica Isola, Gaetano Maggio, Silvia Bertuccio, Maria Paola Trovato-Salinaro, Angela Matarese, Giovanni Alibrandi, Angela Caccamo, Daniela Ientile, Riccardo Curr Issues Mol Biol Article Patients with cardiovascular disease (CVD) and periodontitis (PT) show shared risk factors as result of the altered molecular mechanisms associated with pathological conditions. The aim of our study was to evaluate if the plasma biomarkers associated with endothelial dysfunction may also be related to alterations in the inflammatory status in peripheral blood mononuclear cells (PBMC). Patients with PT, coronary heart disease (CHD), or both diseases as well as controls were enrolled. Plasma levels of coenzyme Q10 (CoQ10), 3-nitrotyrosine (NT), and asymmetric dimethylarginine (ADMA) were assessed using HPLC. mRNA levels of caspase-1 (CASP1), NLR family pyrin domain containing 3 (NLRP3), and tumor necrosis factor-α (TNF-α) in PBMC from the recruited subjects were quantified using real-time PCR. Patients with PT + CHD showed lower CoQ10 plasma levels and increased concentrations of NT in comparison to healthy subjects. ADMA levels were higher in CHD and PT + CHD patients compared to controls. Transcript levels of CASP1, NLRP3, and TNF-α were up-regulated in PBMC from all patient groups when compared to healthy subjects. Our results suggest a possible causal link between oxidative stress, high levels of NT and ADMA, and inflammasome activation, which may be involved in the endothelial inflammatory dysfunction leading to the pathogenesis and progression of CHD in PT patients. MDPI 2021-07-15 /pmc/articles/PMC8929118/ /pubmed/34287264 http://dx.doi.org/10.3390/cimb43020051 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferlazzo, Nadia
Currò, Monica
Isola, Gaetano
Maggio, Silvia
Bertuccio, Maria Paola
Trovato-Salinaro, Angela
Matarese, Giovanni
Alibrandi, Angela
Caccamo, Daniela
Ientile, Riccardo
Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients
title Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients
title_full Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients
title_fullStr Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients
title_full_unstemmed Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients
title_short Changes in the Biomarkers of Oxidative/Nitrosative Stress and Endothelial Dysfunction Are Associated with Cardiovascular Risk in Periodontitis Patients
title_sort changes in the biomarkers of oxidative/nitrosative stress and endothelial dysfunction are associated with cardiovascular risk in periodontitis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929118/
https://www.ncbi.nlm.nih.gov/pubmed/34287264
http://dx.doi.org/10.3390/cimb43020051
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