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Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics
Wastewater stabilization ponds are a natural form of wastewater treatment. Their low operation and maintenance costs have made them popular, especially in developing countries. In these systems, effluents are retained for long periods of time, allowing the microbial communities present in the ponds...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929167/ https://www.ncbi.nlm.nih.gov/pubmed/35310160 http://dx.doi.org/10.7717/peerj.12772 |
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author | Irazoqui, Jose M. Eberhardt, Maria F. Adjad, Maria M. Amadio, Ariel F. |
author_facet | Irazoqui, Jose M. Eberhardt, Maria F. Adjad, Maria M. Amadio, Ariel F. |
author_sort | Irazoqui, Jose M. |
collection | PubMed |
description | Wastewater stabilization ponds are a natural form of wastewater treatment. Their low operation and maintenance costs have made them popular, especially in developing countries. In these systems, effluents are retained for long periods of time, allowing the microbial communities present in the ponds to degrade the organic matter present, using both aerobic and anaerobic processes. Even though these systems are widespread in low income countries, there are no studies about the microorganisms present in them and how they operate. In this study, we analised the microbial communities of two serial full-scale stabilization ponds systems using whole genome shotgun sequencing. First, a taxonomic profiling of the reads was performed, to estimate the microbial diversity. Then, the reads of each system were assembled and binned, allowing the reconstruction of 110 microbial genomes. A functional analysis of the genomes allowed us to find how the main metabolic pathways are carried out, and we propose several organisms that would be key to this kind of environment, since they play an important role in these metabolic pathways. This study represents the first genome-centred approach to understand the metabolic processes in facultative ponds. A better understanding of these microbial communities and how they stabilize the effluents of dairy industries is necessary to improve them and to minimize the environmental impact of dairy industries wastewater. |
format | Online Article Text |
id | pubmed-8929167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89291672022-03-18 Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics Irazoqui, Jose M. Eberhardt, Maria F. Adjad, Maria M. Amadio, Ariel F. PeerJ Bioinformatics Wastewater stabilization ponds are a natural form of wastewater treatment. Their low operation and maintenance costs have made them popular, especially in developing countries. In these systems, effluents are retained for long periods of time, allowing the microbial communities present in the ponds to degrade the organic matter present, using both aerobic and anaerobic processes. Even though these systems are widespread in low income countries, there are no studies about the microorganisms present in them and how they operate. In this study, we analised the microbial communities of two serial full-scale stabilization ponds systems using whole genome shotgun sequencing. First, a taxonomic profiling of the reads was performed, to estimate the microbial diversity. Then, the reads of each system were assembled and binned, allowing the reconstruction of 110 microbial genomes. A functional analysis of the genomes allowed us to find how the main metabolic pathways are carried out, and we propose several organisms that would be key to this kind of environment, since they play an important role in these metabolic pathways. This study represents the first genome-centred approach to understand the metabolic processes in facultative ponds. A better understanding of these microbial communities and how they stabilize the effluents of dairy industries is necessary to improve them and to minimize the environmental impact of dairy industries wastewater. PeerJ Inc. 2022-03-14 /pmc/articles/PMC8929167/ /pubmed/35310160 http://dx.doi.org/10.7717/peerj.12772 Text en © 2022 Irazoqui et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Bioinformatics Irazoqui, Jose M. Eberhardt, Maria F. Adjad, Maria M. Amadio, Ariel F. Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics |
title | Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics |
title_full | Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics |
title_fullStr | Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics |
title_full_unstemmed | Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics |
title_short | Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics |
title_sort | identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics |
topic | Bioinformatics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929167/ https://www.ncbi.nlm.nih.gov/pubmed/35310160 http://dx.doi.org/10.7717/peerj.12772 |
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