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microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing

The growing demands on protein producers and the dwindling available resources have made Hermetia illucens (the black soldier fly, BSF) an economically important species. Insights into the genome of this insect will better allow for robust breeding protocols, and more efficient production to be used...

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Autores principales: DeRaedt, Sarah, Bierman, Anandi, van Heusden, Peter, Richards, Cameron, Christoffels, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929568/
https://www.ncbi.nlm.nih.gov/pubmed/35298540
http://dx.doi.org/10.1371/journal.pone.0265492
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author DeRaedt, Sarah
Bierman, Anandi
van Heusden, Peter
Richards, Cameron
Christoffels, Alan
author_facet DeRaedt, Sarah
Bierman, Anandi
van Heusden, Peter
Richards, Cameron
Christoffels, Alan
author_sort DeRaedt, Sarah
collection PubMed
description The growing demands on protein producers and the dwindling available resources have made Hermetia illucens (the black soldier fly, BSF) an economically important species. Insights into the genome of this insect will better allow for robust breeding protocols, and more efficient production to be used as a replacement of animal feed protein. The use of microRNA as a method to understand how gene regulation allows insect species to adapt to changes in their environment, has been established in multiple species. The baseline and life stage expression levels established in this study, allow for insight into the development and sex-linked microRNA regulation in BSF. To accomplish this, microRNA was extracted and sequenced from 15 different libraries with each life stage in triplicate. Of the total 192 microRNAs found, 168 were orthologous to known arthropod microRNAs and 24 microRNAs were unique to BSF. Twenty-six of the 168 microRNAs conserved across arthropods had a statistically significant (p < 0.05) differential expression between Egg to Larval stages. The development from larva to pupa was characterized by 16 statistically significant differentially expressed microRNA. Seven and 9 microRNA were detected as statistically significant between pupa to adult female and pupa to adult male, respectively. All life stages had a nearly equal split between up and down regulated microRNAs. Ten of the unique 24 miRNA were detected exclusively in one life stage. The egg life stage expressed five microRNA (hil-miR-m, hil-miR-p, hil-miR-r, hil-miR-s, and hil-miR-u) not seen in any other life stages. The female adult and pupa life stages expressed one miRNA each hil-miR-h and hil-miR-ac respectively. Both male and female adult life stages expressed hil-miR-a, hil-miR-b, and hil-miR-y. There were no unique microRNAs found only in the larva stage. Twenty-two microRNAs with 56 experimentally validated target genes in the closely related Drosophila melanogaster were identified. Thus, the microRNA found display the unique evolution of BSF, along with the life stages and potential genes to target for robust mass rearing. Understanding of the microRNA expression in BSF will further their use in the crucial search for alternative and sustainable protein sources.
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spelling pubmed-89295682022-03-18 microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing DeRaedt, Sarah Bierman, Anandi van Heusden, Peter Richards, Cameron Christoffels, Alan PLoS One Research Article The growing demands on protein producers and the dwindling available resources have made Hermetia illucens (the black soldier fly, BSF) an economically important species. Insights into the genome of this insect will better allow for robust breeding protocols, and more efficient production to be used as a replacement of animal feed protein. The use of microRNA as a method to understand how gene regulation allows insect species to adapt to changes in their environment, has been established in multiple species. The baseline and life stage expression levels established in this study, allow for insight into the development and sex-linked microRNA regulation in BSF. To accomplish this, microRNA was extracted and sequenced from 15 different libraries with each life stage in triplicate. Of the total 192 microRNAs found, 168 were orthologous to known arthropod microRNAs and 24 microRNAs were unique to BSF. Twenty-six of the 168 microRNAs conserved across arthropods had a statistically significant (p < 0.05) differential expression between Egg to Larval stages. The development from larva to pupa was characterized by 16 statistically significant differentially expressed microRNA. Seven and 9 microRNA were detected as statistically significant between pupa to adult female and pupa to adult male, respectively. All life stages had a nearly equal split between up and down regulated microRNAs. Ten of the unique 24 miRNA were detected exclusively in one life stage. The egg life stage expressed five microRNA (hil-miR-m, hil-miR-p, hil-miR-r, hil-miR-s, and hil-miR-u) not seen in any other life stages. The female adult and pupa life stages expressed one miRNA each hil-miR-h and hil-miR-ac respectively. Both male and female adult life stages expressed hil-miR-a, hil-miR-b, and hil-miR-y. There were no unique microRNAs found only in the larva stage. Twenty-two microRNAs with 56 experimentally validated target genes in the closely related Drosophila melanogaster were identified. Thus, the microRNA found display the unique evolution of BSF, along with the life stages and potential genes to target for robust mass rearing. Understanding of the microRNA expression in BSF will further their use in the crucial search for alternative and sustainable protein sources. Public Library of Science 2022-03-17 /pmc/articles/PMC8929568/ /pubmed/35298540 http://dx.doi.org/10.1371/journal.pone.0265492 Text en © 2022 DeRaedt et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
DeRaedt, Sarah
Bierman, Anandi
van Heusden, Peter
Richards, Cameron
Christoffels, Alan
microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing
title microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing
title_full microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing
title_fullStr microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing
title_full_unstemmed microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing
title_short microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing
title_sort microrna profile of hermetia illucens (black soldier fly) and its implications on mass rearing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929568/
https://www.ncbi.nlm.nih.gov/pubmed/35298540
http://dx.doi.org/10.1371/journal.pone.0265492
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