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Microfluidics-based strategies for molecular diagnostics of infectious diseases
Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8930194/ https://www.ncbi.nlm.nih.gov/pubmed/35300739 http://dx.doi.org/10.1186/s40779-022-00374-3 |
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author | Wang, Xin Hong, Xian-Zhe Li, Yi-Wei Li, Ying Wang, Jie Chen, Peng Liu, Bi-Feng |
author_facet | Wang, Xin Hong, Xian-Zhe Li, Yi-Wei Li, Ying Wang, Jie Chen, Peng Liu, Bi-Feng |
author_sort | Wang, Xin |
collection | PubMed |
description | Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes, including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis. |
format | Online Article Text |
id | pubmed-8930194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-89301942022-03-18 Microfluidics-based strategies for molecular diagnostics of infectious diseases Wang, Xin Hong, Xian-Zhe Li, Yi-Wei Li, Ying Wang, Jie Chen, Peng Liu, Bi-Feng Mil Med Res Review Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes, including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis. BioMed Central 2022-03-18 /pmc/articles/PMC8930194/ /pubmed/35300739 http://dx.doi.org/10.1186/s40779-022-00374-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Review Wang, Xin Hong, Xian-Zhe Li, Yi-Wei Li, Ying Wang, Jie Chen, Peng Liu, Bi-Feng Microfluidics-based strategies for molecular diagnostics of infectious diseases |
title | Microfluidics-based strategies for molecular diagnostics of infectious diseases |
title_full | Microfluidics-based strategies for molecular diagnostics of infectious diseases |
title_fullStr | Microfluidics-based strategies for molecular diagnostics of infectious diseases |
title_full_unstemmed | Microfluidics-based strategies for molecular diagnostics of infectious diseases |
title_short | Microfluidics-based strategies for molecular diagnostics of infectious diseases |
title_sort | microfluidics-based strategies for molecular diagnostics of infectious diseases |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8930194/ https://www.ncbi.nlm.nih.gov/pubmed/35300739 http://dx.doi.org/10.1186/s40779-022-00374-3 |
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