Origin and genetic analysis of stem rust resistance in wheat line Tr129

Wheat line Tr129 is resistant to stem rust, caused by Puccinia graminis f. sp. tritici (Pgt). The resistance in Tr129 was reportedly derived from Aegilops triuncialis, but the origin and genetics of resistance have not been confirmed. Here, genomic in situ hybridization (GISH) showed that no Ae. tri...

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Autores principales: Sharma, Jyoti Saini, Fetch, Thomas G., Ghazvini, Habibollah, Rouse, Matthew N., Danilova, Tatiana, Friebe, Bernd, Hiebert, Colin W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931155/
https://www.ncbi.nlm.nih.gov/pubmed/35301415
http://dx.doi.org/10.1038/s41598-022-08681-4
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author Sharma, Jyoti Saini
Fetch, Thomas G.
Ghazvini, Habibollah
Rouse, Matthew N.
Danilova, Tatiana
Friebe, Bernd
Hiebert, Colin W.
author_facet Sharma, Jyoti Saini
Fetch, Thomas G.
Ghazvini, Habibollah
Rouse, Matthew N.
Danilova, Tatiana
Friebe, Bernd
Hiebert, Colin W.
author_sort Sharma, Jyoti Saini
collection PubMed
description Wheat line Tr129 is resistant to stem rust, caused by Puccinia graminis f. sp. tritici (Pgt). The resistance in Tr129 was reportedly derived from Aegilops triuncialis, but the origin and genetics of resistance have not been confirmed. Here, genomic in situ hybridization (GISH) showed that no Ae. triuncialis chromatin was present in Tr129. Genetic and phenotypic analysis was conducted on F(2) and DH populations from the cross RL6071/Tr129. Seedlings were tested with six Pgt races and were genotyped using an Illumina iSelect 90 K SNP array and kompetitive allele specific PCR (KASP) markers. Mapping and phenotyping showed that Tr129 carried four stem rust resistance (Sr) genes on chromosome arms 2BL (Sr9b), 4AL (Sr7b), 6AS (Sr8a), and 6DS (SrTr129). SrTr129 co-segregated with markers for SrCad, however Tr129 has a unique haplotype suggesting the resistance could be new. Analysis of a RL6071/Peace population revealed that like SrTr129, SrCad is ineffective against three North American races. This new understanding of SrCad will guide its use in breeding. Tr129 and the DNA markers reported here are useful resources for improving stem rust resistance in cultivars.
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spelling pubmed-89311552022-03-21 Origin and genetic analysis of stem rust resistance in wheat line Tr129 Sharma, Jyoti Saini Fetch, Thomas G. Ghazvini, Habibollah Rouse, Matthew N. Danilova, Tatiana Friebe, Bernd Hiebert, Colin W. Sci Rep Article Wheat line Tr129 is resistant to stem rust, caused by Puccinia graminis f. sp. tritici (Pgt). The resistance in Tr129 was reportedly derived from Aegilops triuncialis, but the origin and genetics of resistance have not been confirmed. Here, genomic in situ hybridization (GISH) showed that no Ae. triuncialis chromatin was present in Tr129. Genetic and phenotypic analysis was conducted on F(2) and DH populations from the cross RL6071/Tr129. Seedlings were tested with six Pgt races and were genotyped using an Illumina iSelect 90 K SNP array and kompetitive allele specific PCR (KASP) markers. Mapping and phenotyping showed that Tr129 carried four stem rust resistance (Sr) genes on chromosome arms 2BL (Sr9b), 4AL (Sr7b), 6AS (Sr8a), and 6DS (SrTr129). SrTr129 co-segregated with markers for SrCad, however Tr129 has a unique haplotype suggesting the resistance could be new. Analysis of a RL6071/Peace population revealed that like SrTr129, SrCad is ineffective against three North American races. This new understanding of SrCad will guide its use in breeding. Tr129 and the DNA markers reported here are useful resources for improving stem rust resistance in cultivars. Nature Publishing Group UK 2022-03-17 /pmc/articles/PMC8931155/ /pubmed/35301415 http://dx.doi.org/10.1038/s41598-022-08681-4 Text en © Crown 2022 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sharma, Jyoti Saini
Fetch, Thomas G.
Ghazvini, Habibollah
Rouse, Matthew N.
Danilova, Tatiana
Friebe, Bernd
Hiebert, Colin W.
Origin and genetic analysis of stem rust resistance in wheat line Tr129
title Origin and genetic analysis of stem rust resistance in wheat line Tr129
title_full Origin and genetic analysis of stem rust resistance in wheat line Tr129
title_fullStr Origin and genetic analysis of stem rust resistance in wheat line Tr129
title_full_unstemmed Origin and genetic analysis of stem rust resistance in wheat line Tr129
title_short Origin and genetic analysis of stem rust resistance in wheat line Tr129
title_sort origin and genetic analysis of stem rust resistance in wheat line tr129
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931155/
https://www.ncbi.nlm.nih.gov/pubmed/35301415
http://dx.doi.org/10.1038/s41598-022-08681-4
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