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Development of Novel Dengue NS1 Multiplex Lateral Flow Immunoassay to Differentiate Serotypes in Serum of Acute Phase Patients and Infected Mosquitoes

Dengue is among the most rapidly spreading arboviral disease in the world. A low-cost, easy to use point-of-care diagnostic tool for the detection and differentiation of dengue virus serotypes could improve clinical management, disease prevention, epidemiological surveillance, and outbreak monitorin...

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Autores principales: Lai, Szu-Chia, Huang, Yu-Yine, Wey, Jiunn-Jye, Tsai, Meng-Hung, Chen, Yi-Ling, Shu, Pei-Yun, Chang, Shu-Fen, Hung, Yi-Jen, Hou, Jiu-Nan, Lin, Chang-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931297/
https://www.ncbi.nlm.nih.gov/pubmed/35309328
http://dx.doi.org/10.3389/fimmu.2022.852452
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author Lai, Szu-Chia
Huang, Yu-Yine
Wey, Jiunn-Jye
Tsai, Meng-Hung
Chen, Yi-Ling
Shu, Pei-Yun
Chang, Shu-Fen
Hung, Yi-Jen
Hou, Jiu-Nan
Lin, Chang-Chi
author_facet Lai, Szu-Chia
Huang, Yu-Yine
Wey, Jiunn-Jye
Tsai, Meng-Hung
Chen, Yi-Ling
Shu, Pei-Yun
Chang, Shu-Fen
Hung, Yi-Jen
Hou, Jiu-Nan
Lin, Chang-Chi
author_sort Lai, Szu-Chia
collection PubMed
description Dengue is among the most rapidly spreading arboviral disease in the world. A low-cost, easy to use point-of-care diagnostic tool for the detection and differentiation of dengue virus serotypes could improve clinical management, disease prevention, epidemiological surveillance, and outbreak monitoring, particularly in regions where multiple serotypes co-circulate. Despite widespread deployment, no commercial dengue antigen diagnostic test has proven effective in differentiating among dengue virus serotypes. In the current study, we first established mAb pairs and developed a multiplex lateral flow immunoassay for the simultaneous detection of the dengue viral NS1 antigen and identification of serotype. The proposed system, called Dengue serotype NS1 Multiplex LFIA, provides high sensitivity and specificity. In testing for JEV, ZIKV, YFV, WNV, and CHIKV, the multiplex LFIA gave no indication of cross- reactivity with cell culture supernatants of other flaviviruses or chikungunya virus. In analyzing 187 samples from patients suspected of dengue infection, the detection sensitivity for serotype D1 to D4 was 90.0%, 88.24%, 82.61%, and 83.33% and serotype specificity was 98.74%, 96.13%, 99.39%, and 97.04%, respectively. Our multiplex LFIA can also identify mono- and co-infection of different serotype of dengue viruses in mosquitoes. The proposed Multiplex LFIA provides a simple tool for the rapid detection of dengue serotypes and in the differential diagnosis of fever patients in regions where medical resources are limited and/or multiple DENVs co-circulate.
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spelling pubmed-89312972022-03-19 Development of Novel Dengue NS1 Multiplex Lateral Flow Immunoassay to Differentiate Serotypes in Serum of Acute Phase Patients and Infected Mosquitoes Lai, Szu-Chia Huang, Yu-Yine Wey, Jiunn-Jye Tsai, Meng-Hung Chen, Yi-Ling Shu, Pei-Yun Chang, Shu-Fen Hung, Yi-Jen Hou, Jiu-Nan Lin, Chang-Chi Front Immunol Immunology Dengue is among the most rapidly spreading arboviral disease in the world. A low-cost, easy to use point-of-care diagnostic tool for the detection and differentiation of dengue virus serotypes could improve clinical management, disease prevention, epidemiological surveillance, and outbreak monitoring, particularly in regions where multiple serotypes co-circulate. Despite widespread deployment, no commercial dengue antigen diagnostic test has proven effective in differentiating among dengue virus serotypes. In the current study, we first established mAb pairs and developed a multiplex lateral flow immunoassay for the simultaneous detection of the dengue viral NS1 antigen and identification of serotype. The proposed system, called Dengue serotype NS1 Multiplex LFIA, provides high sensitivity and specificity. In testing for JEV, ZIKV, YFV, WNV, and CHIKV, the multiplex LFIA gave no indication of cross- reactivity with cell culture supernatants of other flaviviruses or chikungunya virus. In analyzing 187 samples from patients suspected of dengue infection, the detection sensitivity for serotype D1 to D4 was 90.0%, 88.24%, 82.61%, and 83.33% and serotype specificity was 98.74%, 96.13%, 99.39%, and 97.04%, respectively. Our multiplex LFIA can also identify mono- and co-infection of different serotype of dengue viruses in mosquitoes. The proposed Multiplex LFIA provides a simple tool for the rapid detection of dengue serotypes and in the differential diagnosis of fever patients in regions where medical resources are limited and/or multiple DENVs co-circulate. Frontiers Media S.A. 2022-03-04 /pmc/articles/PMC8931297/ /pubmed/35309328 http://dx.doi.org/10.3389/fimmu.2022.852452 Text en Copyright © 2022 Lai, Huang, Wey, Tsai, Chen, Shu, Chang, Hung, Hou and Lin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Lai, Szu-Chia
Huang, Yu-Yine
Wey, Jiunn-Jye
Tsai, Meng-Hung
Chen, Yi-Ling
Shu, Pei-Yun
Chang, Shu-Fen
Hung, Yi-Jen
Hou, Jiu-Nan
Lin, Chang-Chi
Development of Novel Dengue NS1 Multiplex Lateral Flow Immunoassay to Differentiate Serotypes in Serum of Acute Phase Patients and Infected Mosquitoes
title Development of Novel Dengue NS1 Multiplex Lateral Flow Immunoassay to Differentiate Serotypes in Serum of Acute Phase Patients and Infected Mosquitoes
title_full Development of Novel Dengue NS1 Multiplex Lateral Flow Immunoassay to Differentiate Serotypes in Serum of Acute Phase Patients and Infected Mosquitoes
title_fullStr Development of Novel Dengue NS1 Multiplex Lateral Flow Immunoassay to Differentiate Serotypes in Serum of Acute Phase Patients and Infected Mosquitoes
title_full_unstemmed Development of Novel Dengue NS1 Multiplex Lateral Flow Immunoassay to Differentiate Serotypes in Serum of Acute Phase Patients and Infected Mosquitoes
title_short Development of Novel Dengue NS1 Multiplex Lateral Flow Immunoassay to Differentiate Serotypes in Serum of Acute Phase Patients and Infected Mosquitoes
title_sort development of novel dengue ns1 multiplex lateral flow immunoassay to differentiate serotypes in serum of acute phase patients and infected mosquitoes
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931297/
https://www.ncbi.nlm.nih.gov/pubmed/35309328
http://dx.doi.org/10.3389/fimmu.2022.852452
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