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FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis

Meiotic prophase I is a prolonged G2 phase that ensures the completion of numerous meiosis-specific chromosome events. During meiotic prophase I, homologous chromosomes undergo synapsis to facilitate meiotic recombination yielding crossovers. It remains largely elusive how homolog synapsis is tempor...

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Autores principales: Tanno, Nobuhiro, Takemoto, Kazumasa, Takada-Horisawa, Yuki, Shimada, Ryuki, Fujimura, Sayoko, Tani, Naoki, Takeda, Naoki, Araki, Kimi, Ishiguro, Kei-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931362/
https://www.ncbi.nlm.nih.gov/pubmed/35310947
http://dx.doi.org/10.1016/j.isci.2022.104008
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author Tanno, Nobuhiro
Takemoto, Kazumasa
Takada-Horisawa, Yuki
Shimada, Ryuki
Fujimura, Sayoko
Tani, Naoki
Takeda, Naoki
Araki, Kimi
Ishiguro, Kei-ichiro
author_facet Tanno, Nobuhiro
Takemoto, Kazumasa
Takada-Horisawa, Yuki
Shimada, Ryuki
Fujimura, Sayoko
Tani, Naoki
Takeda, Naoki
Araki, Kimi
Ishiguro, Kei-ichiro
author_sort Tanno, Nobuhiro
collection PubMed
description Meiotic prophase I is a prolonged G2 phase that ensures the completion of numerous meiosis-specific chromosome events. During meiotic prophase I, homologous chromosomes undergo synapsis to facilitate meiotic recombination yielding crossovers. It remains largely elusive how homolog synapsis is temporally maintained and destabilized during meiotic prophase I. Here we show that FBXO47 is the stabilizer of the synaptonemal complex during male meiotic prophase I. Disruption of FBXO47 shows severe impact on homologous chromosome synapsis, meiotic recombination, and XY body formation, leading to male infertility. Notably, in the absence of FBXO47, although once homologous chromosomes are synapsed, the synaptonemal complex is precociously disassembled before progressing beyond pachytene. Remarkably, Fbxo47 KO spermatocytes remain in an earlier stage of meiotic prophase I and lack crossovers, despite apparently exhibiting diplotene-like chromosome morphology. We propose that FBXO47 plays a crucial role in preventing the synaptonemal complex from premature disassembly during cell cycle progression of meiotic prophase I.
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spelling pubmed-89313622022-03-19 FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis Tanno, Nobuhiro Takemoto, Kazumasa Takada-Horisawa, Yuki Shimada, Ryuki Fujimura, Sayoko Tani, Naoki Takeda, Naoki Araki, Kimi Ishiguro, Kei-ichiro iScience Article Meiotic prophase I is a prolonged G2 phase that ensures the completion of numerous meiosis-specific chromosome events. During meiotic prophase I, homologous chromosomes undergo synapsis to facilitate meiotic recombination yielding crossovers. It remains largely elusive how homolog synapsis is temporally maintained and destabilized during meiotic prophase I. Here we show that FBXO47 is the stabilizer of the synaptonemal complex during male meiotic prophase I. Disruption of FBXO47 shows severe impact on homologous chromosome synapsis, meiotic recombination, and XY body formation, leading to male infertility. Notably, in the absence of FBXO47, although once homologous chromosomes are synapsed, the synaptonemal complex is precociously disassembled before progressing beyond pachytene. Remarkably, Fbxo47 KO spermatocytes remain in an earlier stage of meiotic prophase I and lack crossovers, despite apparently exhibiting diplotene-like chromosome morphology. We propose that FBXO47 plays a crucial role in preventing the synaptonemal complex from premature disassembly during cell cycle progression of meiotic prophase I. Elsevier 2022-03-01 /pmc/articles/PMC8931362/ /pubmed/35310947 http://dx.doi.org/10.1016/j.isci.2022.104008 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Tanno, Nobuhiro
Takemoto, Kazumasa
Takada-Horisawa, Yuki
Shimada, Ryuki
Fujimura, Sayoko
Tani, Naoki
Takeda, Naoki
Araki, Kimi
Ishiguro, Kei-ichiro
FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
title FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
title_full FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
title_fullStr FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
title_full_unstemmed FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
title_short FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
title_sort fbxo47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931362/
https://www.ncbi.nlm.nih.gov/pubmed/35310947
http://dx.doi.org/10.1016/j.isci.2022.104008
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