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Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020
Human rhinoviruses (HRVs) cause acute upper and lower respiratory tract infections and aggravation of asthma and chronic obstructive pulmonary disease. The 5’ untranslated region (5' UTR) and the VP4/VP2 region are widely used for genotyping of HRVs. Members of the species Rhinovirus A and Rhin...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Vienna
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931777/ https://www.ncbi.nlm.nih.gov/pubmed/35303167 http://dx.doi.org/10.1007/s00705-022-05405-x |
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author | Jiang, Haixia Yang, Tianyun Yang, Chunyi Lu, Yanping Yi, Zhigang Zhang, Qin Wang, Wei |
author_facet | Jiang, Haixia Yang, Tianyun Yang, Chunyi Lu, Yanping Yi, Zhigang Zhang, Qin Wang, Wei |
author_sort | Jiang, Haixia |
collection | PubMed |
description | Human rhinoviruses (HRVs) cause acute upper and lower respiratory tract infections and aggravation of asthma and chronic obstructive pulmonary disease. The 5’ untranslated region (5' UTR) and the VP4/VP2 region are widely used for genotyping of HRVs. Members of the species Rhinovirus A and Rhinovirus C have been reported to be more frequently associated with severe disease than members of the species Rhinovirus B. We report the clinical and molecular epidemiological characteristics of HRVs circulating from 2012 to 2020 in Shanghai. A total of 5832 nasopharyngeal swabs from patients with acute respiratory infections were collected. A real-time reverse transcription polymerase chain reaction assay was used for virus detection. The 5' untranslated region and VP4/VP2 region were amplified and sequenced for genotyping and phylogenetic analysis. The overall rate of rhinovirus detection was 2.74% (160/5832), with members of species A, B, and C accounting for 68.13% (109/160), 20.00% (32/160), and 11.88% (19/160) of the total, respectively. A peak of HRV infection was observed in autumn (5.34%, 58/1087). Patients in the 3- to 14-year-old age group were the most susceptible to HRV infection (χ(2) = 23.88, P = 0.017). Influenza virus and Streptococcus pneumoniae were detected more frequently than other pathogens in cases of coinfection. Recombination events were identified in 10 strains, which were successfully genotyped by phylogenetic analysis based on the 5’ UTR-VP4/VP2 region but not the 5’ UTR region alone. We observed a high degree of variability in the relative distribution of HRV genotypes and the prevalence of HRV infection in Shanghai and found evidence of recombination events in the portion of the genome containing the 5’ UTR and the VP4/VP2 region between HRV-C strains and HRV-A-like strains. This study is important for surveillance of the spread of HRVs and the emergence of new variants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-022-05405-x. |
format | Online Article Text |
id | pubmed-8931777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-89317772022-03-18 Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020 Jiang, Haixia Yang, Tianyun Yang, Chunyi Lu, Yanping Yi, Zhigang Zhang, Qin Wang, Wei Arch Virol Original Article Human rhinoviruses (HRVs) cause acute upper and lower respiratory tract infections and aggravation of asthma and chronic obstructive pulmonary disease. The 5’ untranslated region (5' UTR) and the VP4/VP2 region are widely used for genotyping of HRVs. Members of the species Rhinovirus A and Rhinovirus C have been reported to be more frequently associated with severe disease than members of the species Rhinovirus B. We report the clinical and molecular epidemiological characteristics of HRVs circulating from 2012 to 2020 in Shanghai. A total of 5832 nasopharyngeal swabs from patients with acute respiratory infections were collected. A real-time reverse transcription polymerase chain reaction assay was used for virus detection. The 5' untranslated region and VP4/VP2 region were amplified and sequenced for genotyping and phylogenetic analysis. The overall rate of rhinovirus detection was 2.74% (160/5832), with members of species A, B, and C accounting for 68.13% (109/160), 20.00% (32/160), and 11.88% (19/160) of the total, respectively. A peak of HRV infection was observed in autumn (5.34%, 58/1087). Patients in the 3- to 14-year-old age group were the most susceptible to HRV infection (χ(2) = 23.88, P = 0.017). Influenza virus and Streptococcus pneumoniae were detected more frequently than other pathogens in cases of coinfection. Recombination events were identified in 10 strains, which were successfully genotyped by phylogenetic analysis based on the 5’ UTR-VP4/VP2 region but not the 5’ UTR region alone. We observed a high degree of variability in the relative distribution of HRV genotypes and the prevalence of HRV infection in Shanghai and found evidence of recombination events in the portion of the genome containing the 5’ UTR and the VP4/VP2 region between HRV-C strains and HRV-A-like strains. This study is important for surveillance of the spread of HRVs and the emergence of new variants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-022-05405-x. Springer Vienna 2022-03-18 2022 /pmc/articles/PMC8931777/ /pubmed/35303167 http://dx.doi.org/10.1007/s00705-022-05405-x Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Jiang, Haixia Yang, Tianyun Yang, Chunyi Lu, Yanping Yi, Zhigang Zhang, Qin Wang, Wei Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020 |
title | Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020 |
title_full | Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020 |
title_fullStr | Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020 |
title_full_unstemmed | Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020 |
title_short | Molecular epidemiology and clinical characterization of human rhinoviruses circulating in Shanghai, 2012-2020 |
title_sort | molecular epidemiology and clinical characterization of human rhinoviruses circulating in shanghai, 2012-2020 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931777/ https://www.ncbi.nlm.nih.gov/pubmed/35303167 http://dx.doi.org/10.1007/s00705-022-05405-x |
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