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Skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults

BACKGROUND: Myostatin (MSTN) is a key negative regulator of muscle mass in humans and animals, having direct and indirect influences on molecular regulators of atrophy and hypertrophy, thus potentially impacting fitness and physical function. We have shown that myostatin is elevated in conditions of...

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Autores principales: Ryan, Alice S., Li, Guoyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932637/
https://www.ncbi.nlm.nih.gov/pubmed/35311023
http://dx.doi.org/10.1002/crt2.43
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author Ryan, Alice S.
Li, Guoyan
author_facet Ryan, Alice S.
Li, Guoyan
author_sort Ryan, Alice S.
collection PubMed
description BACKGROUND: Myostatin (MSTN) is a key negative regulator of muscle mass in humans and animals, having direct and indirect influences on molecular regulators of atrophy and hypertrophy, thus potentially impacting fitness and physical function. We have shown that myostatin is elevated in conditions of chronic disability (e.g. paretic limb of stroke). Our hypothesis is that myostatin would be elevated in older adults with sarcopenia. The purpose of this study was to examine the role of skeletal muscle myostatin in sarcopenia. METHODS: Sixty-four overweight to obese aged 45–81 years underwent a maximal aerobic capacity (VO(2)max) test, dual-energy X-ray absorptiometry (DXA) scan to determine appendicular lean tissue (ALM), and vastus lateralis muscle biopsy to determine myostatin mRNA expression by quantitative real time PCR (Q-RT-PCR). Rates of sarcopenia were determined using (ALM/BMI), and sarcopenia was defined as <0.789 in men and <0.512 in women. Subjects had low fitness (VO(2)max: 22.7 ± 0.7 mL/kg/min) and on average 40.9 ± 1% body fat. RESULTS: The prevalence of sarcopenia in this cohort was 16%. BMI, % body fat, and fat mass were higher in adults with sarcopenia than those without sarcopenia (all P < 0.001). Myostatin mRNA expression was lower in those without sarcopenia than those with sarcopenia (P < 0.05) and higher in men than women (P < 0.001). Myostatin expression was associated with BMI (r = 0.36, P < 0.01) and mid-thigh intramuscular fat (r = 0.29, P < 0.05). CONCLUSION: Given that myostatin is important in muscle atrophy, fat accumulation, and sarcopenia, further work could address its implication in other aging cohorts of disability and chronic disease.
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spelling pubmed-89326372022-10-01 Skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults Ryan, Alice S. Li, Guoyan JCSM Clin Rep Article BACKGROUND: Myostatin (MSTN) is a key negative regulator of muscle mass in humans and animals, having direct and indirect influences on molecular regulators of atrophy and hypertrophy, thus potentially impacting fitness and physical function. We have shown that myostatin is elevated in conditions of chronic disability (e.g. paretic limb of stroke). Our hypothesis is that myostatin would be elevated in older adults with sarcopenia. The purpose of this study was to examine the role of skeletal muscle myostatin in sarcopenia. METHODS: Sixty-four overweight to obese aged 45–81 years underwent a maximal aerobic capacity (VO(2)max) test, dual-energy X-ray absorptiometry (DXA) scan to determine appendicular lean tissue (ALM), and vastus lateralis muscle biopsy to determine myostatin mRNA expression by quantitative real time PCR (Q-RT-PCR). Rates of sarcopenia were determined using (ALM/BMI), and sarcopenia was defined as <0.789 in men and <0.512 in women. Subjects had low fitness (VO(2)max: 22.7 ± 0.7 mL/kg/min) and on average 40.9 ± 1% body fat. RESULTS: The prevalence of sarcopenia in this cohort was 16%. BMI, % body fat, and fat mass were higher in adults with sarcopenia than those without sarcopenia (all P < 0.001). Myostatin mRNA expression was lower in those without sarcopenia than those with sarcopenia (P < 0.05) and higher in men than women (P < 0.001). Myostatin expression was associated with BMI (r = 0.36, P < 0.01) and mid-thigh intramuscular fat (r = 0.29, P < 0.05). CONCLUSION: Given that myostatin is important in muscle atrophy, fat accumulation, and sarcopenia, further work could address its implication in other aging cohorts of disability and chronic disease. 2021-10 2021-09-23 /pmc/articles/PMC8932637/ /pubmed/35311023 http://dx.doi.org/10.1002/crt2.43 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Article
Ryan, Alice S.
Li, Guoyan
Skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults
title Skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults
title_full Skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults
title_fullStr Skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults
title_full_unstemmed Skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults
title_short Skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults
title_sort skeletal muscle myostatin gene expression and sarcopenia in overweight and obese middle-aged and older adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932637/
https://www.ncbi.nlm.nih.gov/pubmed/35311023
http://dx.doi.org/10.1002/crt2.43
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