PDMS hydrogel-coated tissue culture plates for studying the impact of substrate stiffness on dendritic cell function

The mechanical properties of polydimethylsiloxane hydrogels can be tuned to mimic physiological tensions, an underappreciated environmental parameter in immunology studies. We describe a workflow to prepare PDMS-coated tissue culture plates with biologically relevant substrate stiffness, and the use...

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Detalles Bibliográficos
Autores principales: Lee, Megan, Chu, Kevin, Chakraborty, Mainak, Kotoulas, Nicholas, Akbari, Masoud, Goh, Cynthia, Clemente-Casares, Xavier, Winer, Daniel A., Shrestha, Annie, Tsai, Sue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8933837/
https://www.ncbi.nlm.nih.gov/pubmed/35313712
http://dx.doi.org/10.1016/j.xpro.2022.101233

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