Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells

Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-re...

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Detalles Bibliográficos
Autores principales: Santos, Renata, Mei, Arianna, Marchetto, Maria C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8933838/
https://www.ncbi.nlm.nih.gov/pubmed/35313707
http://dx.doi.org/10.1016/j.xpro.2022.101261
Descripción
Sumario:Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored. For complete details on the use and execution of this protocol, please refer to Santos et al. (2017) and Vadodaria et al. (2021).

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