Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells

Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-re...

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Detalles Bibliográficos
Autores principales: Santos, Renata, Mei, Arianna, Marchetto, Maria C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8933838/
https://www.ncbi.nlm.nih.gov/pubmed/35313707
http://dx.doi.org/10.1016/j.xpro.2022.101261
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author Santos, Renata
Mei, Arianna
Marchetto, Maria C.
author_facet Santos, Renata
Mei, Arianna
Marchetto, Maria C.
author_sort Santos, Renata
collection PubMed
description Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored. For complete details on the use and execution of this protocol, please refer to Santos et al. (2017) and Vadodaria et al. (2021).
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spelling pubmed-89338382022-03-20 Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells Santos, Renata Mei, Arianna Marchetto, Maria C. STAR Protoc Protocol Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored. For complete details on the use and execution of this protocol, please refer to Santos et al. (2017) and Vadodaria et al. (2021). Elsevier 2022-03-17 /pmc/articles/PMC8933838/ /pubmed/35313707 http://dx.doi.org/10.1016/j.xpro.2022.101261 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Santos, Renata
Mei, Arianna
Marchetto, Maria C.
Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
title Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
title_full Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
title_fullStr Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
title_full_unstemmed Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
title_short Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
title_sort generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8933838/
https://www.ncbi.nlm.nih.gov/pubmed/35313707
http://dx.doi.org/10.1016/j.xpro.2022.101261
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