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Growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by AMPK-PGC1α-PPAR signaling pathway

BACKGROUND: Adipose tissue is an important endocrine and energy-storage organ in organisms, and it plays a crucial role in the energy-metabolism balance. Previous studies have found that sex-linked dwarf (SLD) chickens generally have excessively high abdominal fat deposition during the growing perio...

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Autores principales: Yang, Minmin, Hu, Bowen, Sun, Donglei, Zhao, Changbin, Wei, Haohui, Li, Dajian, Liao, Zhiying, Zhao, Yongxia, Liang, Jinping, Shi, Meiqing, Luo, Qingbin, Nie, Qinghua, Zhang, Xiquan, Zhang, Dexiang, Li, Hongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8933938/
https://www.ncbi.nlm.nih.gov/pubmed/35305578
http://dx.doi.org/10.1186/s12864-021-08268-9
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author Yang, Minmin
Hu, Bowen
Sun, Donglei
Zhao, Changbin
Wei, Haohui
Li, Dajian
Liao, Zhiying
Zhao, Yongxia
Liang, Jinping
Shi, Meiqing
Luo, Qingbin
Nie, Qinghua
Zhang, Xiquan
Zhang, Dexiang
Li, Hongmei
author_facet Yang, Minmin
Hu, Bowen
Sun, Donglei
Zhao, Changbin
Wei, Haohui
Li, Dajian
Liao, Zhiying
Zhao, Yongxia
Liang, Jinping
Shi, Meiqing
Luo, Qingbin
Nie, Qinghua
Zhang, Xiquan
Zhang, Dexiang
Li, Hongmei
author_sort Yang, Minmin
collection PubMed
description BACKGROUND: Adipose tissue is an important endocrine and energy-storage organ in organisms, and it plays a crucial role in the energy-metabolism balance. Previous studies have found that sex-linked dwarf (SLD) chickens generally have excessively high abdominal fat deposition during the growing period, which increases feeding costs. However, the underlying mechanism of this fat deposition during the growth of SLD chickens remains unknown. RESULTS: The Oil Red O staining showed that the lipid-droplet area of SLD chickens was larger than that of normal chickens in E15 and 14d. Consistently, TG content in the livers of SLD chickens was higher than that of normal chickens in E15 and 14d. Further, lower ΔΨm and lower ATP levels and higher MDA levels were observed in SLD chickens than normal chickens in both E15 and 14d. We also found that overexpression of GHR reduced the expression of genes related to lipid metabolism (AMPK, PGC1α, PPARγ, FAS, C/EBP) and oxidative phosphorylation (CYTB, CYTC, COX1, ATP), as well as reducing ΔΨm and ATP levels and increasing MDA levels. In addition, overexpression of GHR inhibited fat deposition in CPPAs, as measured by Oil Red O staining. On the contrary, knockdown of GHR had the opposite effects in vitro. CONCLUSIONS: In summary, we demonstrate that GHR promotes mitochondrial function and inhibits lipid peroxidation as well as fat deposition in vivo and in vitro. Therefore, GHR is essential for maintaining the stability of lipid metabolism and regulating mitochondrial function in chicken. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08268-9.
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spelling pubmed-89339382022-03-23 Growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by AMPK-PGC1α-PPAR signaling pathway Yang, Minmin Hu, Bowen Sun, Donglei Zhao, Changbin Wei, Haohui Li, Dajian Liao, Zhiying Zhao, Yongxia Liang, Jinping Shi, Meiqing Luo, Qingbin Nie, Qinghua Zhang, Xiquan Zhang, Dexiang Li, Hongmei BMC Genomics Research Article BACKGROUND: Adipose tissue is an important endocrine and energy-storage organ in organisms, and it plays a crucial role in the energy-metabolism balance. Previous studies have found that sex-linked dwarf (SLD) chickens generally have excessively high abdominal fat deposition during the growing period, which increases feeding costs. However, the underlying mechanism of this fat deposition during the growth of SLD chickens remains unknown. RESULTS: The Oil Red O staining showed that the lipid-droplet area of SLD chickens was larger than that of normal chickens in E15 and 14d. Consistently, TG content in the livers of SLD chickens was higher than that of normal chickens in E15 and 14d. Further, lower ΔΨm and lower ATP levels and higher MDA levels were observed in SLD chickens than normal chickens in both E15 and 14d. We also found that overexpression of GHR reduced the expression of genes related to lipid metabolism (AMPK, PGC1α, PPARγ, FAS, C/EBP) and oxidative phosphorylation (CYTB, CYTC, COX1, ATP), as well as reducing ΔΨm and ATP levels and increasing MDA levels. In addition, overexpression of GHR inhibited fat deposition in CPPAs, as measured by Oil Red O staining. On the contrary, knockdown of GHR had the opposite effects in vitro. CONCLUSIONS: In summary, we demonstrate that GHR promotes mitochondrial function and inhibits lipid peroxidation as well as fat deposition in vivo and in vitro. Therefore, GHR is essential for maintaining the stability of lipid metabolism and regulating mitochondrial function in chicken. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08268-9. BioMed Central 2022-03-19 /pmc/articles/PMC8933938/ /pubmed/35305578 http://dx.doi.org/10.1186/s12864-021-08268-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Yang, Minmin
Hu, Bowen
Sun, Donglei
Zhao, Changbin
Wei, Haohui
Li, Dajian
Liao, Zhiying
Zhao, Yongxia
Liang, Jinping
Shi, Meiqing
Luo, Qingbin
Nie, Qinghua
Zhang, Xiquan
Zhang, Dexiang
Li, Hongmei
Growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by AMPK-PGC1α-PPAR signaling pathway
title Growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by AMPK-PGC1α-PPAR signaling pathway
title_full Growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by AMPK-PGC1α-PPAR signaling pathway
title_fullStr Growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by AMPK-PGC1α-PPAR signaling pathway
title_full_unstemmed Growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by AMPK-PGC1α-PPAR signaling pathway
title_short Growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by AMPK-PGC1α-PPAR signaling pathway
title_sort growth hormone receptor gene influences mitochondrial function and chicken lipid metabolism by ampk-pgc1α-ppar signaling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8933938/
https://www.ncbi.nlm.nih.gov/pubmed/35305578
http://dx.doi.org/10.1186/s12864-021-08268-9
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