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Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae
With the increase of human activities, cadmium (Cd) pollution has become a global environmental problem affecting biological metabolism in ecosystem. Cd has a very long half-life in humans and is excreted slowly in organs, which poses a serious threat to human health. In order to better understand t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934349/ https://www.ncbi.nlm.nih.gov/pubmed/35307728 http://dx.doi.org/10.1038/s41598-022-08758-0 |
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author | Yang, Pingping Yang, Xingran Sun, Liran Han, Xiaobing Xu, Lu Gu, Wei Zhang, Min |
author_facet | Yang, Pingping Yang, Xingran Sun, Liran Han, Xiaobing Xu, Lu Gu, Wei Zhang, Min |
author_sort | Yang, Pingping |
collection | PubMed |
description | With the increase of human activities, cadmium (Cd) pollution has become a global environmental problem affecting biological metabolism in ecosystem. Cd has a very long half-life in humans and is excreted slowly in organs, which poses a serious threat to human health. In order to better understand the toxicity effects of cadmium, third instar larvae of Drosophila melanogaster (Canton-S strain) were exposed to different concentrations (1.125 mg/kg, 2.25 mg/kg, 4.5 mg/kg, and 9 mg/kg) of cadmium. Trypan blue staining showed that intestinal cell damage of Drosophila larvae increased and the comet assay indicated significantly more DNA damage in larvae exposed to high Cd concentrations. The nitroblue tetrazolium (NBT) experiments proved that content of reactive oxygen species (ROS) increased, which indicated Cd exposure could induce oxidative stress. In addition, the expression of mitochondrial adenine nucleotide transferase coding gene (sesB and Ant2) and apoptosis related genes (Debcl, hid, rpr, p53, Sce and Diap1) changed, which may lead to increased apoptosis. These findings confirmed the toxicity effects on oxidative stress and cell apoptosis in Drosophila larvae after early cadmium exposure, providing insights into understanding the effects of heavy metal stress in animal development. |
format | Online Article Text |
id | pubmed-8934349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89343492022-03-28 Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae Yang, Pingping Yang, Xingran Sun, Liran Han, Xiaobing Xu, Lu Gu, Wei Zhang, Min Sci Rep Article With the increase of human activities, cadmium (Cd) pollution has become a global environmental problem affecting biological metabolism in ecosystem. Cd has a very long half-life in humans and is excreted slowly in organs, which poses a serious threat to human health. In order to better understand the toxicity effects of cadmium, third instar larvae of Drosophila melanogaster (Canton-S strain) were exposed to different concentrations (1.125 mg/kg, 2.25 mg/kg, 4.5 mg/kg, and 9 mg/kg) of cadmium. Trypan blue staining showed that intestinal cell damage of Drosophila larvae increased and the comet assay indicated significantly more DNA damage in larvae exposed to high Cd concentrations. The nitroblue tetrazolium (NBT) experiments proved that content of reactive oxygen species (ROS) increased, which indicated Cd exposure could induce oxidative stress. In addition, the expression of mitochondrial adenine nucleotide transferase coding gene (sesB and Ant2) and apoptosis related genes (Debcl, hid, rpr, p53, Sce and Diap1) changed, which may lead to increased apoptosis. These findings confirmed the toxicity effects on oxidative stress and cell apoptosis in Drosophila larvae after early cadmium exposure, providing insights into understanding the effects of heavy metal stress in animal development. Nature Publishing Group UK 2022-03-19 /pmc/articles/PMC8934349/ /pubmed/35307728 http://dx.doi.org/10.1038/s41598-022-08758-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yang, Pingping Yang, Xingran Sun, Liran Han, Xiaobing Xu, Lu Gu, Wei Zhang, Min Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae |
title | Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae |
title_full | Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae |
title_fullStr | Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae |
title_full_unstemmed | Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae |
title_short | Effects of cadmium on oxidative stress and cell apoptosis in Drosophila melanogaster larvae |
title_sort | effects of cadmium on oxidative stress and cell apoptosis in drosophila melanogaster larvae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934349/ https://www.ncbi.nlm.nih.gov/pubmed/35307728 http://dx.doi.org/10.1038/s41598-022-08758-0 |
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