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Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice

We examined whether exercise is associated with hippocampus-mediated improvement in insulin signaling and cell differentiation in the triple transgenic mouse model of Alzheimer disease (3xTg AD) murine model following exposure to 40-Hz light flickering and exercise. We subjected 12-month-old 3xTg AD...

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Autores principales: Kim, Seong-Hyun, Park, Sang-Seo, Kim, Chang-Ju, Kim, Tae-Woon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Exercise Rehabilitation 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934612/
https://www.ncbi.nlm.nih.gov/pubmed/35356135
http://dx.doi.org/10.12965/jer.2244042.021
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author Kim, Seong-Hyun
Park, Sang-Seo
Kim, Chang-Ju
Kim, Tae-Woon
author_facet Kim, Seong-Hyun
Park, Sang-Seo
Kim, Chang-Ju
Kim, Tae-Woon
author_sort Kim, Seong-Hyun
collection PubMed
description We examined whether exercise is associated with hippocampus-mediated improvement in insulin signaling and cell differentiation in the triple transgenic mouse model of Alzheimer disease (3xTg AD) murine model following exposure to 40-Hz light flickering and exercise. We subjected 12-month-old 3xTg AD mice to exercise and 40-Hz light flickering for 3 months. The exercise session was proceeded for 12 consecutive weeks with gradual increase of intensity. To investigate insulin signaling proteins, western blot was conducted to detect the ratio of phosphorylated insulin receptor β (p-IRβ)/total IRβ (t-IRβ), phosphorylated insulin receptor substrate 1 (p-IRS-1)/total IRS-1 (t-IRS-1), phosphorylated phosphatidylinositide-3-kinase (p-PI3K)/total PI3K (t-PI3K), phosphorylated 3-phosphoinositide dependent protein kinase-1 (p-PDK1)/total PDK-1 (t-PDK1), phosphorylated protein kinase B (p-Akt)/total-Akt (t-Akt), and phosphorylated glycogen synthase kinase 3 beta (p-GSK3β)/total GSK3β (t-GSK3β). Doublecortin immunohistochemistry was performed for assessing cell differentiation in the hippocampus. Treatments exerted a positive effect. The combination of exercise and 40-Hz light flickering exposure was the most effective treatment enhancing insulin signaling. Increased ratio of p-IRβ/t-IRβ, p-IRS-1/t-IRS-1, p-PI3K/t-PI3K, p-PDK1/t-PDK1, p-Akt/t-Akt, and p-GSK3β/t-GSK3β and enhanced cell differentiation were observed in the 3xTg AD with exercise under 40-Hz light flickering group. Our results indicate that exercise under 40-Hz light flickering most potently improved insulin signaling, thereby promoted cell differentiation.
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spelling pubmed-89346122022-03-29 Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice Kim, Seong-Hyun Park, Sang-Seo Kim, Chang-Ju Kim, Tae-Woon J Exerc Rehabil Original Article We examined whether exercise is associated with hippocampus-mediated improvement in insulin signaling and cell differentiation in the triple transgenic mouse model of Alzheimer disease (3xTg AD) murine model following exposure to 40-Hz light flickering and exercise. We subjected 12-month-old 3xTg AD mice to exercise and 40-Hz light flickering for 3 months. The exercise session was proceeded for 12 consecutive weeks with gradual increase of intensity. To investigate insulin signaling proteins, western blot was conducted to detect the ratio of phosphorylated insulin receptor β (p-IRβ)/total IRβ (t-IRβ), phosphorylated insulin receptor substrate 1 (p-IRS-1)/total IRS-1 (t-IRS-1), phosphorylated phosphatidylinositide-3-kinase (p-PI3K)/total PI3K (t-PI3K), phosphorylated 3-phosphoinositide dependent protein kinase-1 (p-PDK1)/total PDK-1 (t-PDK1), phosphorylated protein kinase B (p-Akt)/total-Akt (t-Akt), and phosphorylated glycogen synthase kinase 3 beta (p-GSK3β)/total GSK3β (t-GSK3β). Doublecortin immunohistochemistry was performed for assessing cell differentiation in the hippocampus. Treatments exerted a positive effect. The combination of exercise and 40-Hz light flickering exposure was the most effective treatment enhancing insulin signaling. Increased ratio of p-IRβ/t-IRβ, p-IRS-1/t-IRS-1, p-PI3K/t-PI3K, p-PDK1/t-PDK1, p-Akt/t-Akt, and p-GSK3β/t-GSK3β and enhanced cell differentiation were observed in the 3xTg AD with exercise under 40-Hz light flickering group. Our results indicate that exercise under 40-Hz light flickering most potently improved insulin signaling, thereby promoted cell differentiation. Korean Society of Exercise Rehabilitation 2022-02-24 /pmc/articles/PMC8934612/ /pubmed/35356135 http://dx.doi.org/10.12965/jer.2244042.021 Text en Copyright © 2022 Korean Society of Exercise Rehabilitation https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Seong-Hyun
Park, Sang-Seo
Kim, Chang-Ju
Kim, Tae-Woon
Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice
title Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice
title_full Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice
title_fullStr Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice
title_full_unstemmed Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice
title_short Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice
title_sort exercise with 40-hz light flicker improves hippocampal insulin signaling in alzheimer disease mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934612/
https://www.ncbi.nlm.nih.gov/pubmed/35356135
http://dx.doi.org/10.12965/jer.2244042.021
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