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Intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization
Opticin is an extracellular glycoprotein present in the vitreous. Its antiangiogenic properties offer the potential for therapeutic intervention in conditions such as proliferative diabetic retinopathy and retinopathy of prematurity. Here, we investigated the hypothesis that intravitreal administrat...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935380/ https://www.ncbi.nlm.nih.gov/pubmed/34954204 http://dx.doi.org/10.1016/j.exer.2021.108908 |
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author | Klaska, Izabela P. White, Anne Villacampa, Pilar Hoke, Justin Abelleira-Hervas, Laura Maswood, Ryea N. Ali, Robin R. Bunce, Catey Unwin, Richard D. Cooper, Garth J.S. Bishop, Paul N. Bainbridge, James W. |
author_facet | Klaska, Izabela P. White, Anne Villacampa, Pilar Hoke, Justin Abelleira-Hervas, Laura Maswood, Ryea N. Ali, Robin R. Bunce, Catey Unwin, Richard D. Cooper, Garth J.S. Bishop, Paul N. Bainbridge, James W. |
author_sort | Klaska, Izabela P. |
collection | PubMed |
description | Opticin is an extracellular glycoprotein present in the vitreous. Its antiangiogenic properties offer the potential for therapeutic intervention in conditions such as proliferative diabetic retinopathy and retinopathy of prematurity. Here, we investigated the hypothesis that intravitreal administration of recombinant human opticin can safely protect against the development of pathological angiogenesis and promote its regression. We generated and purified recombinant human opticin and investigated its impact on the development and regression of pathological retinal neovascularization following intravitreal administration in murine oxygen-induced retinopathy. We also investigated its effect on normal retinal vascular development and function, following intravitreal injection in neonatal mice, by histological examination and electroretinography. In oxygen-induced retinopathy, intravitreal administration of human recombinant opticin protected against the development of retinal neovascularization to similar extent as aflibercept, which targets VEGF. Opticin also accelerated regression of established retinal neovascularization, though the effect at 18 h was less than that of aflibercept. Intravitreal administration of human recombinant opticin in neonatal mice caused no detectable perturbation of subsequent retinal vascular development or function. In summary we found that intraocular administration of recombinant human opticin protects against the development of pathological angiogenesis in mice and promotes its regression. |
format | Online Article Text |
id | pubmed-8935380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-89353802022-03-24 Intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization Klaska, Izabela P. White, Anne Villacampa, Pilar Hoke, Justin Abelleira-Hervas, Laura Maswood, Ryea N. Ali, Robin R. Bunce, Catey Unwin, Richard D. Cooper, Garth J.S. Bishop, Paul N. Bainbridge, James W. Exp Eye Res Article Opticin is an extracellular glycoprotein present in the vitreous. Its antiangiogenic properties offer the potential for therapeutic intervention in conditions such as proliferative diabetic retinopathy and retinopathy of prematurity. Here, we investigated the hypothesis that intravitreal administration of recombinant human opticin can safely protect against the development of pathological angiogenesis and promote its regression. We generated and purified recombinant human opticin and investigated its impact on the development and regression of pathological retinal neovascularization following intravitreal administration in murine oxygen-induced retinopathy. We also investigated its effect on normal retinal vascular development and function, following intravitreal injection in neonatal mice, by histological examination and electroretinography. In oxygen-induced retinopathy, intravitreal administration of human recombinant opticin protected against the development of retinal neovascularization to similar extent as aflibercept, which targets VEGF. Opticin also accelerated regression of established retinal neovascularization, though the effect at 18 h was less than that of aflibercept. Intravitreal administration of human recombinant opticin in neonatal mice caused no detectable perturbation of subsequent retinal vascular development or function. In summary we found that intraocular administration of recombinant human opticin protects against the development of pathological angiogenesis in mice and promotes its regression. Academic Press 2022-02 /pmc/articles/PMC8935380/ /pubmed/34954204 http://dx.doi.org/10.1016/j.exer.2021.108908 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Klaska, Izabela P. White, Anne Villacampa, Pilar Hoke, Justin Abelleira-Hervas, Laura Maswood, Ryea N. Ali, Robin R. Bunce, Catey Unwin, Richard D. Cooper, Garth J.S. Bishop, Paul N. Bainbridge, James W. Intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization |
title | Intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization |
title_full | Intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization |
title_fullStr | Intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization |
title_full_unstemmed | Intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization |
title_short | Intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization |
title_sort | intravitreal administration of recombinant human opticin protects against hyperoxia-induced pre-retinal neovascularization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935380/ https://www.ncbi.nlm.nih.gov/pubmed/34954204 http://dx.doi.org/10.1016/j.exer.2021.108908 |
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