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Peptide-based simple detection of SARS-CoV-2 with electrochemical readout
Coronavirus disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is considered one of the worst pandemic outbreaks worldwide. This ongoing pandemic urgently requires rapid, accurate, and specific testing devices to detect the virus. We report a simple electr...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935448/ https://www.ncbi.nlm.nih.gov/pubmed/35414399 http://dx.doi.org/10.1016/j.aca.2022.339739 |
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author | Soto, Dayana Orozco, Jahir |
author_facet | Soto, Dayana Orozco, Jahir |
author_sort | Soto, Dayana |
collection | PubMed |
description | Coronavirus disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is considered one of the worst pandemic outbreaks worldwide. This ongoing pandemic urgently requires rapid, accurate, and specific testing devices to detect the virus. We report a simple electrochemical biosensor based on a highly specific synthetic peptide to detect SARS-CoV-2 Spike protein. Unlike other reported electrochemical biosensors involving nanomaterials or complex approaches, our electrochemical platform uses screen-printed gold electrodes functionalized with the thiolated peptide, whose interaction with the Spike protein is directly followed by Electrochemical Impedance Spectroscopy. The electrochemical platform was Spike protein concentration-dependent, with high sensitivity and reproducibility and a limit of detection of 18.2 ng/mL when tested in Spike protein commercial solutions and 0.01 copies/mL in lysed SARS-CoV-2 particles. The label-free biosensor successfully detected the Spike protein in samples from infected patients straightforwardly in only 15 min. The simplicity of the proposed format combined with an on-demand designed peptide opens the path for detecting other pathogen-related antigens. |
format | Online Article Text |
id | pubmed-8935448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89354482022-03-21 Peptide-based simple detection of SARS-CoV-2 with electrochemical readout Soto, Dayana Orozco, Jahir Anal Chim Acta Article Coronavirus disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is considered one of the worst pandemic outbreaks worldwide. This ongoing pandemic urgently requires rapid, accurate, and specific testing devices to detect the virus. We report a simple electrochemical biosensor based on a highly specific synthetic peptide to detect SARS-CoV-2 Spike protein. Unlike other reported electrochemical biosensors involving nanomaterials or complex approaches, our electrochemical platform uses screen-printed gold electrodes functionalized with the thiolated peptide, whose interaction with the Spike protein is directly followed by Electrochemical Impedance Spectroscopy. The electrochemical platform was Spike protein concentration-dependent, with high sensitivity and reproducibility and a limit of detection of 18.2 ng/mL when tested in Spike protein commercial solutions and 0.01 copies/mL in lysed SARS-CoV-2 particles. The label-free biosensor successfully detected the Spike protein in samples from infected patients straightforwardly in only 15 min. The simplicity of the proposed format combined with an on-demand designed peptide opens the path for detecting other pathogen-related antigens. Elsevier B.V. 2022-05-01 2022-03-21 /pmc/articles/PMC8935448/ /pubmed/35414399 http://dx.doi.org/10.1016/j.aca.2022.339739 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Soto, Dayana Orozco, Jahir Peptide-based simple detection of SARS-CoV-2 with electrochemical readout |
title | Peptide-based simple detection of SARS-CoV-2 with electrochemical readout |
title_full | Peptide-based simple detection of SARS-CoV-2 with electrochemical readout |
title_fullStr | Peptide-based simple detection of SARS-CoV-2 with electrochemical readout |
title_full_unstemmed | Peptide-based simple detection of SARS-CoV-2 with electrochemical readout |
title_short | Peptide-based simple detection of SARS-CoV-2 with electrochemical readout |
title_sort | peptide-based simple detection of sars-cov-2 with electrochemical readout |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935448/ https://www.ncbi.nlm.nih.gov/pubmed/35414399 http://dx.doi.org/10.1016/j.aca.2022.339739 |
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