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Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells
BACKGROUND: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increa...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935628/ https://www.ncbi.nlm.nih.gov/pubmed/35321027 http://dx.doi.org/10.2147/IJN.S352349 |
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author | Sztandera, Krzysztof Gorzkiewicz, Michał Bątal, Mateusz Arkhipova, Valeria Knauer, Nadezhda Sánchez-Nieves, Javier de la Mata, Fco Javier Gómez, Rafael Apartsin, Evgeny Klajnert-Maculewicz, Barbara |
author_facet | Sztandera, Krzysztof Gorzkiewicz, Michał Bątal, Mateusz Arkhipova, Valeria Knauer, Nadezhda Sánchez-Nieves, Javier de la Mata, Fco Javier Gómez, Rafael Apartsin, Evgeny Klajnert-Maculewicz, Barbara |
author_sort | Sztandera, Krzysztof |
collection | PubMed |
description | BACKGROUND: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem. METHODS: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H(2)DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy). RESULTS: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer. CONCLUSION: Triazine-carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy. |
format | Online Article Text |
id | pubmed-8935628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-89356282022-03-22 Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells Sztandera, Krzysztof Gorzkiewicz, Michał Bątal, Mateusz Arkhipova, Valeria Knauer, Nadezhda Sánchez-Nieves, Javier de la Mata, Fco Javier Gómez, Rafael Apartsin, Evgeny Klajnert-Maculewicz, Barbara Int J Nanomedicine Original Research BACKGROUND: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem. METHODS: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H(2)DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy). RESULTS: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer. CONCLUSION: Triazine-carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy. Dove 2022-03-15 /pmc/articles/PMC8935628/ /pubmed/35321027 http://dx.doi.org/10.2147/IJN.S352349 Text en © 2022 Sztandera et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Sztandera, Krzysztof Gorzkiewicz, Michał Bątal, Mateusz Arkhipova, Valeria Knauer, Nadezhda Sánchez-Nieves, Javier de la Mata, Fco Javier Gómez, Rafael Apartsin, Evgeny Klajnert-Maculewicz, Barbara Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells |
title | Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells |
title_full | Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells |
title_fullStr | Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells |
title_full_unstemmed | Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells |
title_short | Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells |
title_sort | triazine–carbosilane dendrimersomes enhance cellular uptake and phototoxic activity of rose bengal in basal cell skin carcinoma cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935628/ https://www.ncbi.nlm.nih.gov/pubmed/35321027 http://dx.doi.org/10.2147/IJN.S352349 |
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