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Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells

BACKGROUND: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increa...

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Autores principales: Sztandera, Krzysztof, Gorzkiewicz, Michał, Bątal, Mateusz, Arkhipova, Valeria, Knauer, Nadezhda, Sánchez-Nieves, Javier, de la Mata, Fco Javier, Gómez, Rafael, Apartsin, Evgeny, Klajnert-Maculewicz, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935628/
https://www.ncbi.nlm.nih.gov/pubmed/35321027
http://dx.doi.org/10.2147/IJN.S352349
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author Sztandera, Krzysztof
Gorzkiewicz, Michał
Bątal, Mateusz
Arkhipova, Valeria
Knauer, Nadezhda
Sánchez-Nieves, Javier
de la Mata, Fco Javier
Gómez, Rafael
Apartsin, Evgeny
Klajnert-Maculewicz, Barbara
author_facet Sztandera, Krzysztof
Gorzkiewicz, Michał
Bątal, Mateusz
Arkhipova, Valeria
Knauer, Nadezhda
Sánchez-Nieves, Javier
de la Mata, Fco Javier
Gómez, Rafael
Apartsin, Evgeny
Klajnert-Maculewicz, Barbara
author_sort Sztandera, Krzysztof
collection PubMed
description BACKGROUND: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem. METHODS: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H(2)DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy). RESULTS: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer. CONCLUSION: Triazine-carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy.
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spelling pubmed-89356282022-03-22 Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells Sztandera, Krzysztof Gorzkiewicz, Michał Bątal, Mateusz Arkhipova, Valeria Knauer, Nadezhda Sánchez-Nieves, Javier de la Mata, Fco Javier Gómez, Rafael Apartsin, Evgeny Klajnert-Maculewicz, Barbara Int J Nanomedicine Original Research BACKGROUND: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem. METHODS: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H(2)DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy). RESULTS: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer. CONCLUSION: Triazine-carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy. Dove 2022-03-15 /pmc/articles/PMC8935628/ /pubmed/35321027 http://dx.doi.org/10.2147/IJN.S352349 Text en © 2022 Sztandera et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Sztandera, Krzysztof
Gorzkiewicz, Michał
Bątal, Mateusz
Arkhipova, Valeria
Knauer, Nadezhda
Sánchez-Nieves, Javier
de la Mata, Fco Javier
Gómez, Rafael
Apartsin, Evgeny
Klajnert-Maculewicz, Barbara
Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells
title Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells
title_full Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells
title_fullStr Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells
title_full_unstemmed Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells
title_short Triazine–Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells
title_sort triazine–carbosilane dendrimersomes enhance cellular uptake and phototoxic activity of rose bengal in basal cell skin carcinoma cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935628/
https://www.ncbi.nlm.nih.gov/pubmed/35321027
http://dx.doi.org/10.2147/IJN.S352349
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