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Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities

Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-C...

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Autores principales: Li, Lin, Uppal, Timsy, Hartley, Paul D., Gorzalski, Andrew, Pandori, Mark, Picker, Michael A., Verma, Subhash C., Pagilla, Krishna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936115/
https://www.ncbi.nlm.nih.gov/pubmed/35313589
http://dx.doi.org/10.21203/rs.3.rs-1435729/v1
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author Li, Lin
Uppal, Timsy
Hartley, Paul D.
Gorzalski, Andrew
Pandori, Mark
Picker, Michael A.
Verma, Subhash C.
Pagilla, Krishna
author_facet Li, Lin
Uppal, Timsy
Hartley, Paul D.
Gorzalski, Andrew
Pandori, Mark
Picker, Michael A.
Verma, Subhash C.
Pagilla, Krishna
author_sort Li, Lin
collection PubMed
description Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-CoV-2 variants, including those being monitored for having enhanced transmissibility. We sequenced genomic RNA derived from wastewater to determine the variants of coronaviruses circulating in the communities. Wastewater samples were collected from Truckee Meadows Water Reclamation Facility (TMWRF) from November 2021 to June 2021 were analyzed for SARS-CoV-2 variants and were compared with the variants detected in the clinical specimens (nasal/nasopharyngeal swabs) of infected individuals during the same period. The comparison was found to be conclusively in agreement. Therefore, wastewater monitoring for SARS-CoV-2 variants in the community is a feasible strategy both as a complementary tool to clinical specimen testing and in the latter’s absence.
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spelling pubmed-89361152022-03-22 Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities Li, Lin Uppal, Timsy Hartley, Paul D. Gorzalski, Andrew Pandori, Mark Picker, Michael A. Verma, Subhash C. Pagilla, Krishna Res Sq Article Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-CoV-2 variants, including those being monitored for having enhanced transmissibility. We sequenced genomic RNA derived from wastewater to determine the variants of coronaviruses circulating in the communities. Wastewater samples were collected from Truckee Meadows Water Reclamation Facility (TMWRF) from November 2021 to June 2021 were analyzed for SARS-CoV-2 variants and were compared with the variants detected in the clinical specimens (nasal/nasopharyngeal swabs) of infected individuals during the same period. The comparison was found to be conclusively in agreement. Therefore, wastewater monitoring for SARS-CoV-2 variants in the community is a feasible strategy both as a complementary tool to clinical specimen testing and in the latter’s absence. American Journal Experts 2022-03-17 /pmc/articles/PMC8936115/ /pubmed/35313589 http://dx.doi.org/10.21203/rs.3.rs-1435729/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Li, Lin
Uppal, Timsy
Hartley, Paul D.
Gorzalski, Andrew
Pandori, Mark
Picker, Michael A.
Verma, Subhash C.
Pagilla, Krishna
Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities
title Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities
title_full Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities
title_fullStr Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities
title_full_unstemmed Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities
title_short Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities
title_sort detecting sars-cov-2 variants in wastewater and their correlation with circulating variants in the communities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936115/
https://www.ncbi.nlm.nih.gov/pubmed/35313589
http://dx.doi.org/10.21203/rs.3.rs-1435729/v1
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