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Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities
Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-C...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Journal Experts
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936115/ https://www.ncbi.nlm.nih.gov/pubmed/35313589 http://dx.doi.org/10.21203/rs.3.rs-1435729/v1 |
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author | Li, Lin Uppal, Timsy Hartley, Paul D. Gorzalski, Andrew Pandori, Mark Picker, Michael A. Verma, Subhash C. Pagilla, Krishna |
author_facet | Li, Lin Uppal, Timsy Hartley, Paul D. Gorzalski, Andrew Pandori, Mark Picker, Michael A. Verma, Subhash C. Pagilla, Krishna |
author_sort | Li, Lin |
collection | PubMed |
description | Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-CoV-2 variants, including those being monitored for having enhanced transmissibility. We sequenced genomic RNA derived from wastewater to determine the variants of coronaviruses circulating in the communities. Wastewater samples were collected from Truckee Meadows Water Reclamation Facility (TMWRF) from November 2021 to June 2021 were analyzed for SARS-CoV-2 variants and were compared with the variants detected in the clinical specimens (nasal/nasopharyngeal swabs) of infected individuals during the same period. The comparison was found to be conclusively in agreement. Therefore, wastewater monitoring for SARS-CoV-2 variants in the community is a feasible strategy both as a complementary tool to clinical specimen testing and in the latter’s absence. |
format | Online Article Text |
id | pubmed-8936115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Journal Experts |
record_format | MEDLINE/PubMed |
spelling | pubmed-89361152022-03-22 Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities Li, Lin Uppal, Timsy Hartley, Paul D. Gorzalski, Andrew Pandori, Mark Picker, Michael A. Verma, Subhash C. Pagilla, Krishna Res Sq Article Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-CoV-2 variants, including those being monitored for having enhanced transmissibility. We sequenced genomic RNA derived from wastewater to determine the variants of coronaviruses circulating in the communities. Wastewater samples were collected from Truckee Meadows Water Reclamation Facility (TMWRF) from November 2021 to June 2021 were analyzed for SARS-CoV-2 variants and were compared with the variants detected in the clinical specimens (nasal/nasopharyngeal swabs) of infected individuals during the same period. The comparison was found to be conclusively in agreement. Therefore, wastewater monitoring for SARS-CoV-2 variants in the community is a feasible strategy both as a complementary tool to clinical specimen testing and in the latter’s absence. American Journal Experts 2022-03-17 /pmc/articles/PMC8936115/ /pubmed/35313589 http://dx.doi.org/10.21203/rs.3.rs-1435729/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Li, Lin Uppal, Timsy Hartley, Paul D. Gorzalski, Andrew Pandori, Mark Picker, Michael A. Verma, Subhash C. Pagilla, Krishna Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities |
title | Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities |
title_full | Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities |
title_fullStr | Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities |
title_full_unstemmed | Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities |
title_short | Detecting SARS-CoV-2 Variants in Wastewater and Their Correlation With Circulating Variants in the Communities |
title_sort | detecting sars-cov-2 variants in wastewater and their correlation with circulating variants in the communities |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936115/ https://www.ncbi.nlm.nih.gov/pubmed/35313589 http://dx.doi.org/10.21203/rs.3.rs-1435729/v1 |
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