Cargando…

Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES

A new methodology for the selective extraction of antioxidants from mate tea leaves (and decaffeinated mate tea leaves), using different natural deep eutectic systems (NADES), is reported in this paper. A fractionated extraction was carried out and the optimization of the extraction conditions such...

Descripción completa

Detalles Bibliográficos
Autores principales: Rebocho, Sílvia, Mano, Francisca, Cassel, Eduardo, Anacleto, Beatriz, Bronze, Maria do Rosário, Paiva, Alexandre, Duarte, Ana Rita C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938280/
https://www.ncbi.nlm.nih.gov/pubmed/35330867
http://dx.doi.org/10.1016/j.crfs.2022.03.004
_version_ 1784672520650096640
author Rebocho, Sílvia
Mano, Francisca
Cassel, Eduardo
Anacleto, Beatriz
Bronze, Maria do Rosário
Paiva, Alexandre
Duarte, Ana Rita C.
author_facet Rebocho, Sílvia
Mano, Francisca
Cassel, Eduardo
Anacleto, Beatriz
Bronze, Maria do Rosário
Paiva, Alexandre
Duarte, Ana Rita C.
author_sort Rebocho, Sílvia
collection PubMed
description A new methodology for the selective extraction of antioxidants from mate tea leaves (and decaffeinated mate tea leaves), using different natural deep eutectic systems (NADES), is reported in this paper. A fractionated extraction was carried out and the optimization of the extraction conditions such as solid/liquid ratio, temperature, time, stirring and the use of ultrasound assisted extraction (UAE) technology was performed. The results demonstrate that a sequential extraction using, in a first step, an hydrophobic system Men:Lau (2:1) and, in a second step, an hydrophilic lactic acid-based NADES, leads to two distinct extracts: the first one rich in pigments and the second one rich in polyphenols. NADES systems were able to extract 30% more of the polyphenolic components of the mate tea leaves matrices, when compared with traditional solvents/techniques. Moreover, it has been shown that the incorporation of the extract in the NADES, compared to the same extract in aqueous medium was beneficial for the stabilization of the antioxidants. It maintains their functionality at least for three months, reaching 41% more versus the extracts obtained by traditional solvents/techniques. The absence of caffeine in the extracts did not shown to have any effects on the stability results.
format Online
Article
Text
id pubmed-8938280
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-89382802022-03-23 Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES Rebocho, Sílvia Mano, Francisca Cassel, Eduardo Anacleto, Beatriz Bronze, Maria do Rosário Paiva, Alexandre Duarte, Ana Rita C. Curr Res Food Sci Research Article A new methodology for the selective extraction of antioxidants from mate tea leaves (and decaffeinated mate tea leaves), using different natural deep eutectic systems (NADES), is reported in this paper. A fractionated extraction was carried out and the optimization of the extraction conditions such as solid/liquid ratio, temperature, time, stirring and the use of ultrasound assisted extraction (UAE) technology was performed. The results demonstrate that a sequential extraction using, in a first step, an hydrophobic system Men:Lau (2:1) and, in a second step, an hydrophilic lactic acid-based NADES, leads to two distinct extracts: the first one rich in pigments and the second one rich in polyphenols. NADES systems were able to extract 30% more of the polyphenolic components of the mate tea leaves matrices, when compared with traditional solvents/techniques. Moreover, it has been shown that the incorporation of the extract in the NADES, compared to the same extract in aqueous medium was beneficial for the stabilization of the antioxidants. It maintains their functionality at least for three months, reaching 41% more versus the extracts obtained by traditional solvents/techniques. The absence of caffeine in the extracts did not shown to have any effects on the stability results. Elsevier 2022-03-15 /pmc/articles/PMC8938280/ /pubmed/35330867 http://dx.doi.org/10.1016/j.crfs.2022.03.004 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Rebocho, Sílvia
Mano, Francisca
Cassel, Eduardo
Anacleto, Beatriz
Bronze, Maria do Rosário
Paiva, Alexandre
Duarte, Ana Rita C.
Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES
title Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES
title_full Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES
title_fullStr Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES
title_full_unstemmed Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES
title_short Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES
title_sort fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic nades
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938280/
https://www.ncbi.nlm.nih.gov/pubmed/35330867
http://dx.doi.org/10.1016/j.crfs.2022.03.004
work_keys_str_mv AT rebochosilvia fractionatedextractionofpolyphenolsfrommatetealeavesusingacombinationofhydrophobichydrophilicnades
AT manofrancisca fractionatedextractionofpolyphenolsfrommatetealeavesusingacombinationofhydrophobichydrophilicnades
AT casseleduardo fractionatedextractionofpolyphenolsfrommatetealeavesusingacombinationofhydrophobichydrophilicnades
AT anacletobeatriz fractionatedextractionofpolyphenolsfrommatetealeavesusingacombinationofhydrophobichydrophilicnades
AT bronzemariadorosario fractionatedextractionofpolyphenolsfrommatetealeavesusingacombinationofhydrophobichydrophilicnades
AT paivaalexandre fractionatedextractionofpolyphenolsfrommatetealeavesusingacombinationofhydrophobichydrophilicnades
AT duarteanaritac fractionatedextractionofpolyphenolsfrommatetealeavesusingacombinationofhydrophobichydrophilicnades