Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats

CRISPR/Cas has been mainly used for mutagenesis through the induction of double strand breaks (DSBs) within unique protein-coding genes. Using the SaCas9 nuclease to induce multiple DSBs in functional repetitive DNA of Arabidopsis thaliana, we can now show that cell death can be induced in a control...

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Autores principales: Schindele, Angelina, Gehrke, Fabienne, Schmidt, Carla, Röhrig, Sarah, Dorn, Annika, Puchta, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938420/
https://www.ncbi.nlm.nih.gov/pubmed/35314679
http://dx.doi.org/10.1038/s41467-022-29130-w
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author Schindele, Angelina
Gehrke, Fabienne
Schmidt, Carla
Röhrig, Sarah
Dorn, Annika
Puchta, Holger
author_facet Schindele, Angelina
Gehrke, Fabienne
Schmidt, Carla
Röhrig, Sarah
Dorn, Annika
Puchta, Holger
author_sort Schindele, Angelina
collection PubMed
description CRISPR/Cas has been mainly used for mutagenesis through the induction of double strand breaks (DSBs) within unique protein-coding genes. Using the SaCas9 nuclease to induce multiple DSBs in functional repetitive DNA of Arabidopsis thaliana, we can now show that cell death can be induced in a controlled way. This approach, named CRISPR-Kill, can be used as tool for tissue engineering. By simply exchanging the constitutive promoter of SaCas9 with cell type-specific promoters, it is possible to block organogenesis in Arabidopsis. By AP1-specific expression of CRISPR-Kill, we are able to restore the apetala1 phenotype and to specifically eliminate petals. In addition, by expressing CRISPR-Kill in root-specific pericycle cells, we are able to dramatically reduce the number and the length of lateral roots. In the future, the application of CRISPR-Kill may not only help to control development but could also be used to change the biochemical properties of plants.
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spelling pubmed-89384202022-04-08 Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats Schindele, Angelina Gehrke, Fabienne Schmidt, Carla Röhrig, Sarah Dorn, Annika Puchta, Holger Nat Commun Article CRISPR/Cas has been mainly used for mutagenesis through the induction of double strand breaks (DSBs) within unique protein-coding genes. Using the SaCas9 nuclease to induce multiple DSBs in functional repetitive DNA of Arabidopsis thaliana, we can now show that cell death can be induced in a controlled way. This approach, named CRISPR-Kill, can be used as tool for tissue engineering. By simply exchanging the constitutive promoter of SaCas9 with cell type-specific promoters, it is possible to block organogenesis in Arabidopsis. By AP1-specific expression of CRISPR-Kill, we are able to restore the apetala1 phenotype and to specifically eliminate petals. In addition, by expressing CRISPR-Kill in root-specific pericycle cells, we are able to dramatically reduce the number and the length of lateral roots. In the future, the application of CRISPR-Kill may not only help to control development but could also be used to change the biochemical properties of plants. Nature Publishing Group UK 2022-03-21 /pmc/articles/PMC8938420/ /pubmed/35314679 http://dx.doi.org/10.1038/s41467-022-29130-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Schindele, Angelina
Gehrke, Fabienne
Schmidt, Carla
Röhrig, Sarah
Dorn, Annika
Puchta, Holger
Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
title Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
title_full Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
title_fullStr Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
title_full_unstemmed Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
title_short Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
title_sort using crispr-kill for organ specific cell elimination by cleavage of tandem repeats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938420/
https://www.ncbi.nlm.nih.gov/pubmed/35314679
http://dx.doi.org/10.1038/s41467-022-29130-w
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