Cargando…
Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples
Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigue’ and reduced confidence in test results. Consequ...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938789/ https://www.ncbi.nlm.nih.gov/pubmed/35314662 http://dx.doi.org/10.3390/mps5020025 |
_version_ | 1784672622506672128 |
---|---|
author | Tarantini, Francesco Saverio Wu, Siyu Jenkins, Harry Tellechea Lopez, Ana Tomlin, Hannah Hyde, Ralph Lis-Slimak, Katarzyna Thompson, Jamie Louise Pijuan-Galitó, Sara Scales, Danielle Kaneko, Kazuyo Dey, Jayasree Park, Emily Hill, Jack Lee, I-Ning Doolan, Lara Arendt-Tranholm, Asta Denning, Chris Seedhouse, Claire Benest, Andrew V. |
author_facet | Tarantini, Francesco Saverio Wu, Siyu Jenkins, Harry Tellechea Lopez, Ana Tomlin, Hannah Hyde, Ralph Lis-Slimak, Katarzyna Thompson, Jamie Louise Pijuan-Galitó, Sara Scales, Danielle Kaneko, Kazuyo Dey, Jayasree Park, Emily Hill, Jack Lee, I-Ning Doolan, Lara Arendt-Tranholm, Asta Denning, Chris Seedhouse, Claire Benest, Andrew V. |
author_sort | Tarantini, Francesco Saverio |
collection | PubMed |
description | Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigue’ and reduced confidence in test results. Consequently, asymptomatic individuals have developed reluctance towards repeat testing, which remains the best way to monitor COVID-19 cases in the wider population. On the other hand, saliva-based PCR, a non-invasive, highly sensitive, and accurate test suitable for everyone, is gaining momentum as a straightforward and reliable means of detecting SARS-CoV-2 in symptomatic and asymptomatic individuals. Here, we provide an itemised list of the equipment and reagents involved in the process of sample submission, inactivation and analysis, as well as a detailed description of how each of these steps is performed. |
format | Online Article Text |
id | pubmed-8938789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89387892022-03-23 Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples Tarantini, Francesco Saverio Wu, Siyu Jenkins, Harry Tellechea Lopez, Ana Tomlin, Hannah Hyde, Ralph Lis-Slimak, Katarzyna Thompson, Jamie Louise Pijuan-Galitó, Sara Scales, Danielle Kaneko, Kazuyo Dey, Jayasree Park, Emily Hill, Jack Lee, I-Ning Doolan, Lara Arendt-Tranholm, Asta Denning, Chris Seedhouse, Claire Benest, Andrew V. Methods Protoc Protocol Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigue’ and reduced confidence in test results. Consequently, asymptomatic individuals have developed reluctance towards repeat testing, which remains the best way to monitor COVID-19 cases in the wider population. On the other hand, saliva-based PCR, a non-invasive, highly sensitive, and accurate test suitable for everyone, is gaining momentum as a straightforward and reliable means of detecting SARS-CoV-2 in symptomatic and asymptomatic individuals. Here, we provide an itemised list of the equipment and reagents involved in the process of sample submission, inactivation and analysis, as well as a detailed description of how each of these steps is performed. MDPI 2022-03-07 /pmc/articles/PMC8938789/ /pubmed/35314662 http://dx.doi.org/10.3390/mps5020025 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Tarantini, Francesco Saverio Wu, Siyu Jenkins, Harry Tellechea Lopez, Ana Tomlin, Hannah Hyde, Ralph Lis-Slimak, Katarzyna Thompson, Jamie Louise Pijuan-Galitó, Sara Scales, Danielle Kaneko, Kazuyo Dey, Jayasree Park, Emily Hill, Jack Lee, I-Ning Doolan, Lara Arendt-Tranholm, Asta Denning, Chris Seedhouse, Claire Benest, Andrew V. Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples |
title | Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples |
title_full | Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples |
title_fullStr | Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples |
title_full_unstemmed | Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples |
title_short | Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples |
title_sort | direct rt-qpcr assay for the detection of sars-cov-2 in saliva samples |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938789/ https://www.ncbi.nlm.nih.gov/pubmed/35314662 http://dx.doi.org/10.3390/mps5020025 |
work_keys_str_mv | AT tarantinifrancescosaverio directrtqpcrassayforthedetectionofsarscov2insalivasamples AT wusiyu directrtqpcrassayforthedetectionofsarscov2insalivasamples AT jenkinsharry directrtqpcrassayforthedetectionofsarscov2insalivasamples AT tellechealopezana directrtqpcrassayforthedetectionofsarscov2insalivasamples AT tomlinhannah directrtqpcrassayforthedetectionofsarscov2insalivasamples AT hyderalph directrtqpcrassayforthedetectionofsarscov2insalivasamples AT lisslimakkatarzyna directrtqpcrassayforthedetectionofsarscov2insalivasamples AT thompsonjamielouise directrtqpcrassayforthedetectionofsarscov2insalivasamples AT pijuangalitosara directrtqpcrassayforthedetectionofsarscov2insalivasamples AT scalesdanielle directrtqpcrassayforthedetectionofsarscov2insalivasamples AT kanekokazuyo directrtqpcrassayforthedetectionofsarscov2insalivasamples AT deyjayasree directrtqpcrassayforthedetectionofsarscov2insalivasamples AT parkemily directrtqpcrassayforthedetectionofsarscov2insalivasamples AT hilljack directrtqpcrassayforthedetectionofsarscov2insalivasamples AT leeining directrtqpcrassayforthedetectionofsarscov2insalivasamples AT doolanlara directrtqpcrassayforthedetectionofsarscov2insalivasamples AT arendttranholmasta directrtqpcrassayforthedetectionofsarscov2insalivasamples AT denningchris directrtqpcrassayforthedetectionofsarscov2insalivasamples AT seedhouseclaire directrtqpcrassayforthedetectionofsarscov2insalivasamples AT benestandrewv directrtqpcrassayforthedetectionofsarscov2insalivasamples |