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Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples

Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigue’ and reduced confidence in test results. Consequ...

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Autores principales: Tarantini, Francesco Saverio, Wu, Siyu, Jenkins, Harry, Tellechea Lopez, Ana, Tomlin, Hannah, Hyde, Ralph, Lis-Slimak, Katarzyna, Thompson, Jamie Louise, Pijuan-Galitó, Sara, Scales, Danielle, Kaneko, Kazuyo, Dey, Jayasree, Park, Emily, Hill, Jack, Lee, I-Ning, Doolan, Lara, Arendt-Tranholm, Asta, Denning, Chris, Seedhouse, Claire, Benest, Andrew V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938789/
https://www.ncbi.nlm.nih.gov/pubmed/35314662
http://dx.doi.org/10.3390/mps5020025
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author Tarantini, Francesco Saverio
Wu, Siyu
Jenkins, Harry
Tellechea Lopez, Ana
Tomlin, Hannah
Hyde, Ralph
Lis-Slimak, Katarzyna
Thompson, Jamie Louise
Pijuan-Galitó, Sara
Scales, Danielle
Kaneko, Kazuyo
Dey, Jayasree
Park, Emily
Hill, Jack
Lee, I-Ning
Doolan, Lara
Arendt-Tranholm, Asta
Denning, Chris
Seedhouse, Claire
Benest, Andrew V.
author_facet Tarantini, Francesco Saverio
Wu, Siyu
Jenkins, Harry
Tellechea Lopez, Ana
Tomlin, Hannah
Hyde, Ralph
Lis-Slimak, Katarzyna
Thompson, Jamie Louise
Pijuan-Galitó, Sara
Scales, Danielle
Kaneko, Kazuyo
Dey, Jayasree
Park, Emily
Hill, Jack
Lee, I-Ning
Doolan, Lara
Arendt-Tranholm, Asta
Denning, Chris
Seedhouse, Claire
Benest, Andrew V.
author_sort Tarantini, Francesco Saverio
collection PubMed
description Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigue’ and reduced confidence in test results. Consequently, asymptomatic individuals have developed reluctance towards repeat testing, which remains the best way to monitor COVID-19 cases in the wider population. On the other hand, saliva-based PCR, a non-invasive, highly sensitive, and accurate test suitable for everyone, is gaining momentum as a straightforward and reliable means of detecting SARS-CoV-2 in symptomatic and asymptomatic individuals. Here, we provide an itemised list of the equipment and reagents involved in the process of sample submission, inactivation and analysis, as well as a detailed description of how each of these steps is performed.
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spelling pubmed-89387892022-03-23 Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples Tarantini, Francesco Saverio Wu, Siyu Jenkins, Harry Tellechea Lopez, Ana Tomlin, Hannah Hyde, Ralph Lis-Slimak, Katarzyna Thompson, Jamie Louise Pijuan-Galitó, Sara Scales, Danielle Kaneko, Kazuyo Dey, Jayasree Park, Emily Hill, Jack Lee, I-Ning Doolan, Lara Arendt-Tranholm, Asta Denning, Chris Seedhouse, Claire Benest, Andrew V. Methods Protoc Protocol Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigue’ and reduced confidence in test results. Consequently, asymptomatic individuals have developed reluctance towards repeat testing, which remains the best way to monitor COVID-19 cases in the wider population. On the other hand, saliva-based PCR, a non-invasive, highly sensitive, and accurate test suitable for everyone, is gaining momentum as a straightforward and reliable means of detecting SARS-CoV-2 in symptomatic and asymptomatic individuals. Here, we provide an itemised list of the equipment and reagents involved in the process of sample submission, inactivation and analysis, as well as a detailed description of how each of these steps is performed. MDPI 2022-03-07 /pmc/articles/PMC8938789/ /pubmed/35314662 http://dx.doi.org/10.3390/mps5020025 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Tarantini, Francesco Saverio
Wu, Siyu
Jenkins, Harry
Tellechea Lopez, Ana
Tomlin, Hannah
Hyde, Ralph
Lis-Slimak, Katarzyna
Thompson, Jamie Louise
Pijuan-Galitó, Sara
Scales, Danielle
Kaneko, Kazuyo
Dey, Jayasree
Park, Emily
Hill, Jack
Lee, I-Ning
Doolan, Lara
Arendt-Tranholm, Asta
Denning, Chris
Seedhouse, Claire
Benest, Andrew V.
Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples
title Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples
title_full Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples
title_fullStr Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples
title_full_unstemmed Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples
title_short Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples
title_sort direct rt-qpcr assay for the detection of sars-cov-2 in saliva samples
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938789/
https://www.ncbi.nlm.nih.gov/pubmed/35314662
http://dx.doi.org/10.3390/mps5020025
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