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Dataset for transcriptomic profiles associated with development of sexual structures in Aspergillus flavus

Information on the transcriptomic changes that occur within sclerotia of Aspergillus flavus during its sexual cycle is very limited and warrants further research. The findings will broaden our knowledge of the biology of A. flavus and can provide valuable insights in the development or deployment of...

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Autores principales: Luis, Jane Marian, Carbone, Ignazio, Mack, Brian M., Lebar, Matthew D., Cary, Jeffrey W., Gilbert, Matthew K., Bhatnagar, Deepak, Wientjes, Carol-Carter, Payne, Gary A., Moore, Geromy G., Ameen, Yaken Obaydeh, Ojiambo, Peter S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938862/
https://www.ncbi.nlm.nih.gov/pubmed/35330736
http://dx.doi.org/10.1016/j.dib.2022.108033
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author Luis, Jane Marian
Carbone, Ignazio
Mack, Brian M.
Lebar, Matthew D.
Cary, Jeffrey W.
Gilbert, Matthew K.
Bhatnagar, Deepak
Wientjes, Carol-Carter
Payne, Gary A.
Moore, Geromy G.
Ameen, Yaken Obaydeh
Ojiambo, Peter S.
author_facet Luis, Jane Marian
Carbone, Ignazio
Mack, Brian M.
Lebar, Matthew D.
Cary, Jeffrey W.
Gilbert, Matthew K.
Bhatnagar, Deepak
Wientjes, Carol-Carter
Payne, Gary A.
Moore, Geromy G.
Ameen, Yaken Obaydeh
Ojiambo, Peter S.
author_sort Luis, Jane Marian
collection PubMed
description Information on the transcriptomic changes that occur within sclerotia of Aspergillus flavus during its sexual cycle is very limited and warrants further research. The findings will broaden our knowledge of the biology of A. flavus and can provide valuable insights in the development or deployment of non-toxigenic strains as biocontrol agents against aflatoxigenic strains. This article presents transcriptomic datasets included in our research article entitled, “Development of sexual structures influences metabolomic and transcriptomic profiles in Aspergillus flavus” [1], which utilized transcriptomics to identify possible genes and gene clusters associated with sexual reproduction and fertilization in A. flavus. RNA was extracted from sclerotia of a high fertility cross (Hi-Fert-Mated), a low fertility cross (Lo-Fert-Mated), and unmated strains (Hi-Fert-Unmated and Lo-Fert-Unmated) of A. flavus collected immediately after crossing and at every two weeks until eight weeks of incubation on mixed cereal agar at 30 °C in continuous darkness (n = 4 replicates from each treatment for each time point; 80 total). Raw sequencing reads obtained on an Illumina NovaSeq 6000 were deposited in NCBI's Sequence Read Archive (SRA) repository under BioProject accession number PRJNA789260. Reads were mapped to the A. flavus NRRL 3357 genome (assembly JCVI-afl1-v2.0; GCA_000006275.2) using STAR software. Differential gene expression analyses, functional analyses, and weighted gene co-expression network analysis were performed using DESeq2 R packages. The raw and analyzed data presented in this article could be reused for comparisons with other datasets to obtain transcriptional differences among strains of A. flavus or closely related species. The data can also be used for further investigation of the molecular basis of different processes involved in sexual reproduction and sclerotia fertility in A. flavus.
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spelling pubmed-89388622022-03-23 Dataset for transcriptomic profiles associated with development of sexual structures in Aspergillus flavus Luis, Jane Marian Carbone, Ignazio Mack, Brian M. Lebar, Matthew D. Cary, Jeffrey W. Gilbert, Matthew K. Bhatnagar, Deepak Wientjes, Carol-Carter Payne, Gary A. Moore, Geromy G. Ameen, Yaken Obaydeh Ojiambo, Peter S. Data Brief Data Article Information on the transcriptomic changes that occur within sclerotia of Aspergillus flavus during its sexual cycle is very limited and warrants further research. The findings will broaden our knowledge of the biology of A. flavus and can provide valuable insights in the development or deployment of non-toxigenic strains as biocontrol agents against aflatoxigenic strains. This article presents transcriptomic datasets included in our research article entitled, “Development of sexual structures influences metabolomic and transcriptomic profiles in Aspergillus flavus” [1], which utilized transcriptomics to identify possible genes and gene clusters associated with sexual reproduction and fertilization in A. flavus. RNA was extracted from sclerotia of a high fertility cross (Hi-Fert-Mated), a low fertility cross (Lo-Fert-Mated), and unmated strains (Hi-Fert-Unmated and Lo-Fert-Unmated) of A. flavus collected immediately after crossing and at every two weeks until eight weeks of incubation on mixed cereal agar at 30 °C in continuous darkness (n = 4 replicates from each treatment for each time point; 80 total). Raw sequencing reads obtained on an Illumina NovaSeq 6000 were deposited in NCBI's Sequence Read Archive (SRA) repository under BioProject accession number PRJNA789260. Reads were mapped to the A. flavus NRRL 3357 genome (assembly JCVI-afl1-v2.0; GCA_000006275.2) using STAR software. Differential gene expression analyses, functional analyses, and weighted gene co-expression network analysis were performed using DESeq2 R packages. The raw and analyzed data presented in this article could be reused for comparisons with other datasets to obtain transcriptional differences among strains of A. flavus or closely related species. The data can also be used for further investigation of the molecular basis of different processes involved in sexual reproduction and sclerotia fertility in A. flavus. Elsevier 2022-03-14 /pmc/articles/PMC8938862/ /pubmed/35330736 http://dx.doi.org/10.1016/j.dib.2022.108033 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Luis, Jane Marian
Carbone, Ignazio
Mack, Brian M.
Lebar, Matthew D.
Cary, Jeffrey W.
Gilbert, Matthew K.
Bhatnagar, Deepak
Wientjes, Carol-Carter
Payne, Gary A.
Moore, Geromy G.
Ameen, Yaken Obaydeh
Ojiambo, Peter S.
Dataset for transcriptomic profiles associated with development of sexual structures in Aspergillus flavus
title Dataset for transcriptomic profiles associated with development of sexual structures in Aspergillus flavus
title_full Dataset for transcriptomic profiles associated with development of sexual structures in Aspergillus flavus
title_fullStr Dataset for transcriptomic profiles associated with development of sexual structures in Aspergillus flavus
title_full_unstemmed Dataset for transcriptomic profiles associated with development of sexual structures in Aspergillus flavus
title_short Dataset for transcriptomic profiles associated with development of sexual structures in Aspergillus flavus
title_sort dataset for transcriptomic profiles associated with development of sexual structures in aspergillus flavus
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938862/
https://www.ncbi.nlm.nih.gov/pubmed/35330736
http://dx.doi.org/10.1016/j.dib.2022.108033
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