Protocol for studying microbiome impact on host energy and reproduction in Drosophila

Drosophila gut microbiome in flies has been shown to have a systemic influence on energy production by the host and the energetic investment in growth and reproduction. Here we describe a protocol for studying the mechanisms responsible for this remote regulation by gut bacteria. This protocol enabl...

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Autores principales: Gnainsky, Yulia, Itkin, Maxim, Mehlman, Tevie, Brandis, Alexander, Malitsky, Sergey, Soen, Yoav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938908/
https://www.ncbi.nlm.nih.gov/pubmed/35330965
http://dx.doi.org/10.1016/j.xpro.2022.101253
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author Gnainsky, Yulia
Itkin, Maxim
Mehlman, Tevie
Brandis, Alexander
Malitsky, Sergey
Soen, Yoav
author_facet Gnainsky, Yulia
Itkin, Maxim
Mehlman, Tevie
Brandis, Alexander
Malitsky, Sergey
Soen, Yoav
author_sort Gnainsky, Yulia
collection PubMed
description Drosophila gut microbiome in flies has been shown to have a systemic influence on energy production by the host and the energetic investment in growth and reproduction. Here we describe a protocol for studying the mechanisms responsible for this remote regulation by gut bacteria. This protocol enables whole-body and ovary-specific quantification of energy-storing molecules as well as identification of host metabolites and pathways that are regulated by gut microbiome-derived factors. Similar procedures are applicable to additional treatments and genetic manipulations. For complete details on the use and execution of this protocol, please refer to Gnainsky et al. (2021).
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spelling pubmed-89389082022-03-23 Protocol for studying microbiome impact on host energy and reproduction in Drosophila Gnainsky, Yulia Itkin, Maxim Mehlman, Tevie Brandis, Alexander Malitsky, Sergey Soen, Yoav STAR Protoc Protocol Drosophila gut microbiome in flies has been shown to have a systemic influence on energy production by the host and the energetic investment in growth and reproduction. Here we describe a protocol for studying the mechanisms responsible for this remote regulation by gut bacteria. This protocol enables whole-body and ovary-specific quantification of energy-storing molecules as well as identification of host metabolites and pathways that are regulated by gut microbiome-derived factors. Similar procedures are applicable to additional treatments and genetic manipulations. For complete details on the use and execution of this protocol, please refer to Gnainsky et al. (2021). Elsevier 2022-03-21 /pmc/articles/PMC8938908/ /pubmed/35330965 http://dx.doi.org/10.1016/j.xpro.2022.101253 Text en © 2022. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gnainsky, Yulia
Itkin, Maxim
Mehlman, Tevie
Brandis, Alexander
Malitsky, Sergey
Soen, Yoav
Protocol for studying microbiome impact on host energy and reproduction in Drosophila
title Protocol for studying microbiome impact on host energy and reproduction in Drosophila
title_full Protocol for studying microbiome impact on host energy and reproduction in Drosophila
title_fullStr Protocol for studying microbiome impact on host energy and reproduction in Drosophila
title_full_unstemmed Protocol for studying microbiome impact on host energy and reproduction in Drosophila
title_short Protocol for studying microbiome impact on host energy and reproduction in Drosophila
title_sort protocol for studying microbiome impact on host energy and reproduction in drosophila
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938908/
https://www.ncbi.nlm.nih.gov/pubmed/35330965
http://dx.doi.org/10.1016/j.xpro.2022.101253
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