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Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates

Living materials combine a material scaffold, that is often porous, with engineered cells that perform sensing, computing, and biosynthetic tasks. Designing such systems is difficult because little is known regarding signaling transport parameters in the material. Here, the development of a porous m...

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Autores principales: Vaiana, Christopher A, Kim, Hyungseok, Cottet, Jonathan, Oai, Keiko, Ge, Zhifei, Conforti, Kameron, King, Andrew M, Meyer, Adam J, Chen, Haorong, Voigt, Christopher A, Buie, Cullen R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938921/
https://www.ncbi.nlm.nih.gov/pubmed/35315586
http://dx.doi.org/10.15252/msb.202110785
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author Vaiana, Christopher A
Kim, Hyungseok
Cottet, Jonathan
Oai, Keiko
Ge, Zhifei
Conforti, Kameron
King, Andrew M
Meyer, Adam J
Chen, Haorong
Voigt, Christopher A
Buie, Cullen R
author_facet Vaiana, Christopher A
Kim, Hyungseok
Cottet, Jonathan
Oai, Keiko
Ge, Zhifei
Conforti, Kameron
King, Andrew M
Meyer, Adam J
Chen, Haorong
Voigt, Christopher A
Buie, Cullen R
author_sort Vaiana, Christopher A
collection PubMed
description Living materials combine a material scaffold, that is often porous, with engineered cells that perform sensing, computing, and biosynthetic tasks. Designing such systems is difficult because little is known regarding signaling transport parameters in the material. Here, the development of a porous microplate is presented. Hydrogel barriers between wells have a porosity of 60% and a tortuosity factor of 1.6, allowing molecular diffusion between wells. The permeability of dyes, antibiotics, inducers, and quorum signals between wells were characterized. A “sentinel” strain was constructed by introducing orthogonal sensors into the genome of Escherichia coli MG1655 for IPTG, anhydrotetracycline, L‐arabinose, and four quorum signals. The strain’s response to inducer diffusion through the wells was quantified up to 14 mm, and quorum and antibacterial signaling were measured over 16 h. Signaling distance is dictated by hydrogel adsorption, quantified using a linear finite element model that yields adsorption coefficients from 0 to 0.1 mol m(−3). Parameters derived herein will aid the design of living materials for pathogen remediation, computation, and self‐organizing biofilms.
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spelling pubmed-89389212022-04-07 Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates Vaiana, Christopher A Kim, Hyungseok Cottet, Jonathan Oai, Keiko Ge, Zhifei Conforti, Kameron King, Andrew M Meyer, Adam J Chen, Haorong Voigt, Christopher A Buie, Cullen R Mol Syst Biol Articles Living materials combine a material scaffold, that is often porous, with engineered cells that perform sensing, computing, and biosynthetic tasks. Designing such systems is difficult because little is known regarding signaling transport parameters in the material. Here, the development of a porous microplate is presented. Hydrogel barriers between wells have a porosity of 60% and a tortuosity factor of 1.6, allowing molecular diffusion between wells. The permeability of dyes, antibiotics, inducers, and quorum signals between wells were characterized. A “sentinel” strain was constructed by introducing orthogonal sensors into the genome of Escherichia coli MG1655 for IPTG, anhydrotetracycline, L‐arabinose, and four quorum signals. The strain’s response to inducer diffusion through the wells was quantified up to 14 mm, and quorum and antibacterial signaling were measured over 16 h. Signaling distance is dictated by hydrogel adsorption, quantified using a linear finite element model that yields adsorption coefficients from 0 to 0.1 mol m(−3). Parameters derived herein will aid the design of living materials for pathogen remediation, computation, and self‐organizing biofilms. John Wiley and Sons Inc. 2022-03-22 /pmc/articles/PMC8938921/ /pubmed/35315586 http://dx.doi.org/10.15252/msb.202110785 Text en © 2022 The Authors. Published under the terms of the CC BY 4.0 license https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Vaiana, Christopher A
Kim, Hyungseok
Cottet, Jonathan
Oai, Keiko
Ge, Zhifei
Conforti, Kameron
King, Andrew M
Meyer, Adam J
Chen, Haorong
Voigt, Christopher A
Buie, Cullen R
Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates
title Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates
title_full Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates
title_fullStr Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates
title_full_unstemmed Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates
title_short Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates
title_sort characterizing chemical signaling between engineered “microbial sentinels” in porous microplates
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938921/
https://www.ncbi.nlm.nih.gov/pubmed/35315586
http://dx.doi.org/10.15252/msb.202110785
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