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Impact of angiotensin-converting enzyme inhibition on hemodynamic and autonomic profile of elastase-2 knockout mice
Elastase-2 (ELA-2) is an angiotensin II-generating enzyme that participates in the cardiovascular system. ELA-2 is involved in hemodynamic and autonomic control and is upregulated in myocardial infarction and hypertension. The inhibition of angiotensin-converting enzyme (ACE) increased ELA-2 express...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8939506/ https://www.ncbi.nlm.nih.gov/pubmed/35319673 http://dx.doi.org/10.1590/1414-431X2022e11774 |
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author | Prates-Costa, T.C. de Oliveira, M. Fazan, R. Salgado, H.C. Becari, C. |
author_facet | Prates-Costa, T.C. de Oliveira, M. Fazan, R. Salgado, H.C. Becari, C. |
author_sort | Prates-Costa, T.C. |
collection | PubMed |
description | Elastase-2 (ELA-2) is an angiotensin II-generating enzyme that participates in the cardiovascular system. ELA-2 is involved in hemodynamic and autonomic control and is upregulated in myocardial infarction and hypertension. The inhibition of angiotensin-converting enzyme (ACE) increased ELA-2 expression in the carotid arteries and heart of spontaneously hypertensive rats. In this study, we sought to investigate the role of ACE inhibition in hemodynamic and autonomic balance in elastase-2 knockout (ELA-2 KO) mice. Male ELA-2 KO and C57BL/6 mice were treated with the ACE inhibitor enalapril or saline for 10 days. After treatment, mice underwent surgery for cannulation of the femoral artery and arterial pressure recordings were made five days later in awake animals. The variability of systolic blood pressure (SBP) and pulse interval (PI) was evaluated in the time and frequency domain. Spontaneous baroreflex was assessed by the sequencing method. ACE inhibition caused a significant decrease in mean arterial pressure (117±2.2 vs 100±2.8 mmHg) and an increase in heart rate (570±32 vs 655±15 bpm) in ELA-2 KO mice. Despite a tendency towards reduction in the overall heart rate variability (standard deviation of successive values: 7.6±1.1 vs 4.7±0.6 ms, P=0.08), no changes were found in the root of the mean sum of squares or in the power of the high-frequency band. ACE inhibition did not change the spontaneous baroreflex indices (gain and baroreflex effectiveness index) in ELA-2 KO mice. Altogether, this data suggested that ACE played a role in the maintenance of hemodynamic function in ELA-2 KO mice. |
format | Online Article Text |
id | pubmed-8939506 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-89395062022-04-04 Impact of angiotensin-converting enzyme inhibition on hemodynamic and autonomic profile of elastase-2 knockout mice Prates-Costa, T.C. de Oliveira, M. Fazan, R. Salgado, H.C. Becari, C. Braz J Med Biol Res Research Article Elastase-2 (ELA-2) is an angiotensin II-generating enzyme that participates in the cardiovascular system. ELA-2 is involved in hemodynamic and autonomic control and is upregulated in myocardial infarction and hypertension. The inhibition of angiotensin-converting enzyme (ACE) increased ELA-2 expression in the carotid arteries and heart of spontaneously hypertensive rats. In this study, we sought to investigate the role of ACE inhibition in hemodynamic and autonomic balance in elastase-2 knockout (ELA-2 KO) mice. Male ELA-2 KO and C57BL/6 mice were treated with the ACE inhibitor enalapril or saline for 10 days. After treatment, mice underwent surgery for cannulation of the femoral artery and arterial pressure recordings were made five days later in awake animals. The variability of systolic blood pressure (SBP) and pulse interval (PI) was evaluated in the time and frequency domain. Spontaneous baroreflex was assessed by the sequencing method. ACE inhibition caused a significant decrease in mean arterial pressure (117±2.2 vs 100±2.8 mmHg) and an increase in heart rate (570±32 vs 655±15 bpm) in ELA-2 KO mice. Despite a tendency towards reduction in the overall heart rate variability (standard deviation of successive values: 7.6±1.1 vs 4.7±0.6 ms, P=0.08), no changes were found in the root of the mean sum of squares or in the power of the high-frequency band. ACE inhibition did not change the spontaneous baroreflex indices (gain and baroreflex effectiveness index) in ELA-2 KO mice. Altogether, this data suggested that ACE played a role in the maintenance of hemodynamic function in ELA-2 KO mice. Associação Brasileira de Divulgação Científica 2022-03-21 /pmc/articles/PMC8939506/ /pubmed/35319673 http://dx.doi.org/10.1590/1414-431X2022e11774 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Prates-Costa, T.C. de Oliveira, M. Fazan, R. Salgado, H.C. Becari, C. Impact of angiotensin-converting enzyme inhibition on hemodynamic and autonomic profile of elastase-2 knockout mice |
title | Impact of angiotensin-converting enzyme inhibition on hemodynamic and
autonomic profile of elastase-2 knockout mice |
title_full | Impact of angiotensin-converting enzyme inhibition on hemodynamic and
autonomic profile of elastase-2 knockout mice |
title_fullStr | Impact of angiotensin-converting enzyme inhibition on hemodynamic and
autonomic profile of elastase-2 knockout mice |
title_full_unstemmed | Impact of angiotensin-converting enzyme inhibition on hemodynamic and
autonomic profile of elastase-2 knockout mice |
title_short | Impact of angiotensin-converting enzyme inhibition on hemodynamic and
autonomic profile of elastase-2 knockout mice |
title_sort | impact of angiotensin-converting enzyme inhibition on hemodynamic and
autonomic profile of elastase-2 knockout mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8939506/ https://www.ncbi.nlm.nih.gov/pubmed/35319673 http://dx.doi.org/10.1590/1414-431X2022e11774 |
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