Cargando…
Unraveling structural and conformational dynamics of DGAT1 missense nsSNPs in dairy cattle
Cattle are domestic animals that have been nourishing humans for thousands of years. Milk from cattle represents a key source of high-quality protein, fat, and other nutrients. The nutritional value of milk and dairy products is closely associated with the fat content, providing up to 30% of the tot...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8940929/ https://www.ncbi.nlm.nih.gov/pubmed/35318385 http://dx.doi.org/10.1038/s41598-022-08833-6 |
_version_ | 1784673002816798720 |
---|---|
author | Pathak, Rajesh Kumar Lim, Byeonghwi Park, Yejee Kim, Jun-Mo |
author_facet | Pathak, Rajesh Kumar Lim, Byeonghwi Park, Yejee Kim, Jun-Mo |
author_sort | Pathak, Rajesh Kumar |
collection | PubMed |
description | Cattle are domestic animals that have been nourishing humans for thousands of years. Milk from cattle represents a key source of high-quality protein, fat, and other nutrients. The nutritional value of milk and dairy products is closely associated with the fat content, providing up to 30% of the total fat consumed in the human diet. The fat content in cattle milk represents a major concern for the scientific community due to its association with human health. The relationship between milk fat content and diacylglycerol o-acyltransferase 1 gene (DGAT1) is well described in literature. Several studies demonstrated the difference in fat contents and other milk production traits in a wide range of cattle breeds, to be associated with missense non-synonymous single nucleotide polymorphisms (nsSNPs) of the DGAT1 gene. As a result, an nsSNPs analysis is crucial for unraveling the DGAT1 structural and conformational dynamics linked to milk fat content. DGAT1-nsSNPs are yet to be studied in terms of their structural and functional impact. Therefore, state-of-the-art computational and structural genomic methods were used to analyze five selected variants (W128R, W214R, C215G, P245R, and W459G), along with the wild type DGAT1. Significant structural and conformational changes in the variants were observed. We illustrate how single amino acid substitutions affect DGAT1 function, how this contributes to our understanding of the molecular basis of variations in DGAT1, and ultimately its impact in improving fat quality in milk. |
format | Online Article Text |
id | pubmed-8940929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89409292022-03-28 Unraveling structural and conformational dynamics of DGAT1 missense nsSNPs in dairy cattle Pathak, Rajesh Kumar Lim, Byeonghwi Park, Yejee Kim, Jun-Mo Sci Rep Article Cattle are domestic animals that have been nourishing humans for thousands of years. Milk from cattle represents a key source of high-quality protein, fat, and other nutrients. The nutritional value of milk and dairy products is closely associated with the fat content, providing up to 30% of the total fat consumed in the human diet. The fat content in cattle milk represents a major concern for the scientific community due to its association with human health. The relationship between milk fat content and diacylglycerol o-acyltransferase 1 gene (DGAT1) is well described in literature. Several studies demonstrated the difference in fat contents and other milk production traits in a wide range of cattle breeds, to be associated with missense non-synonymous single nucleotide polymorphisms (nsSNPs) of the DGAT1 gene. As a result, an nsSNPs analysis is crucial for unraveling the DGAT1 structural and conformational dynamics linked to milk fat content. DGAT1-nsSNPs are yet to be studied in terms of their structural and functional impact. Therefore, state-of-the-art computational and structural genomic methods were used to analyze five selected variants (W128R, W214R, C215G, P245R, and W459G), along with the wild type DGAT1. Significant structural and conformational changes in the variants were observed. We illustrate how single amino acid substitutions affect DGAT1 function, how this contributes to our understanding of the molecular basis of variations in DGAT1, and ultimately its impact in improving fat quality in milk. Nature Publishing Group UK 2022-03-22 /pmc/articles/PMC8940929/ /pubmed/35318385 http://dx.doi.org/10.1038/s41598-022-08833-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pathak, Rajesh Kumar Lim, Byeonghwi Park, Yejee Kim, Jun-Mo Unraveling structural and conformational dynamics of DGAT1 missense nsSNPs in dairy cattle |
title | Unraveling structural and conformational dynamics of DGAT1 missense nsSNPs in dairy cattle |
title_full | Unraveling structural and conformational dynamics of DGAT1 missense nsSNPs in dairy cattle |
title_fullStr | Unraveling structural and conformational dynamics of DGAT1 missense nsSNPs in dairy cattle |
title_full_unstemmed | Unraveling structural and conformational dynamics of DGAT1 missense nsSNPs in dairy cattle |
title_short | Unraveling structural and conformational dynamics of DGAT1 missense nsSNPs in dairy cattle |
title_sort | unraveling structural and conformational dynamics of dgat1 missense nssnps in dairy cattle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8940929/ https://www.ncbi.nlm.nih.gov/pubmed/35318385 http://dx.doi.org/10.1038/s41598-022-08833-6 |
work_keys_str_mv | AT pathakrajeshkumar unravelingstructuralandconformationaldynamicsofdgat1missensenssnpsindairycattle AT limbyeonghwi unravelingstructuralandconformationaldynamicsofdgat1missensenssnpsindairycattle AT parkyejee unravelingstructuralandconformationaldynamicsofdgat1missensenssnpsindairycattle AT kimjunmo unravelingstructuralandconformationaldynamicsofdgat1missensenssnpsindairycattle |