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Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-B(1) contaminated (Zea mays L.) seeds: a preliminary study
Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins. Besides being carcinogenic, they have other adverse health effects on humans and animals. In this preliminary study, we used two non-invasive optical techniques, optical fiber fluorescence spectroscopy...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8940939/ https://www.ncbi.nlm.nih.gov/pubmed/35318372 http://dx.doi.org/10.1038/s41598-022-08352-4 |
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author | Bartolić, Dragana Mutavdžić, Dragosav Carstensen, Jens Michael Stanković, Slavica Nikolić, Milica Krstović, Saša Radotić, Ksenija |
author_facet | Bartolić, Dragana Mutavdžić, Dragosav Carstensen, Jens Michael Stanković, Slavica Nikolić, Milica Krstović, Saša Radotić, Ksenija |
author_sort | Bartolić, Dragana |
collection | PubMed |
description | Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins. Besides being carcinogenic, they have other adverse health effects on humans and animals. In this preliminary study, we used two non-invasive optical techniques, optical fiber fluorescence spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally contaminated with aflatoxin B(1) (AFB(1)) from the uncontaminated seeds. The AFB(1)-contaminated seeds exhibited a red shift of the emission maximum position compared to the control samples. Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100% was obtained to discriminate uncontaminated and AFB(1)-contaminated seeds. The MSI analysis combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns of the analysed seeds. The AFB(1)-contaminated seeds showed a 7.9 to 9.6-fold increase in the seed reflectance in the VIS region, and 10.4 and 12.2-fold increase in the NIR spectral region, compared with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from uncontaminated seeds with high accuracy. The results may have an impact on development of spectroscopic non-invasive methods for detection of AFs presence in seeds, providing valuable information for the assessment of seed adulteration in the field of food forensics and food safety. |
format | Online Article Text |
id | pubmed-8940939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89409392022-03-28 Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-B(1) contaminated (Zea mays L.) seeds: a preliminary study Bartolić, Dragana Mutavdžić, Dragosav Carstensen, Jens Michael Stanković, Slavica Nikolić, Milica Krstović, Saša Radotić, Ksenija Sci Rep Article Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins. Besides being carcinogenic, they have other adverse health effects on humans and animals. In this preliminary study, we used two non-invasive optical techniques, optical fiber fluorescence spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally contaminated with aflatoxin B(1) (AFB(1)) from the uncontaminated seeds. The AFB(1)-contaminated seeds exhibited a red shift of the emission maximum position compared to the control samples. Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100% was obtained to discriminate uncontaminated and AFB(1)-contaminated seeds. The MSI analysis combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns of the analysed seeds. The AFB(1)-contaminated seeds showed a 7.9 to 9.6-fold increase in the seed reflectance in the VIS region, and 10.4 and 12.2-fold increase in the NIR spectral region, compared with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from uncontaminated seeds with high accuracy. The results may have an impact on development of spectroscopic non-invasive methods for detection of AFs presence in seeds, providing valuable information for the assessment of seed adulteration in the field of food forensics and food safety. Nature Publishing Group UK 2022-03-22 /pmc/articles/PMC8940939/ /pubmed/35318372 http://dx.doi.org/10.1038/s41598-022-08352-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Bartolić, Dragana Mutavdžić, Dragosav Carstensen, Jens Michael Stanković, Slavica Nikolić, Milica Krstović, Saša Radotić, Ksenija Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-B(1) contaminated (Zea mays L.) seeds: a preliminary study |
title | Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-B(1) contaminated (Zea mays L.) seeds: a preliminary study |
title_full | Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-B(1) contaminated (Zea mays L.) seeds: a preliminary study |
title_fullStr | Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-B(1) contaminated (Zea mays L.) seeds: a preliminary study |
title_full_unstemmed | Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-B(1) contaminated (Zea mays L.) seeds: a preliminary study |
title_short | Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-B(1) contaminated (Zea mays L.) seeds: a preliminary study |
title_sort | fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin-b(1) contaminated (zea mays l.) seeds: a preliminary study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8940939/ https://www.ncbi.nlm.nih.gov/pubmed/35318372 http://dx.doi.org/10.1038/s41598-022-08352-4 |
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