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Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs
Methionine (Met) as an essential amino acid has key importance in a variety of metabolic pathways. This study investigated the influence of three dietary Met supplements (0.21% L-Met, 0.21% DL-Met and 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA)) on the metabolome and inflammatory statu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941097/ https://www.ncbi.nlm.nih.gov/pubmed/35318410 http://dx.doi.org/10.1038/s41598-022-08933-3 |
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author | Schermuly, Isabel I. Romanet, Stella Klünemann, Martina Mastrototaro, Lucia Pieper, Robert Zentek, Jürgen Whelan, Rose A. Aschenbach, Jörg R. |
author_facet | Schermuly, Isabel I. Romanet, Stella Klünemann, Martina Mastrototaro, Lucia Pieper, Robert Zentek, Jürgen Whelan, Rose A. Aschenbach, Jörg R. |
author_sort | Schermuly, Isabel I. |
collection | PubMed |
description | Methionine (Met) as an essential amino acid has key importance in a variety of metabolic pathways. This study investigated the influence of three dietary Met supplements (0.21% L-Met, 0.21% DL-Met and 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA)) on the metabolome and inflammatory status in the small intestine of pigs. Epithelia from duodenum, proximal jejunum, middle jejunum and ileum were subjected to metabolomics analysis and qRT-PCR of caspase 1, NLR family pyrin domain containing 3 (NLRP3), interleukins IL1β, IL8, IL18, and transforming growth factor TGFβ. Principal component analysis of the intraepithelial metabolome revealed strong clustering of samples by intestinal segment but not by dietary treatment. However, pathway enrichment analysis revealed that after L-Met supplementation polyunsaturated fatty acids (PUFA) and tocopherol metabolites were lower across small intestinal segments, whereas monohydroxy fatty acids were increased in distal small intestine. Pigs supplemented with DL-HMTBA showed a pronounced shift of secondary bile acids (BA) and sphingosine metabolites from middle jejunum to ileum. In the amino acid super pathway, only histidine metabolism tended to be altered in DL-Met-supplemented pigs. Diet did not affect the expression of inflammation-related genes. These findings suggest that dietary supplementation of young pigs with different Met sources selectively alters lipid metabolism without consequences for inflammatory status. |
format | Online Article Text |
id | pubmed-8941097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89410972022-03-28 Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs Schermuly, Isabel I. Romanet, Stella Klünemann, Martina Mastrototaro, Lucia Pieper, Robert Zentek, Jürgen Whelan, Rose A. Aschenbach, Jörg R. Sci Rep Article Methionine (Met) as an essential amino acid has key importance in a variety of metabolic pathways. This study investigated the influence of three dietary Met supplements (0.21% L-Met, 0.21% DL-Met and 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA)) on the metabolome and inflammatory status in the small intestine of pigs. Epithelia from duodenum, proximal jejunum, middle jejunum and ileum were subjected to metabolomics analysis and qRT-PCR of caspase 1, NLR family pyrin domain containing 3 (NLRP3), interleukins IL1β, IL8, IL18, and transforming growth factor TGFβ. Principal component analysis of the intraepithelial metabolome revealed strong clustering of samples by intestinal segment but not by dietary treatment. However, pathway enrichment analysis revealed that after L-Met supplementation polyunsaturated fatty acids (PUFA) and tocopherol metabolites were lower across small intestinal segments, whereas monohydroxy fatty acids were increased in distal small intestine. Pigs supplemented with DL-HMTBA showed a pronounced shift of secondary bile acids (BA) and sphingosine metabolites from middle jejunum to ileum. In the amino acid super pathway, only histidine metabolism tended to be altered in DL-Met-supplemented pigs. Diet did not affect the expression of inflammation-related genes. These findings suggest that dietary supplementation of young pigs with different Met sources selectively alters lipid metabolism without consequences for inflammatory status. Nature Publishing Group UK 2022-03-22 /pmc/articles/PMC8941097/ /pubmed/35318410 http://dx.doi.org/10.1038/s41598-022-08933-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Schermuly, Isabel I. Romanet, Stella Klünemann, Martina Mastrototaro, Lucia Pieper, Robert Zentek, Jürgen Whelan, Rose A. Aschenbach, Jörg R. Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs |
title | Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs |
title_full | Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs |
title_fullStr | Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs |
title_full_unstemmed | Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs |
title_short | Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs |
title_sort | dietary methionine source alters the lipidome in the small intestinal epithelium of pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941097/ https://www.ncbi.nlm.nih.gov/pubmed/35318410 http://dx.doi.org/10.1038/s41598-022-08933-3 |
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