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Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

BACKGROUND: Clematis species are attractive ornamental plants with a variety of flower colors and patterns. Heat stress is one of the main factors restricting the growth, development, and ornamental value of Clematis. Clematis lanuginosa and Clematis crassifolia are large-flowered and evergreen Clem...

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Autores principales: Qian, Renjuan, Hu, Qingdi, Ma, Xiaohua, Zhang, Xule, Ye, Youju, Liu, Hongjian, Gao, Handong, Zheng, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941805/
https://www.ncbi.nlm.nih.gov/pubmed/35321648
http://dx.doi.org/10.1186/s12870-022-03497-w
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author Qian, Renjuan
Hu, Qingdi
Ma, Xiaohua
Zhang, Xule
Ye, Youju
Liu, Hongjian
Gao, Handong
Zheng, Jian
author_facet Qian, Renjuan
Hu, Qingdi
Ma, Xiaohua
Zhang, Xule
Ye, Youju
Liu, Hongjian
Gao, Handong
Zheng, Jian
author_sort Qian, Renjuan
collection PubMed
description BACKGROUND: Clematis species are attractive ornamental plants with a variety of flower colors and patterns. Heat stress is one of the main factors restricting the growth, development, and ornamental value of Clematis. Clematis lanuginosa and Clematis crassifolia are large-flowered and evergreen Clematis species, respectively, that show different tolerance to heat stress. We compared and analyzed the transcriptome of C. lanuginose and C. crassifolia under heat stress to determine the regulatory mechanism(s) of resistance. RESULTS: A total of 1720 and 6178 differentially expressed genes were identified from C. lanuginose and C. crassifolia, respectively. The photosynthesis and oxidation–reduction processes of C. crassifolia were more sensitive than C. lanuginose under heat stress. Glycine/serine/threonine metabolism, glyoxylic metabolism, and thiamine metabolism were important pathways in response to heat stress in C. lanuginose, and flavonoid biosynthesis, phenylalanine metabolism, and arginine/proline metabolism were the key pathways in C. crassifolia. Six sHSPs (c176964_g1, c200771_g1, c204924_g1, c199407_g2, c201522_g2, c192936_g1), POD1 (c200317_g1), POD3 (c210145_g2), DREB2 (c182557_g1), and HSFA2 (c206233_g2) may be key genes in the response to heat stress in C. lanuginose and C. crassifolia. CONCLUSIONS: We compared important metabolic pathways and differentially expressed genes in response to heat stress between C. lanuginose and C. crassifolia. The results increase our understanding of the response mechanism and candidate genes of Clematis under heat stress. These data may contribute to the development of new Clematis varieties with greater heat tolerance. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03497-w.
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spelling pubmed-89418052022-03-24 Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia Qian, Renjuan Hu, Qingdi Ma, Xiaohua Zhang, Xule Ye, Youju Liu, Hongjian Gao, Handong Zheng, Jian BMC Plant Biol Research BACKGROUND: Clematis species are attractive ornamental plants with a variety of flower colors and patterns. Heat stress is one of the main factors restricting the growth, development, and ornamental value of Clematis. Clematis lanuginosa and Clematis crassifolia are large-flowered and evergreen Clematis species, respectively, that show different tolerance to heat stress. We compared and analyzed the transcriptome of C. lanuginose and C. crassifolia under heat stress to determine the regulatory mechanism(s) of resistance. RESULTS: A total of 1720 and 6178 differentially expressed genes were identified from C. lanuginose and C. crassifolia, respectively. The photosynthesis and oxidation–reduction processes of C. crassifolia were more sensitive than C. lanuginose under heat stress. Glycine/serine/threonine metabolism, glyoxylic metabolism, and thiamine metabolism were important pathways in response to heat stress in C. lanuginose, and flavonoid biosynthesis, phenylalanine metabolism, and arginine/proline metabolism were the key pathways in C. crassifolia. Six sHSPs (c176964_g1, c200771_g1, c204924_g1, c199407_g2, c201522_g2, c192936_g1), POD1 (c200317_g1), POD3 (c210145_g2), DREB2 (c182557_g1), and HSFA2 (c206233_g2) may be key genes in the response to heat stress in C. lanuginose and C. crassifolia. CONCLUSIONS: We compared important metabolic pathways and differentially expressed genes in response to heat stress between C. lanuginose and C. crassifolia. The results increase our understanding of the response mechanism and candidate genes of Clematis under heat stress. These data may contribute to the development of new Clematis varieties with greater heat tolerance. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03497-w. BioMed Central 2022-03-23 /pmc/articles/PMC8941805/ /pubmed/35321648 http://dx.doi.org/10.1186/s12870-022-03497-w Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Qian, Renjuan
Hu, Qingdi
Ma, Xiaohua
Zhang, Xule
Ye, Youju
Liu, Hongjian
Gao, Handong
Zheng, Jian
Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia
title Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia
title_full Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia
title_fullStr Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia
title_full_unstemmed Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia
title_short Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia
title_sort comparative transcriptome analysis of heat stress responses of clematis lanuginosa and clematis crassifolia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941805/
https://www.ncbi.nlm.nih.gov/pubmed/35321648
http://dx.doi.org/10.1186/s12870-022-03497-w
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