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Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum

Cyanogenic glucosides (CGs) play a key role in host-plant defense to insect feeding; however, the metabolic tradeoffs between synthesis of CGs and plant growth are not well understood. In this study, genetic mutants coupled with nondestructive phenotyping techniques were used to study the impact of...

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Autores principales: Gruss, Shelby M., Ghaste, Manoj, Widhalm, Joshua R., Tuinstra, Mitchell R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8942933/
https://www.ncbi.nlm.nih.gov/pubmed/35001177
http://dx.doi.org/10.1007/s00122-021-04017-4
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author Gruss, Shelby M.
Ghaste, Manoj
Widhalm, Joshua R.
Tuinstra, Mitchell R.
author_facet Gruss, Shelby M.
Ghaste, Manoj
Widhalm, Joshua R.
Tuinstra, Mitchell R.
author_sort Gruss, Shelby M.
collection PubMed
description Cyanogenic glucosides (CGs) play a key role in host-plant defense to insect feeding; however, the metabolic tradeoffs between synthesis of CGs and plant growth are not well understood. In this study, genetic mutants coupled with nondestructive phenotyping techniques were used to study the impact of the CG dhurrin on fall armyworm [Spodoptera frugiperda (J.E. Smith)] (FAW) feeding and plant growth in sorghum [Sorghum bicolor (L.) Moench]. A genetic mutation in CYP79A1 gene that disrupts dhurrin biosynthesis was used to develop sets of near-isogenic lines (NILs) with contrasting dhurrin contents in the Tx623 bmr6 genetic background. The NILs were evaluated for differences in plant growth and FAW feeding damage in replicated greenhouse and field trials. Greenhouse studies showed that dhurrin-free Tx623 bmr6 cyp79a1 plants grew more quickly than wild-type plants but were more susceptible to insect feeding based on changes in green plant area (GPA), total leaf area, and total dry weight over time. The NILs exhibited similar patterns of growth in field trials with significant differences in leaf area and dry weight of dhurrin-free plants between the infested and non-infested treatments. Taken together, these studies reveal a significant metabolic tradeoff between CG biosynthesis and plant growth in sorghum seedlings. Disruption of dhurrin biosynthesis produces plants with higher growth rates than wild-type plants but these plants have greater susceptibility to FAW feeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-021-04017-4.
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spelling pubmed-89429332022-04-07 Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum Gruss, Shelby M. Ghaste, Manoj Widhalm, Joshua R. Tuinstra, Mitchell R. Theor Appl Genet Original Article Cyanogenic glucosides (CGs) play a key role in host-plant defense to insect feeding; however, the metabolic tradeoffs between synthesis of CGs and plant growth are not well understood. In this study, genetic mutants coupled with nondestructive phenotyping techniques were used to study the impact of the CG dhurrin on fall armyworm [Spodoptera frugiperda (J.E. Smith)] (FAW) feeding and plant growth in sorghum [Sorghum bicolor (L.) Moench]. A genetic mutation in CYP79A1 gene that disrupts dhurrin biosynthesis was used to develop sets of near-isogenic lines (NILs) with contrasting dhurrin contents in the Tx623 bmr6 genetic background. The NILs were evaluated for differences in plant growth and FAW feeding damage in replicated greenhouse and field trials. Greenhouse studies showed that dhurrin-free Tx623 bmr6 cyp79a1 plants grew more quickly than wild-type plants but were more susceptible to insect feeding based on changes in green plant area (GPA), total leaf area, and total dry weight over time. The NILs exhibited similar patterns of growth in field trials with significant differences in leaf area and dry weight of dhurrin-free plants between the infested and non-infested treatments. Taken together, these studies reveal a significant metabolic tradeoff between CG biosynthesis and plant growth in sorghum seedlings. Disruption of dhurrin biosynthesis produces plants with higher growth rates than wild-type plants but these plants have greater susceptibility to FAW feeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-021-04017-4. Springer Berlin Heidelberg 2022-01-09 2022 /pmc/articles/PMC8942933/ /pubmed/35001177 http://dx.doi.org/10.1007/s00122-021-04017-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Gruss, Shelby M.
Ghaste, Manoj
Widhalm, Joshua R.
Tuinstra, Mitchell R.
Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum
title Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum
title_full Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum
title_fullStr Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum
title_full_unstemmed Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum
title_short Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum
title_sort seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8942933/
https://www.ncbi.nlm.nih.gov/pubmed/35001177
http://dx.doi.org/10.1007/s00122-021-04017-4
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