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Single cell transcriptional diversity and intercellular crosstalk of human liver cancer
Liver cancer arises from the evolutionary selection of the dynamic tumor microenvironment (TME), in which the tumor cell generally becomes more heterogeneous; however, the mechanisms of TME-mediated transcriptional diversity of liver cancer remain unclear. Here, we assess transcriptional diversity i...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943132/ https://www.ncbi.nlm.nih.gov/pubmed/35322024 http://dx.doi.org/10.1038/s41419-022-04689-w |
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author | Meng, Yan Sang, Yan Liao, Jianping Zhao, Qiudong Qu, Shuping Li, Rong Jiang, Jinghua Wang, Meifeng Wang, Jiahong Wu, Dong Cheng, Chun Wei, Lixin |
author_facet | Meng, Yan Sang, Yan Liao, Jianping Zhao, Qiudong Qu, Shuping Li, Rong Jiang, Jinghua Wang, Meifeng Wang, Jiahong Wu, Dong Cheng, Chun Wei, Lixin |
author_sort | Meng, Yan |
collection | PubMed |
description | Liver cancer arises from the evolutionary selection of the dynamic tumor microenvironment (TME), in which the tumor cell generally becomes more heterogeneous; however, the mechanisms of TME-mediated transcriptional diversity of liver cancer remain unclear. Here, we assess transcriptional diversity in 15 liver cancer patients by single-cell transcriptome analysis and observe transcriptional diversity of tumor cells is associated with stemness in liver cancer patients. Tumor-associated fibroblast (TAF), as a potential driving force behind the heterogeneity in tumor cells within and between tumors, was predicted to interact with high heterogeneous tumor cells via COL1A1-ITGA2. Moreover, COL1A1-mediated YAP-signaling activation might be the mechanistic link between TAF and tumor cells with increased transcriptional diversity. Strikingly, the levels of COL1A1, ITGA2, and YAP are associated with morphological heterogeneity and poor overall survival of liver cancer patients. Beyond providing a potential mechanistic link between the TME and heterogeneous tumor cells, this study establishes that collagen-stimulated YAP activation is associates with transcriptional diversity in tumor cells by upregulating stemness, providing a theoretical basis for individualized treatment targets. |
format | Online Article Text |
id | pubmed-8943132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89431322022-04-08 Single cell transcriptional diversity and intercellular crosstalk of human liver cancer Meng, Yan Sang, Yan Liao, Jianping Zhao, Qiudong Qu, Shuping Li, Rong Jiang, Jinghua Wang, Meifeng Wang, Jiahong Wu, Dong Cheng, Chun Wei, Lixin Cell Death Dis Article Liver cancer arises from the evolutionary selection of the dynamic tumor microenvironment (TME), in which the tumor cell generally becomes more heterogeneous; however, the mechanisms of TME-mediated transcriptional diversity of liver cancer remain unclear. Here, we assess transcriptional diversity in 15 liver cancer patients by single-cell transcriptome analysis and observe transcriptional diversity of tumor cells is associated with stemness in liver cancer patients. Tumor-associated fibroblast (TAF), as a potential driving force behind the heterogeneity in tumor cells within and between tumors, was predicted to interact with high heterogeneous tumor cells via COL1A1-ITGA2. Moreover, COL1A1-mediated YAP-signaling activation might be the mechanistic link between TAF and tumor cells with increased transcriptional diversity. Strikingly, the levels of COL1A1, ITGA2, and YAP are associated with morphological heterogeneity and poor overall survival of liver cancer patients. Beyond providing a potential mechanistic link between the TME and heterogeneous tumor cells, this study establishes that collagen-stimulated YAP activation is associates with transcriptional diversity in tumor cells by upregulating stemness, providing a theoretical basis for individualized treatment targets. Nature Publishing Group UK 2022-03-24 /pmc/articles/PMC8943132/ /pubmed/35322024 http://dx.doi.org/10.1038/s41419-022-04689-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Meng, Yan Sang, Yan Liao, Jianping Zhao, Qiudong Qu, Shuping Li, Rong Jiang, Jinghua Wang, Meifeng Wang, Jiahong Wu, Dong Cheng, Chun Wei, Lixin Single cell transcriptional diversity and intercellular crosstalk of human liver cancer |
title | Single cell transcriptional diversity and intercellular crosstalk of human liver cancer |
title_full | Single cell transcriptional diversity and intercellular crosstalk of human liver cancer |
title_fullStr | Single cell transcriptional diversity and intercellular crosstalk of human liver cancer |
title_full_unstemmed | Single cell transcriptional diversity and intercellular crosstalk of human liver cancer |
title_short | Single cell transcriptional diversity and intercellular crosstalk of human liver cancer |
title_sort | single cell transcriptional diversity and intercellular crosstalk of human liver cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943132/ https://www.ncbi.nlm.nih.gov/pubmed/35322024 http://dx.doi.org/10.1038/s41419-022-04689-w |
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