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Prevalence and antimicrobial resistance of Campylobacter jejuni and C. coli identified in a slaughterhouse in Argentina
The aim of this study was to evaluate the percentage of Campylobacter (C. jejuni and C. coli) from samples collected at the slaughterhouse to describe the prevalence of resistance to selected antimicrobials, and to characterize the genetic determinants. In total, from 333 samples analyzed, 31% were...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943338/ https://www.ncbi.nlm.nih.gov/pubmed/35340997 http://dx.doi.org/10.1016/j.crfs.2022.03.005 |
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author | Schreyer, Mariana E. Olivero, Carolina R. Rossler, Eugenia Soto, Lorena P. Frizzo, Laureano S. Zimmermann, Jorge A. Signorini, Marcelo L. Virginia, Zbrun M. |
author_facet | Schreyer, Mariana E. Olivero, Carolina R. Rossler, Eugenia Soto, Lorena P. Frizzo, Laureano S. Zimmermann, Jorge A. Signorini, Marcelo L. Virginia, Zbrun M. |
author_sort | Schreyer, Mariana E. |
collection | PubMed |
description | The aim of this study was to evaluate the percentage of Campylobacter (C. jejuni and C. coli) from samples collected at the slaughterhouse to describe the prevalence of resistance to selected antimicrobials, and to characterize the genetic determinants. In total, from 333 samples analyzed, 31% were positive for Campylobacter. More positive samples were detected before the chiller (46%) than after the chiller (16%). C. coli (59%) was more prevalent than C. jejuni (41%). Antimicrobial resistance differences between C. jejuni and C. coli were found (p < 0.001). Multidrug resistance was found in 72% of C. coli isolates and 69% of C. jejuni isolates (p < 0.001). Most C. jejuni isolates (57%) had the three genes of the cmeABC efflux pump. The tet(O) gene and resistance-associated point mutations within both the gyrA and 23S rRNA genes were detected in 100% of C. coli isolates. On the other hand, C. jejuni only had more prevalence of the bla(OXA-61) gene than C. coli (p < 0.001), and most of the C. jejuni isolates (70–80%) had the tet(O) and gyrA point mutation. These results could contribute to knowledge about the status of thermotolerant Campylobacter resistant to antimicrobials isolated from food animals in Argentina and to develop an antimicrobial resistance surveillance system. |
format | Online Article Text |
id | pubmed-8943338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89433382022-03-25 Prevalence and antimicrobial resistance of Campylobacter jejuni and C. coli identified in a slaughterhouse in Argentina Schreyer, Mariana E. Olivero, Carolina R. Rossler, Eugenia Soto, Lorena P. Frizzo, Laureano S. Zimmermann, Jorge A. Signorini, Marcelo L. Virginia, Zbrun M. Curr Res Food Sci Research Article The aim of this study was to evaluate the percentage of Campylobacter (C. jejuni and C. coli) from samples collected at the slaughterhouse to describe the prevalence of resistance to selected antimicrobials, and to characterize the genetic determinants. In total, from 333 samples analyzed, 31% were positive for Campylobacter. More positive samples were detected before the chiller (46%) than after the chiller (16%). C. coli (59%) was more prevalent than C. jejuni (41%). Antimicrobial resistance differences between C. jejuni and C. coli were found (p < 0.001). Multidrug resistance was found in 72% of C. coli isolates and 69% of C. jejuni isolates (p < 0.001). Most C. jejuni isolates (57%) had the three genes of the cmeABC efflux pump. The tet(O) gene and resistance-associated point mutations within both the gyrA and 23S rRNA genes were detected in 100% of C. coli isolates. On the other hand, C. jejuni only had more prevalence of the bla(OXA-61) gene than C. coli (p < 0.001), and most of the C. jejuni isolates (70–80%) had the tet(O) and gyrA point mutation. These results could contribute to knowledge about the status of thermotolerant Campylobacter resistant to antimicrobials isolated from food animals in Argentina and to develop an antimicrobial resistance surveillance system. Elsevier 2022-03-17 /pmc/articles/PMC8943338/ /pubmed/35340997 http://dx.doi.org/10.1016/j.crfs.2022.03.005 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Schreyer, Mariana E. Olivero, Carolina R. Rossler, Eugenia Soto, Lorena P. Frizzo, Laureano S. Zimmermann, Jorge A. Signorini, Marcelo L. Virginia, Zbrun M. Prevalence and antimicrobial resistance of Campylobacter jejuni and C. coli identified in a slaughterhouse in Argentina |
title | Prevalence and antimicrobial resistance of Campylobacter jejuni and C. coli identified in a slaughterhouse in Argentina |
title_full | Prevalence and antimicrobial resistance of Campylobacter jejuni and C. coli identified in a slaughterhouse in Argentina |
title_fullStr | Prevalence and antimicrobial resistance of Campylobacter jejuni and C. coli identified in a slaughterhouse in Argentina |
title_full_unstemmed | Prevalence and antimicrobial resistance of Campylobacter jejuni and C. coli identified in a slaughterhouse in Argentina |
title_short | Prevalence and antimicrobial resistance of Campylobacter jejuni and C. coli identified in a slaughterhouse in Argentina |
title_sort | prevalence and antimicrobial resistance of campylobacter jejuni and c. coli identified in a slaughterhouse in argentina |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943338/ https://www.ncbi.nlm.nih.gov/pubmed/35340997 http://dx.doi.org/10.1016/j.crfs.2022.03.005 |
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