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Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer

BACKGROUND: Leukocyte immunoglobulin-like receptor subfamily B (LILRB), including 5 subtypes, is a group of inhibitory receptors in the immune system. The LILRB family is known to be involved in the tumor progression of various cancer types, especially liver cancer. However, the expression patterns...

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Autores principales: Fan, Jing, Wang, Lili, Chen, Miao, Zhang, Jiakang, Li, Jiayan, Song, Fangnan, Gu, Aidong, Yin, Dandan, Yi, Yongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943947/
https://www.ncbi.nlm.nih.gov/pubmed/35321724
http://dx.doi.org/10.1186/s12957-022-02562-w
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author Fan, Jing
Wang, Lili
Chen, Miao
Zhang, Jiakang
Li, Jiayan
Song, Fangnan
Gu, Aidong
Yin, Dandan
Yi, Yongxiang
author_facet Fan, Jing
Wang, Lili
Chen, Miao
Zhang, Jiakang
Li, Jiayan
Song, Fangnan
Gu, Aidong
Yin, Dandan
Yi, Yongxiang
author_sort Fan, Jing
collection PubMed
description BACKGROUND: Leukocyte immunoglobulin-like receptor subfamily B (LILRB), including 5 subtypes, is a group of inhibitory receptors in the immune system. The LILRB family is known to be involved in the tumor progression of various cancer types, especially liver cancer. However, the expression patterns and prognostic values of LILRB family members in liver cancer tissues remain unclear. METHODS: We used the Oncomine database, GEPIA database, Kaplan–Meier Plotter, Timer, and TISIDB to assess the expression and prognostic value of the LILRB family in liver cancer patients. We also verified the expression of the LILRB family in tumor tissues and tumor-free liver tissues at the protein level by using immunohistochemistry. The STRING website was used to explore the interaction between the LILRB family and their related genes. The DAVID database was used to perform the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Flow cytometry was used to assess the infiltrated NK cells in liver cancer tissues. RESULTS: Our study revealed that the mRNA expression of LILRB1, LILRB2, LILRB3, and LILRB5 was downregulated, while compared with normal tissues, the mRNA expression of LILRB4 was upregulated in liver cancer tissues. Survival analysis revealed that LILRB2 and LILRB5 mRNA expression levels were significantly positively associated with overall survival (OS) and disease-free survival (DSS) and that the mRNA expression of all LILRB family members was significantly positively correlated with recurrence-free survival (RFS) and progression-free survival (PFS). Next, we further found that the mRNA expression of all LILRB family members was significantly associated with the infiltration of B cells, CD8(+) T cells, CD4(+) T cells, macrophages, neutrophils, and dendritic cells in liver cancer. Finally, GO and KEGG analyses found that the LILRB family and its related genes were involved in antigen processing and presentation and natural killer cell-mediated cytotoxicity pathways. CONCLUSIONS: Our study suggested that LILRB family expression was associated with the prognosis of liver cancer patients and infiltrated immune cells. The LILRB family might be involved in antigen processing and presentation and natural killer cell-mediated cytotoxicity pathways. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12957-022-02562-w.
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spelling pubmed-89439472022-03-25 Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer Fan, Jing Wang, Lili Chen, Miao Zhang, Jiakang Li, Jiayan Song, Fangnan Gu, Aidong Yin, Dandan Yi, Yongxiang World J Surg Oncol Research BACKGROUND: Leukocyte immunoglobulin-like receptor subfamily B (LILRB), including 5 subtypes, is a group of inhibitory receptors in the immune system. The LILRB family is known to be involved in the tumor progression of various cancer types, especially liver cancer. However, the expression patterns and prognostic values of LILRB family members in liver cancer tissues remain unclear. METHODS: We used the Oncomine database, GEPIA database, Kaplan–Meier Plotter, Timer, and TISIDB to assess the expression and prognostic value of the LILRB family in liver cancer patients. We also verified the expression of the LILRB family in tumor tissues and tumor-free liver tissues at the protein level by using immunohistochemistry. The STRING website was used to explore the interaction between the LILRB family and their related genes. The DAVID database was used to perform the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Flow cytometry was used to assess the infiltrated NK cells in liver cancer tissues. RESULTS: Our study revealed that the mRNA expression of LILRB1, LILRB2, LILRB3, and LILRB5 was downregulated, while compared with normal tissues, the mRNA expression of LILRB4 was upregulated in liver cancer tissues. Survival analysis revealed that LILRB2 and LILRB5 mRNA expression levels were significantly positively associated with overall survival (OS) and disease-free survival (DSS) and that the mRNA expression of all LILRB family members was significantly positively correlated with recurrence-free survival (RFS) and progression-free survival (PFS). Next, we further found that the mRNA expression of all LILRB family members was significantly associated with the infiltration of B cells, CD8(+) T cells, CD4(+) T cells, macrophages, neutrophils, and dendritic cells in liver cancer. Finally, GO and KEGG analyses found that the LILRB family and its related genes were involved in antigen processing and presentation and natural killer cell-mediated cytotoxicity pathways. CONCLUSIONS: Our study suggested that LILRB family expression was associated with the prognosis of liver cancer patients and infiltrated immune cells. The LILRB family might be involved in antigen processing and presentation and natural killer cell-mediated cytotoxicity pathways. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12957-022-02562-w. BioMed Central 2022-03-24 /pmc/articles/PMC8943947/ /pubmed/35321724 http://dx.doi.org/10.1186/s12957-022-02562-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Fan, Jing
Wang, Lili
Chen, Miao
Zhang, Jiakang
Li, Jiayan
Song, Fangnan
Gu, Aidong
Yin, Dandan
Yi, Yongxiang
Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer
title Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer
title_full Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer
title_fullStr Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer
title_full_unstemmed Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer
title_short Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer
title_sort analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily b in human liver cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943947/
https://www.ncbi.nlm.nih.gov/pubmed/35321724
http://dx.doi.org/10.1186/s12957-022-02562-w
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