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Pattern of chromosomal aberrations persisting over 30 years in a Chernobyl Nuclear Power Plant accident survivor: study using mFISH

The long-term in vivo cytogenetic effects of high-dose radiation exposure can be traced in accidentally irradiated persons, and particularly useful for developing strategies of monitoring and therapy of such patients, as well as for elucidating the fundamental aspects of hematopoiesis and radiobiolo...

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Autores principales: Nikitina, Victoriya, Nugis, Vladimir, Astrelina, Tatiyana, Zheglo, Diana, Kobzeva, Irina, Kozlova, Mariya, Galstyan, Irina, Lomonosova, Elena, Zhanataev, Aliy, Karaseva, Tatiyana, Samoylov, Alexander S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944318/
https://www.ncbi.nlm.nih.gov/pubmed/35146520
http://dx.doi.org/10.1093/jrr/rrab131
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author Nikitina, Victoriya
Nugis, Vladimir
Astrelina, Tatiyana
Zheglo, Diana
Kobzeva, Irina
Kozlova, Mariya
Galstyan, Irina
Lomonosova, Elena
Zhanataev, Aliy
Karaseva, Tatiyana
Samoylov, Alexander S
author_facet Nikitina, Victoriya
Nugis, Vladimir
Astrelina, Tatiyana
Zheglo, Diana
Kobzeva, Irina
Kozlova, Mariya
Galstyan, Irina
Lomonosova, Elena
Zhanataev, Aliy
Karaseva, Tatiyana
Samoylov, Alexander S
author_sort Nikitina, Victoriya
collection PubMed
description The long-term in vivo cytogenetic effects of high-dose radiation exposure can be traced in accidentally irradiated persons, and particularly useful for developing strategies of monitoring and therapy of such patients, as well as for elucidating the fundamental aspects of hematopoiesis and radiobiology. Using 24-color fluorescent in situ hybridization (mFISH), we analysed the frequency and the spectrum of chromosomal aberrations (CA) in peripheral blood lymphocytes of the Chernobyl Nuclear Power Plant (NPP) accident victim 30, 31, 32 and 33 years after acute accidental exposure to high-dose gamma radiation of the whole body. Totally, 993 metaphase cells were analyzed (or 219, 272, 258, 244 cells each year), of which 297 were aberrant. Our study demonstrated a constant aberrant cell frequency at 28% in 2016–2018 years, while in 2019, a significant increase up to 35% occurred due to contribution of significantly elevated frequency of simple aberrations in the absence of evident recent genotoxic factors. Four clonal aberrations were detected, three of which persisted for more than one year at a frequency up to 2.5% of analyzed cells. The distribution of 731 breakpoints per individual chromosomes was nearly proportional to their physical length, excepting Chromosomes 13 and 20, which were significantly breakpoint-deficient compared to the genome median rate. Monitoring of the long-term effects on chromosomal instability caused by radiation exposure is important for understanding and predicting the long-term effects of ionizing radiation.
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spelling pubmed-89443182022-03-28 Pattern of chromosomal aberrations persisting over 30 years in a Chernobyl Nuclear Power Plant accident survivor: study using mFISH Nikitina, Victoriya Nugis, Vladimir Astrelina, Tatiyana Zheglo, Diana Kobzeva, Irina Kozlova, Mariya Galstyan, Irina Lomonosova, Elena Zhanataev, Aliy Karaseva, Tatiyana Samoylov, Alexander S J Radiat Res Fundamental Radiation Science The long-term in vivo cytogenetic effects of high-dose radiation exposure can be traced in accidentally irradiated persons, and particularly useful for developing strategies of monitoring and therapy of such patients, as well as for elucidating the fundamental aspects of hematopoiesis and radiobiology. Using 24-color fluorescent in situ hybridization (mFISH), we analysed the frequency and the spectrum of chromosomal aberrations (CA) in peripheral blood lymphocytes of the Chernobyl Nuclear Power Plant (NPP) accident victim 30, 31, 32 and 33 years after acute accidental exposure to high-dose gamma radiation of the whole body. Totally, 993 metaphase cells were analyzed (or 219, 272, 258, 244 cells each year), of which 297 were aberrant. Our study demonstrated a constant aberrant cell frequency at 28% in 2016–2018 years, while in 2019, a significant increase up to 35% occurred due to contribution of significantly elevated frequency of simple aberrations in the absence of evident recent genotoxic factors. Four clonal aberrations were detected, three of which persisted for more than one year at a frequency up to 2.5% of analyzed cells. The distribution of 731 breakpoints per individual chromosomes was nearly proportional to their physical length, excepting Chromosomes 13 and 20, which were significantly breakpoint-deficient compared to the genome median rate. Monitoring of the long-term effects on chromosomal instability caused by radiation exposure is important for understanding and predicting the long-term effects of ionizing radiation. Oxford University Press 2022-02-11 /pmc/articles/PMC8944318/ /pubmed/35146520 http://dx.doi.org/10.1093/jrr/rrab131 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Fundamental Radiation Science
Nikitina, Victoriya
Nugis, Vladimir
Astrelina, Tatiyana
Zheglo, Diana
Kobzeva, Irina
Kozlova, Mariya
Galstyan, Irina
Lomonosova, Elena
Zhanataev, Aliy
Karaseva, Tatiyana
Samoylov, Alexander S
Pattern of chromosomal aberrations persisting over 30 years in a Chernobyl Nuclear Power Plant accident survivor: study using mFISH
title Pattern of chromosomal aberrations persisting over 30 years in a Chernobyl Nuclear Power Plant accident survivor: study using mFISH
title_full Pattern of chromosomal aberrations persisting over 30 years in a Chernobyl Nuclear Power Plant accident survivor: study using mFISH
title_fullStr Pattern of chromosomal aberrations persisting over 30 years in a Chernobyl Nuclear Power Plant accident survivor: study using mFISH
title_full_unstemmed Pattern of chromosomal aberrations persisting over 30 years in a Chernobyl Nuclear Power Plant accident survivor: study using mFISH
title_short Pattern of chromosomal aberrations persisting over 30 years in a Chernobyl Nuclear Power Plant accident survivor: study using mFISH
title_sort pattern of chromosomal aberrations persisting over 30 years in a chernobyl nuclear power plant accident survivor: study using mfish
topic Fundamental Radiation Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944318/
https://www.ncbi.nlm.nih.gov/pubmed/35146520
http://dx.doi.org/10.1093/jrr/rrab131
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