Clinical Efficacy and Safety of Malarone(®), Azithromycin and Artesunate Combination for Treatment of Babesia gibsoni in Naturally Infected Dogs

SIMPLE SUMMARY: Babesia gibsoni is an intracellular parasite of red blood cells that may cause anemia in dogs. Many drugs have been used in management of canine babesiosis such as monotherapy or combined treatment, but complete elimination of parasitemia was not proven, frequent relapses were observed and adverse effects occurred during treatment. This report examines the effectiveness and safety of Malarone(®), azithromycin (AZM) and artesunate (ART) combination for the treatment of babesiosis in dogs naturally infected with Babesia gibsoni. The treatment improved hematology and biochemical parameters to the reference range. No clinically apparent adverse effects were reported during treatment and monitoring and no relapses of parasitemia were detected during monitoring until day 720 after treatment. Results of the study indicate that the combined treatment leads to successful elimination of parasitemia in chronically infected dogs with B. gibsoni. ABSTRACT: Babesia gibsoni is a tick-borne protozoal blood parasite that may cause hemolytic anemia, thrombocytopenia, lethargy, and/or splenomegaly in dogs. Many drugs have been used in management of canine babesiosis such as monotherapy or combined treatment, including diminazene aceturate, imidocarb dipropionate, atovaquone, and antibiotics. This report examines the effectiveness and safety of Malarone(®), azithromycin (AZM) and artesunate (ART) combination for the treatment of babesiosis in dogs naturally infected with Babesia gibsoni. Twelve American Pit Bull Terriers were included in the experiment. Examined dogs underwent clinical and laboratory analysis including hematology and biochemistry profile and serum protein electrophoresis. After diagnosis, the dogs received combined therapy with Malarone(®) (13.5 mg/kg PO q24 h), azithromycin (10 mg/kg PO q24 h) and artesunate (12.5 mg/kg PO q24 h) for 10 days. The combined treatment improved hematology and biochemical parameters to the reference range gradually during the first 14 days already, resulting in the stable values until day 56 after treatment. No clinically apparent adverse effects were reported during treatment and monitoring. No relapses of parasitemia were detected in control days 180, 360, 540 and 720 in all dogs. Results of the study indicate that the combined treatment leads to successful elimination of parasitemia in chronically infected dogs with B. gibsoni.