Aerobic Commensal Conjunctival Microflora in Healthy Donkeys

SIMPLE SUMMARY: The mucosa of the conjunctival sac in mammals is colonized by a group of bacteria, and their interdependence is referred to as commensalism—the coexistence of two different species populations benefiting at least one of the partners. The presence of conjunctival bacterial commensal flora is very important for the capacity of the non-specific immune response. Some of the bacteria are described as conditionally pathogenic, meaning that in the case of breaking down protective barriers, they may act as pathogens. Knowledge of the commensal flora of healthy individuals is of great clinical importance in the diagnosis of pathological conditions. The aim of this study is to assess the presence and the composition of normal ocular microflora of healthy donkeys. To the best of the authors’ knowledge, this is the first report that provides knowledge about commensal microflora in the conjunctival sac of donkeys bred in Central Europe. ABSTRACT: From a clinical point of view, knowledge of the commensal microbial flora of the conjunctival sac in healthy individuals proves to be of great importance. The aim of this study was to assess the presence and the composition of normal ocular microflora of healthy donkeys. Fourteen clinically healthy donkeys (Equus asinus) participated in the study. After prior ophthalmological examination, which showed no abnormalities, a conjunctival swab was taken from each donkey from the right and left eye. Species-specific identification was based on a morphological assessment of bacterial colonies stained with the Gram technique, as well as on biochemical properties and the disk-diffusion method. Around 82% of samples were positive for bacteria cultivation; Pantoea agglomerans was the most prevalently detected species, followed by Moraxella lacunata. In conclusion, our study made it possible to determine the commensal flora of the conjunctival sac in donkeys. The obtained results also showed discrepancies in the composition of the conjunctival sac flora of donkeys and horses, despite the geographical proximity of performed studies. Knowledge of the commensal conjunctival flora of donkeys is of great clinical importance due to their greater exposure to corneal damage and infections than horses.