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Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries

Anthropogenic pressure is known to be a key driver of antimicrobial resistance (AMR) dissemination in the environment. Especially in lower income countries, with poor infrastructure, the level of AMR dissemination is high. Therefore, we assessed the levels and diversity of antibiotic-resistant bacte...

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Autores principales: Hobeika, Wadad, Gaschet, Margaux, Ploy, Marie-Cécile, Buelow, Elena, Sarkis, Dolla Karam, Dagot, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944630/
https://www.ncbi.nlm.nih.gov/pubmed/35326767
http://dx.doi.org/10.3390/antibiotics11030306
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author Hobeika, Wadad
Gaschet, Margaux
Ploy, Marie-Cécile
Buelow, Elena
Sarkis, Dolla Karam
Dagot, Christophe
author_facet Hobeika, Wadad
Gaschet, Margaux
Ploy, Marie-Cécile
Buelow, Elena
Sarkis, Dolla Karam
Dagot, Christophe
author_sort Hobeika, Wadad
collection PubMed
description Anthropogenic pressure is known to be a key driver of antimicrobial resistance (AMR) dissemination in the environment. Especially in lower income countries, with poor infrastructure, the level of AMR dissemination is high. Therefore, we assessed the levels and diversity of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in Lebanese rivers at estuaries’ sites (n = 72) of the Mediterranean Sea in spring 2017 and winter 2018. Methods: A combined approach using culture techniques and high throughput qPCR were applied to identify ARB and ARGs in rivers along the Lebanese coast. Results: Multidrug-resistant Gram-negative (Enterobacterales and Pseudomonas spp.) and Gram-positive bacterial pathogens were isolated. Levels of ARGs were highest in the winter campaign and areas with high anthropogenic activities and population growth with an influx of refugees. Conclusion: Qualitative analysis of ARB and the analysis of the Lebanese estuaries’ resistome revealed critical levels of contamination with pathogenic bacteria and provided significant information about the spread of ARGs in anthropogenically impacted estuaries.
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spelling pubmed-89446302022-03-25 Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries Hobeika, Wadad Gaschet, Margaux Ploy, Marie-Cécile Buelow, Elena Sarkis, Dolla Karam Dagot, Christophe Antibiotics (Basel) Article Anthropogenic pressure is known to be a key driver of antimicrobial resistance (AMR) dissemination in the environment. Especially in lower income countries, with poor infrastructure, the level of AMR dissemination is high. Therefore, we assessed the levels and diversity of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in Lebanese rivers at estuaries’ sites (n = 72) of the Mediterranean Sea in spring 2017 and winter 2018. Methods: A combined approach using culture techniques and high throughput qPCR were applied to identify ARB and ARGs in rivers along the Lebanese coast. Results: Multidrug-resistant Gram-negative (Enterobacterales and Pseudomonas spp.) and Gram-positive bacterial pathogens were isolated. Levels of ARGs were highest in the winter campaign and areas with high anthropogenic activities and population growth with an influx of refugees. Conclusion: Qualitative analysis of ARB and the analysis of the Lebanese estuaries’ resistome revealed critical levels of contamination with pathogenic bacteria and provided significant information about the spread of ARGs in anthropogenically impacted estuaries. MDPI 2022-02-24 /pmc/articles/PMC8944630/ /pubmed/35326767 http://dx.doi.org/10.3390/antibiotics11030306 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hobeika, Wadad
Gaschet, Margaux
Ploy, Marie-Cécile
Buelow, Elena
Sarkis, Dolla Karam
Dagot, Christophe
Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries
title Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries
title_full Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries
title_fullStr Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries
title_full_unstemmed Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries
title_short Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries
title_sort resistome diversity and dissemination of who priority antibiotic resistant pathogens in lebanese estuaries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944630/
https://www.ncbi.nlm.nih.gov/pubmed/35326767
http://dx.doi.org/10.3390/antibiotics11030306
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