Cargando…

Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples

The increasing number of nosocomial pathogens with resistances towards last resort antibiotics, like linezolid for gram positive bacteria, leads to a pressing need for screening and, consequently, suitable screening media. Some national guidelines on infection prevention (e.g., in Germany) have alre...

Descripción completa

Detalles Bibliográficos
Autores principales: Girlich, Delphine, Mihaila, Liliana, Cattoir, Vincent, Laurent, Frédéric, Begasse, Christine, David, Florence, Metro, Carole-Ann, Dortet, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944678/
https://www.ncbi.nlm.nih.gov/pubmed/35326776
http://dx.doi.org/10.3390/antibiotics11030313
_version_ 1784673776696295424
author Girlich, Delphine
Mihaila, Liliana
Cattoir, Vincent
Laurent, Frédéric
Begasse, Christine
David, Florence
Metro, Carole-Ann
Dortet, Laurent
author_facet Girlich, Delphine
Mihaila, Liliana
Cattoir, Vincent
Laurent, Frédéric
Begasse, Christine
David, Florence
Metro, Carole-Ann
Dortet, Laurent
author_sort Girlich, Delphine
collection PubMed
description The increasing number of nosocomial pathogens with resistances towards last resort antibiotics, like linezolid for gram positive bacteria, leads to a pressing need for screening and, consequently, suitable screening media. Some national guidelines on infection prevention (e.g., in Germany) have already recommended screening for linezolid-resistant bacteria, despite an accurate screening medium that was not available yet. In this study, we analyzed the performance and reliability of the first commercial chromogenic medium, CHOMagar™ LIN-R, for screening of linezolid-resistant gram-positive isolates. Thirty-four pure bacterial cultures, 18 positive blood cultures, and 358 nasal swab screening samples were tested. This medium efficiently detected linezolid-resistant S. epidermidis isolates from pure bacterial cultures and from positive blood cultures with a high sensitivity (100%) and specificity (100%). Among the 358 nasal swab screening samples prospectively tested, 10.9% were cultured with linezolid-resistant isolates (mostly S. epidermidis). Of note, slight growth was observed for 7.5% samples with linezolid-susceptible isolates of S. epidermidis (n = 1), S. aureus (n = 1), Enterococcus faecalis (n = 4), Lactobacillus spp. (n = 3), gram negatives (n = 18). Moreover, few Candida spp. also cultured on this medium (1.4%).
format Online
Article
Text
id pubmed-8944678
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89446782022-03-25 Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples Girlich, Delphine Mihaila, Liliana Cattoir, Vincent Laurent, Frédéric Begasse, Christine David, Florence Metro, Carole-Ann Dortet, Laurent Antibiotics (Basel) Article The increasing number of nosocomial pathogens with resistances towards last resort antibiotics, like linezolid for gram positive bacteria, leads to a pressing need for screening and, consequently, suitable screening media. Some national guidelines on infection prevention (e.g., in Germany) have already recommended screening for linezolid-resistant bacteria, despite an accurate screening medium that was not available yet. In this study, we analyzed the performance and reliability of the first commercial chromogenic medium, CHOMagar™ LIN-R, for screening of linezolid-resistant gram-positive isolates. Thirty-four pure bacterial cultures, 18 positive blood cultures, and 358 nasal swab screening samples were tested. This medium efficiently detected linezolid-resistant S. epidermidis isolates from pure bacterial cultures and from positive blood cultures with a high sensitivity (100%) and specificity (100%). Among the 358 nasal swab screening samples prospectively tested, 10.9% were cultured with linezolid-resistant isolates (mostly S. epidermidis). Of note, slight growth was observed for 7.5% samples with linezolid-susceptible isolates of S. epidermidis (n = 1), S. aureus (n = 1), Enterococcus faecalis (n = 4), Lactobacillus spp. (n = 3), gram negatives (n = 18). Moreover, few Candida spp. also cultured on this medium (1.4%). MDPI 2022-02-25 /pmc/articles/PMC8944678/ /pubmed/35326776 http://dx.doi.org/10.3390/antibiotics11030313 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Girlich, Delphine
Mihaila, Liliana
Cattoir, Vincent
Laurent, Frédéric
Begasse, Christine
David, Florence
Metro, Carole-Ann
Dortet, Laurent
Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples
title Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples
title_full Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples
title_fullStr Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples
title_full_unstemmed Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples
title_short Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples
title_sort evaluation of chromagar™ lin-r for the screening of linezolid resistant staphylococci from positive blood cultures and nasal swab screening samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944678/
https://www.ncbi.nlm.nih.gov/pubmed/35326776
http://dx.doi.org/10.3390/antibiotics11030313
work_keys_str_mv AT girlichdelphine evaluationofchromagarlinrforthescreeningoflinezolidresistantstaphylococcifrompositivebloodculturesandnasalswabscreeningsamples
AT mihailaliliana evaluationofchromagarlinrforthescreeningoflinezolidresistantstaphylococcifrompositivebloodculturesandnasalswabscreeningsamples
AT cattoirvincent evaluationofchromagarlinrforthescreeningoflinezolidresistantstaphylococcifrompositivebloodculturesandnasalswabscreeningsamples
AT laurentfrederic evaluationofchromagarlinrforthescreeningoflinezolidresistantstaphylococcifrompositivebloodculturesandnasalswabscreeningsamples
AT begassechristine evaluationofchromagarlinrforthescreeningoflinezolidresistantstaphylococcifrompositivebloodculturesandnasalswabscreeningsamples
AT davidflorence evaluationofchromagarlinrforthescreeningoflinezolidresistantstaphylococcifrompositivebloodculturesandnasalswabscreeningsamples
AT metrocaroleann evaluationofchromagarlinrforthescreeningoflinezolidresistantstaphylococcifrompositivebloodculturesandnasalswabscreeningsamples
AT dortetlaurent evaluationofchromagarlinrforthescreeningoflinezolidresistantstaphylococcifrompositivebloodculturesandnasalswabscreeningsamples