Cargando…
Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins
Kojic acid, β-arbutin, α-arbutin, and deoxyarbutin have been reported as tyrosinase inhibitors in many articles, but some contradictions exist in their differing results. In order to provide some explanations for these contradictions and to find the most suitable compound as a positive control for s...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944748/ https://www.ncbi.nlm.nih.gov/pubmed/35326152 http://dx.doi.org/10.3390/antiox11030502 |
_version_ | 1784673794796814336 |
---|---|
author | Wang, Wei Gao, Ying Wang, Weiwei Zhang, Jianyong Yin, Junfeng Le, Ting Xue, Jinjin Engelhardt, Ulrich H. Jiang, Heyuan |
author_facet | Wang, Wei Gao, Ying Wang, Weiwei Zhang, Jianyong Yin, Junfeng Le, Ting Xue, Jinjin Engelhardt, Ulrich H. Jiang, Heyuan |
author_sort | Wang, Wei |
collection | PubMed |
description | Kojic acid, β-arbutin, α-arbutin, and deoxyarbutin have been reported as tyrosinase inhibitors in many articles, but some contradictions exist in their differing results. In order to provide some explanations for these contradictions and to find the most suitable compound as a positive control for screening potential tyrosinase inhibitors, the activity and inhibition type of the aforementioned compounds on monophenolase and diphenolase of mushroom tyrosinase (MTYR) were studied. Their effects on B16F10 cells melanin content, tyrosinase (BTYR) activity, and cell viability were also exposed. Results indicated that α-arbutin competitively inhibited monophenolase activity, whereas they uncompetitively activated diphenolase activity of MTYR. β-arbutin noncompetitively and competitively inhibited monophenolase activity at high molarity (4000 µM) and moderate molarity (250–1000 µM) respectively, whereas it activated the diphenolase activity of MTYR. Deoxyarbutin competitively inhibited diphenolase activity, but could not inhibit monophenolase activity and only extended the lag time. Kojic acid competitively inhibited monophenolase activity and competitive–noncompetitive mixed-type inhibited diphenolase activity of MTYR. In a cellular experiment, deoxyarbutin effectively inhibited BTYR activity and reduced melanin content, but it also potently decreased cell viability. α-arbutin and β-arbutin dose-dependently inhibited BTYR activity, reduced melanin content, and increased cell viability. Kojic acid did not affect cell viability at 43.8–700 µM, but inhibited BTYR activity and reduced melanin content in a dose-dependent manner. Therefore, kojic acid was considered as the most suitable positive control among these four compounds, because it could inhibit both monophenolase and diphenolase activity of MTYR and reduce intercellular melanin content by inhibiting BTYR activity without cytotoxicity. Some explanations for the contradictions in the reported articles were provided. |
format | Online Article Text |
id | pubmed-8944748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89447482022-03-25 Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins Wang, Wei Gao, Ying Wang, Weiwei Zhang, Jianyong Yin, Junfeng Le, Ting Xue, Jinjin Engelhardt, Ulrich H. Jiang, Heyuan Antioxidants (Basel) Article Kojic acid, β-arbutin, α-arbutin, and deoxyarbutin have been reported as tyrosinase inhibitors in many articles, but some contradictions exist in their differing results. In order to provide some explanations for these contradictions and to find the most suitable compound as a positive control for screening potential tyrosinase inhibitors, the activity and inhibition type of the aforementioned compounds on monophenolase and diphenolase of mushroom tyrosinase (MTYR) were studied. Their effects on B16F10 cells melanin content, tyrosinase (BTYR) activity, and cell viability were also exposed. Results indicated that α-arbutin competitively inhibited monophenolase activity, whereas they uncompetitively activated diphenolase activity of MTYR. β-arbutin noncompetitively and competitively inhibited monophenolase activity at high molarity (4000 µM) and moderate molarity (250–1000 µM) respectively, whereas it activated the diphenolase activity of MTYR. Deoxyarbutin competitively inhibited diphenolase activity, but could not inhibit monophenolase activity and only extended the lag time. Kojic acid competitively inhibited monophenolase activity and competitive–noncompetitive mixed-type inhibited diphenolase activity of MTYR. In a cellular experiment, deoxyarbutin effectively inhibited BTYR activity and reduced melanin content, but it also potently decreased cell viability. α-arbutin and β-arbutin dose-dependently inhibited BTYR activity, reduced melanin content, and increased cell viability. Kojic acid did not affect cell viability at 43.8–700 µM, but inhibited BTYR activity and reduced melanin content in a dose-dependent manner. Therefore, kojic acid was considered as the most suitable positive control among these four compounds, because it could inhibit both monophenolase and diphenolase activity of MTYR and reduce intercellular melanin content by inhibiting BTYR activity without cytotoxicity. Some explanations for the contradictions in the reported articles were provided. MDPI 2022-03-04 /pmc/articles/PMC8944748/ /pubmed/35326152 http://dx.doi.org/10.3390/antiox11030502 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Wei Gao, Ying Wang, Weiwei Zhang, Jianyong Yin, Junfeng Le, Ting Xue, Jinjin Engelhardt, Ulrich H. Jiang, Heyuan Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins |
title | Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins |
title_full | Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins |
title_fullStr | Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins |
title_full_unstemmed | Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins |
title_short | Kojic Acid Showed Consistent Inhibitory Activity on Tyrosinase from Mushroom and in Cultured B16F10 Cells Compared with Arbutins |
title_sort | kojic acid showed consistent inhibitory activity on tyrosinase from mushroom and in cultured b16f10 cells compared with arbutins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944748/ https://www.ncbi.nlm.nih.gov/pubmed/35326152 http://dx.doi.org/10.3390/antiox11030502 |
work_keys_str_mv | AT wangwei kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins AT gaoying kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins AT wangweiwei kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins AT zhangjianyong kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins AT yinjunfeng kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins AT leting kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins AT xuejinjin kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins AT engelhardtulrichh kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins AT jiangheyuan kojicacidshowedconsistentinhibitoryactivityontyrosinasefrommushroomandinculturedb16f10cellscomparedwitharbutins |