Cargando…
The Symbiotic Effect of a New Nutraceutical with Yeast β-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1α, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model
The use of natural products and derivatives for the prevention and control of non-communicable chronic diseases, such as type-2 diabetes (T2D), obesity, and hepatic steatosis is a way to achieve homeostasis through different metabolic pathways. Thus, male C57BL/6 mice were divided into the following...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944780/ https://www.ncbi.nlm.nih.gov/pubmed/35326098 http://dx.doi.org/10.3390/antiox11030447 |
_version_ | 1784673802072883200 |
---|---|
author | Santamarina, Aline Boveto Moraes, Ruan Carlos Macêdo Nehmi Filho, Victor Murata, Gilson Masahiro de Freitas, Jéssica Alves de Miranda, Danielle Araujo Cerqueira, Anderson Romério Azevedo Costa, Soraia Katia Pereira Ferreira, Ana Flávia Fernandes Britto, Luiz Roberto de Camargo, Juliana Alves Rodrigues de Oliveira, Daniela de Jesus, Flavia Neto Otoch, José Pinhata Pessoa, Ana Flávia Marçal |
author_facet | Santamarina, Aline Boveto Moraes, Ruan Carlos Macêdo Nehmi Filho, Victor Murata, Gilson Masahiro de Freitas, Jéssica Alves de Miranda, Danielle Araujo Cerqueira, Anderson Romério Azevedo Costa, Soraia Katia Pereira Ferreira, Ana Flávia Fernandes Britto, Luiz Roberto de Camargo, Juliana Alves Rodrigues de Oliveira, Daniela de Jesus, Flavia Neto Otoch, José Pinhata Pessoa, Ana Flávia Marçal |
author_sort | Santamarina, Aline Boveto |
collection | PubMed |
description | The use of natural products and derivatives for the prevention and control of non-communicable chronic diseases, such as type-2 diabetes (T2D), obesity, and hepatic steatosis is a way to achieve homeostasis through different metabolic pathways. Thus, male C57BL/6 mice were divided into the following groups: high-fat diet (HFD) vehicle, HFD + Supplemented, HFD + Supplemented_S, and isolated compounds. The vehicle and experimental formulations were administered orally by gavage once a day over the four weeks of the diet (28 consecutive days). We evaluated the energy homeostasis, cytokines, and mitochondrial gene expression in these groups of mice. After four weeks of supplementation, only the new nutraceutical group (HFD + Supplemented) experienced reduced fasting glycemia, insulin, HOMA index, HOMA-β, dyslipidemia, ectopic fat deposition, and hepatic fibrosis levels. Additionally, the PPARγ coactivator 1 α (Pgc-1α), interleukin-6 (Il-6), and interleukin-10 (Il-10) gene expression were augmented, while hepatic steatosis decreased and liver parenchyma was recovered. The glutathione-S-transferase activity status was found to be modulated by the supplement. We discovered that the new nutraceutical was able to improve insulin resistance and hepatic steatosis mainly by regulating IL-6, IL-10, and Pgc-1α gene expression. |
format | Online Article Text |
id | pubmed-8944780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89447802022-03-25 The Symbiotic Effect of a New Nutraceutical with Yeast β-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1α, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model Santamarina, Aline Boveto Moraes, Ruan Carlos Macêdo Nehmi Filho, Victor Murata, Gilson Masahiro de Freitas, Jéssica Alves de Miranda, Danielle Araujo Cerqueira, Anderson Romério Azevedo Costa, Soraia Katia Pereira Ferreira, Ana Flávia Fernandes Britto, Luiz Roberto de Camargo, Juliana Alves Rodrigues de Oliveira, Daniela de Jesus, Flavia Neto Otoch, José Pinhata Pessoa, Ana Flávia Marçal Antioxidants (Basel) Article The use of natural products and derivatives for the prevention and control of non-communicable chronic diseases, such as type-2 diabetes (T2D), obesity, and hepatic steatosis is a way to achieve homeostasis through different metabolic pathways. Thus, male C57BL/6 mice were divided into the following groups: high-fat diet (HFD) vehicle, HFD + Supplemented, HFD + Supplemented_S, and isolated compounds. The vehicle and experimental formulations were administered orally by gavage once a day over the four weeks of the diet (28 consecutive days). We evaluated the energy homeostasis, cytokines, and mitochondrial gene expression in these groups of mice. After four weeks of supplementation, only the new nutraceutical group (HFD + Supplemented) experienced reduced fasting glycemia, insulin, HOMA index, HOMA-β, dyslipidemia, ectopic fat deposition, and hepatic fibrosis levels. Additionally, the PPARγ coactivator 1 α (Pgc-1α), interleukin-6 (Il-6), and interleukin-10 (Il-10) gene expression were augmented, while hepatic steatosis decreased and liver parenchyma was recovered. The glutathione-S-transferase activity status was found to be modulated by the supplement. We discovered that the new nutraceutical was able to improve insulin resistance and hepatic steatosis mainly by regulating IL-6, IL-10, and Pgc-1α gene expression. MDPI 2022-02-23 /pmc/articles/PMC8944780/ /pubmed/35326098 http://dx.doi.org/10.3390/antiox11030447 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Santamarina, Aline Boveto Moraes, Ruan Carlos Macêdo Nehmi Filho, Victor Murata, Gilson Masahiro de Freitas, Jéssica Alves de Miranda, Danielle Araujo Cerqueira, Anderson Romério Azevedo Costa, Soraia Katia Pereira Ferreira, Ana Flávia Fernandes Britto, Luiz Roberto de Camargo, Juliana Alves Rodrigues de Oliveira, Daniela de Jesus, Flavia Neto Otoch, José Pinhata Pessoa, Ana Flávia Marçal The Symbiotic Effect of a New Nutraceutical with Yeast β-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1α, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model |
title | The Symbiotic Effect of a New Nutraceutical with Yeast β-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1α, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model |
title_full | The Symbiotic Effect of a New Nutraceutical with Yeast β-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1α, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model |
title_fullStr | The Symbiotic Effect of a New Nutraceutical with Yeast β-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1α, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model |
title_full_unstemmed | The Symbiotic Effect of a New Nutraceutical with Yeast β-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1α, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model |
title_short | The Symbiotic Effect of a New Nutraceutical with Yeast β-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1α, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model |
title_sort | symbiotic effect of a new nutraceutical with yeast β-glucan, prebiotics, minerals, and silybum marianum (silymarin) for recovering metabolic homeostasis via pgc-1α, il-6, and il-10 gene expression in a type-2 diabetes obesity model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944780/ https://www.ncbi.nlm.nih.gov/pubmed/35326098 http://dx.doi.org/10.3390/antiox11030447 |
work_keys_str_mv | AT santamarinaalineboveto thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT moraesruancarlosmacedo thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT nehmifilhovictor thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT muratagilsonmasahiro thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT defreitasjessicaalves thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT demirandadaniellearaujo thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT cerqueiraandersonromerioazevedo thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT costasoraiakatiapereira thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT ferreiraanaflaviafernandes thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT brittoluizroberto thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT decamargojulianaalves thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT rodriguesdeoliveiradaniela thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT dejesusflavianeto thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT otochjosepinhata thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT pessoaanaflaviamarcal thesymbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT santamarinaalineboveto symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT moraesruancarlosmacedo symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT nehmifilhovictor symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT muratagilsonmasahiro symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT defreitasjessicaalves symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT demirandadaniellearaujo symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT cerqueiraandersonromerioazevedo symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT costasoraiakatiapereira symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT ferreiraanaflaviafernandes symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT brittoluizroberto symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT decamargojulianaalves symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT rodriguesdeoliveiradaniela symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT dejesusflavianeto symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT otochjosepinhata symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel AT pessoaanaflaviamarcal symbioticeffectofanewnutraceuticalwithyeastbglucanprebioticsmineralsandsilybummarianumsilymarinforrecoveringmetabolichomeostasisviapgc1ail6andil10geneexpressioninatype2diabetesobesitymodel |