Canine Spermatozoa—Predictability of Cryotolerance

SIMPLE SUMMARY: Semen freezing in dogs is a field of growing interest. The international shipment of cryoconserved semen contributes to the avoidance of long travels and long-term storage of valuable gametes. However, the collection of one semen portion on average results in one to three doses for artificial insemination, which is a poor result in comparison to the outcome in large animals. The costs for the owners are therefore rather high. In individual dogs, the semen quality of raw semen is good; however, it could be suboptimal after thawing. To avoid costly freezing of these low-quality ejaculates, markers of freezability are useful. An abundance of markers are available for large animals, but not for dogs. This review provides an overview on markers for freezability of canine semen. ABSTRACT: Markers of freezability allow the selection of ejaculates of good freezability. So far, most investigations were conducted in boars, bulls, rams and horses, with high economic interests triggering the efforts. The progress in dogs is comparably slow. A critical evaluation of the methods requires consideration of practicability, with most labs not even possessing a computer assisted sperm analyser (CASA); furthermore, small canine ejaculates mostly do not allow the use of large semen volumes. In dogs, modern markers of freezability no longer assess single membrane constituents or seminal plasma components but comprise tests of cell functionality and adaptability, energy metabolism, cluster analyses of kinetic and morphometric parameters, as well as DNA intactness. Identification of the most efficient combination of tests seems useful. At present, examination by CASA combined with cluster analysis of kinetic subgroups, JC-1 staining and COMET assay or staining with toluidine blue seem most appropriate; however, cell volumetry and other functional tests deserve better attention. A better understanding of spermatozoa energy metabolism might reveal new markers. This review focuses on the requirements and markers of freezability of canine semen, highlighting potential future candidates.