Cargando…
The Natural Cryoprotectant Honey for Fertility Cryopreservation
Honey is a mixture of 25 sugars with other bioactive substances (i.e., organic acids, enzymes, antioxidants, and vitamins) and has been known as a highly nutritious functional food. Traditionally, it has been widely used in medicinal applications to cure various diseases. The effectiveness of honey...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945096/ https://www.ncbi.nlm.nih.gov/pubmed/35324777 http://dx.doi.org/10.3390/bioengineering9030088 |
_version_ | 1784673870889877504 |
---|---|
author | Cheepa, Faryal Farooq Liu, Huilan Zhao, Gang |
author_facet | Cheepa, Faryal Farooq Liu, Huilan Zhao, Gang |
author_sort | Cheepa, Faryal Farooq |
collection | PubMed |
description | Honey is a mixture of 25 sugars with other bioactive substances (i.e., organic acids, enzymes, antioxidants, and vitamins) and has been known as a highly nutritious functional food. Traditionally, it has been widely used in medicinal applications to cure various diseases. The effectiveness of honey in different applications has been used for its antimicrobial activity, absorption of hydrops, cleansing, removing odor, assisting granulation, recovery of nutrition, and formation of tissue and epithelium, which proved that honey has dehydrating and preserving properties to make it ideal for the cryopreservation of cells and tissues. Cryopreservation is an advanced preservation technique for tissue, cells, organelles, or other biological specimen storage, performed by cooling the sample at a very low temperature. It is the most common approach to improved preserving fertility (sperm, embryos, and oocytes) in different species that may undergo various life-threatening illnesses and allows for the genetic screening of these cells to test the sample for diseases before use. However, with toxic cryoprotectant (CPA), cryopreservation of fertility has been challenging because of their particular structure and sensitivity to chilling. Honey’s unique composition, as well as its dehydrating and preserving properties, qualify it to be used as a natural cryoprotectant. The aim of this study is to emphasize the ability of honey as a natural cryoprotectant in cryopreservation. The articles for this review were searched from Google Scholar, PubMed, Science Direct, Web of Science, and Scopus, using the keywords, honey, cryopreservation, natural cryoprotectant/CPAs, extenders, and fertility. Honey, as a natural cryoprotectant in fertility cryopreservation, yielded satisfactory results, with respect to improved post-thaw quality and viability. It is now proved as a non-toxic and highly efficient natural cryoprotectant in fertility preservation because its increasing viscosity at low temperature can provide a protective barrier to cells by reducing ice formation. Furthermore, its antioxidant property plays a vital role in protecting the cells from thermal damage by reducing the reactive oxygen species (ROS). This review provides a road map for future studies to investigate the potential of honey in the cryopreservation of other cells and tissue and contribute to the scientific research, regarding this remarkable natural product. |
format | Online Article Text |
id | pubmed-8945096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89450962022-03-25 The Natural Cryoprotectant Honey for Fertility Cryopreservation Cheepa, Faryal Farooq Liu, Huilan Zhao, Gang Bioengineering (Basel) Review Honey is a mixture of 25 sugars with other bioactive substances (i.e., organic acids, enzymes, antioxidants, and vitamins) and has been known as a highly nutritious functional food. Traditionally, it has been widely used in medicinal applications to cure various diseases. The effectiveness of honey in different applications has been used for its antimicrobial activity, absorption of hydrops, cleansing, removing odor, assisting granulation, recovery of nutrition, and formation of tissue and epithelium, which proved that honey has dehydrating and preserving properties to make it ideal for the cryopreservation of cells and tissues. Cryopreservation is an advanced preservation technique for tissue, cells, organelles, or other biological specimen storage, performed by cooling the sample at a very low temperature. It is the most common approach to improved preserving fertility (sperm, embryos, and oocytes) in different species that may undergo various life-threatening illnesses and allows for the genetic screening of these cells to test the sample for diseases before use. However, with toxic cryoprotectant (CPA), cryopreservation of fertility has been challenging because of their particular structure and sensitivity to chilling. Honey’s unique composition, as well as its dehydrating and preserving properties, qualify it to be used as a natural cryoprotectant. The aim of this study is to emphasize the ability of honey as a natural cryoprotectant in cryopreservation. The articles for this review were searched from Google Scholar, PubMed, Science Direct, Web of Science, and Scopus, using the keywords, honey, cryopreservation, natural cryoprotectant/CPAs, extenders, and fertility. Honey, as a natural cryoprotectant in fertility cryopreservation, yielded satisfactory results, with respect to improved post-thaw quality and viability. It is now proved as a non-toxic and highly efficient natural cryoprotectant in fertility preservation because its increasing viscosity at low temperature can provide a protective barrier to cells by reducing ice formation. Furthermore, its antioxidant property plays a vital role in protecting the cells from thermal damage by reducing the reactive oxygen species (ROS). This review provides a road map for future studies to investigate the potential of honey in the cryopreservation of other cells and tissue and contribute to the scientific research, regarding this remarkable natural product. MDPI 2022-02-22 /pmc/articles/PMC8945096/ /pubmed/35324777 http://dx.doi.org/10.3390/bioengineering9030088 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Cheepa, Faryal Farooq Liu, Huilan Zhao, Gang The Natural Cryoprotectant Honey for Fertility Cryopreservation |
title | The Natural Cryoprotectant Honey for Fertility Cryopreservation |
title_full | The Natural Cryoprotectant Honey for Fertility Cryopreservation |
title_fullStr | The Natural Cryoprotectant Honey for Fertility Cryopreservation |
title_full_unstemmed | The Natural Cryoprotectant Honey for Fertility Cryopreservation |
title_short | The Natural Cryoprotectant Honey for Fertility Cryopreservation |
title_sort | natural cryoprotectant honey for fertility cryopreservation |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945096/ https://www.ncbi.nlm.nih.gov/pubmed/35324777 http://dx.doi.org/10.3390/bioengineering9030088 |
work_keys_str_mv | AT cheepafaryalfarooq thenaturalcryoprotectanthoneyforfertilitycryopreservation AT liuhuilan thenaturalcryoprotectanthoneyforfertilitycryopreservation AT zhaogang thenaturalcryoprotectanthoneyforfertilitycryopreservation AT cheepafaryalfarooq naturalcryoprotectanthoneyforfertilitycryopreservation AT liuhuilan naturalcryoprotectanthoneyforfertilitycryopreservation AT zhaogang naturalcryoprotectanthoneyforfertilitycryopreservation |