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Suppressing Nonspecific Binding in Biolayer Interferometry Experiments for Weak Ligand–Analyte Interactions

[Image: see text] Quantitative analysis of protein–protein interactions (PPIs) using biolayer interferometry (BLI) requires effective suppression of nonspecific binding (NSB) between analytes and biosensors. In particular, the study of weak interactions (i.e., K(D) > 1 μM) requires high concentra...

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Detalles Bibliográficos
Autores principales:	Dubrow, Alyssa, Zuniga, Bryan, Topo, Elias, Cho, Jae-Hyun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2022
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945191/ https://www.ncbi.nlm.nih.gov/pubmed/35350330 http://dx.doi.org/10.1021/acsomega.1c05659

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