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Structure of Some Green Tea Catechins and the Availability of Intracellular Copper Influence Their Ability to Cause Selective Oxidative DNA Damage in Malignant Cells

The possible roles of elevated endogenous copper levels in malignant cells are becoming increasingly understood at a greater depth. Our laboratory has previously demonstrated that tea catechins have the ability to mobilize endogenous copper and undergo a Fenton-like reaction that can selectively dam...

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Autores principales: Farhan, Mohd, Rizvi, Asim, Ahmad, Aamir, Aatif, Mohammad, Alam, Mir Waqas, Hadi, Sheikh Mumtaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945203/
https://www.ncbi.nlm.nih.gov/pubmed/35327466
http://dx.doi.org/10.3390/biomedicines10030664
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author Farhan, Mohd
Rizvi, Asim
Ahmad, Aamir
Aatif, Mohammad
Alam, Mir Waqas
Hadi, Sheikh Mumtaz
author_facet Farhan, Mohd
Rizvi, Asim
Ahmad, Aamir
Aatif, Mohammad
Alam, Mir Waqas
Hadi, Sheikh Mumtaz
author_sort Farhan, Mohd
collection PubMed
description The possible roles of elevated endogenous copper levels in malignant cells are becoming increasingly understood at a greater depth. Our laboratory has previously demonstrated that tea catechins have the ability to mobilize endogenous copper and undergo a Fenton-like reaction that can selectively damage cancer cells. In this communication, by using a diverse panel of malignant cell lines, we demonstrate that the ability of the catechin family [(−)-epigallocatechin-3-gallate (EGCG), (−)-epigallocatechin (EGC), (−)-epicatechin (EC), and (+)-catechin (C)] to induce apoptosis is dependent on their structure. We further confirm that reactive oxygen species (ROS) are the terminal effectors causing copper-mediated DNA damage. Our studies demonstrate the role of cellular copper transporters CTR1 and ATP7A in the survival dynamics of malignant cells post-EGCG exposure. The results, when considered together with our previous studies, highlight the critical role that copper dynamics and mobilization plays in cancer cells and paves the way for a better understanding of catechins as nutraceutical supplements for malignancies.
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spelling pubmed-89452032022-03-25 Structure of Some Green Tea Catechins and the Availability of Intracellular Copper Influence Their Ability to Cause Selective Oxidative DNA Damage in Malignant Cells Farhan, Mohd Rizvi, Asim Ahmad, Aamir Aatif, Mohammad Alam, Mir Waqas Hadi, Sheikh Mumtaz Biomedicines Article The possible roles of elevated endogenous copper levels in malignant cells are becoming increasingly understood at a greater depth. Our laboratory has previously demonstrated that tea catechins have the ability to mobilize endogenous copper and undergo a Fenton-like reaction that can selectively damage cancer cells. In this communication, by using a diverse panel of malignant cell lines, we demonstrate that the ability of the catechin family [(−)-epigallocatechin-3-gallate (EGCG), (−)-epigallocatechin (EGC), (−)-epicatechin (EC), and (+)-catechin (C)] to induce apoptosis is dependent on their structure. We further confirm that reactive oxygen species (ROS) are the terminal effectors causing copper-mediated DNA damage. Our studies demonstrate the role of cellular copper transporters CTR1 and ATP7A in the survival dynamics of malignant cells post-EGCG exposure. The results, when considered together with our previous studies, highlight the critical role that copper dynamics and mobilization plays in cancer cells and paves the way for a better understanding of catechins as nutraceutical supplements for malignancies. MDPI 2022-03-12 /pmc/articles/PMC8945203/ /pubmed/35327466 http://dx.doi.org/10.3390/biomedicines10030664 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Farhan, Mohd
Rizvi, Asim
Ahmad, Aamir
Aatif, Mohammad
Alam, Mir Waqas
Hadi, Sheikh Mumtaz
Structure of Some Green Tea Catechins and the Availability of Intracellular Copper Influence Their Ability to Cause Selective Oxidative DNA Damage in Malignant Cells
title Structure of Some Green Tea Catechins and the Availability of Intracellular Copper Influence Their Ability to Cause Selective Oxidative DNA Damage in Malignant Cells
title_full Structure of Some Green Tea Catechins and the Availability of Intracellular Copper Influence Their Ability to Cause Selective Oxidative DNA Damage in Malignant Cells
title_fullStr Structure of Some Green Tea Catechins and the Availability of Intracellular Copper Influence Their Ability to Cause Selective Oxidative DNA Damage in Malignant Cells
title_full_unstemmed Structure of Some Green Tea Catechins and the Availability of Intracellular Copper Influence Their Ability to Cause Selective Oxidative DNA Damage in Malignant Cells
title_short Structure of Some Green Tea Catechins and the Availability of Intracellular Copper Influence Their Ability to Cause Selective Oxidative DNA Damage in Malignant Cells
title_sort structure of some green tea catechins and the availability of intracellular copper influence their ability to cause selective oxidative dna damage in malignant cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945203/
https://www.ncbi.nlm.nih.gov/pubmed/35327466
http://dx.doi.org/10.3390/biomedicines10030664
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