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Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts

[Image: see text] Genetic engineering of economically important traits in plants is an effective way to improve global welfare. However, introducing foreign DNA molecules into plant genomes to create genetically engineered plants not only requires a lengthy testing period and high developmental cost...

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Autores principales: Thagun, Chonprakun, Horii, Yoko, Mori, Maai, Fujita, Seiya, Ohtani, Misato, Tsuchiya, Kousuke, Kodama, Yutaka, Odahara, Masaki, Numata, Keiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945396/
https://www.ncbi.nlm.nih.gov/pubmed/35195009
http://dx.doi.org/10.1021/acsnano.1c07723
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author Thagun, Chonprakun
Horii, Yoko
Mori, Maai
Fujita, Seiya
Ohtani, Misato
Tsuchiya, Kousuke
Kodama, Yutaka
Odahara, Masaki
Numata, Keiji
author_facet Thagun, Chonprakun
Horii, Yoko
Mori, Maai
Fujita, Seiya
Ohtani, Misato
Tsuchiya, Kousuke
Kodama, Yutaka
Odahara, Masaki
Numata, Keiji
author_sort Thagun, Chonprakun
collection PubMed
description [Image: see text] Genetic engineering of economically important traits in plants is an effective way to improve global welfare. However, introducing foreign DNA molecules into plant genomes to create genetically engineered plants not only requires a lengthy testing period and high developmental costs but also is not well-accepted by the public due to safety concerns about its effects on human and animal health and the environment. Here, we present a high-throughput nucleic acids delivery platform for plants using peptide nanocarriers applied to the leaf surface by spraying. The translocation of sub-micrometer-scale nucleic acid/peptide complexes upon spraying varied depending on the physicochemical characteristics of the peptides and was controlled by a stomata-dependent-uptake mechanism in plant cells. We observed efficient delivery of DNA molecules into plants using cell-penetrating peptide (CPP)-based foliar spraying. Moreover, using foliar spraying, we successfully performed gene silencing by introducing small interfering RNA molecules in plant nuclei via siRNA-CPP complexes and, more importantly, in chloroplasts via our CPP/chloroplast-targeting peptide-mediated delivery system. This technology enables effective nontransgenic engineering of economically important plant traits in agricultural systems.
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spelling pubmed-89453962022-03-28 Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts Thagun, Chonprakun Horii, Yoko Mori, Maai Fujita, Seiya Ohtani, Misato Tsuchiya, Kousuke Kodama, Yutaka Odahara, Masaki Numata, Keiji ACS Nano [Image: see text] Genetic engineering of economically important traits in plants is an effective way to improve global welfare. However, introducing foreign DNA molecules into plant genomes to create genetically engineered plants not only requires a lengthy testing period and high developmental costs but also is not well-accepted by the public due to safety concerns about its effects on human and animal health and the environment. Here, we present a high-throughput nucleic acids delivery platform for plants using peptide nanocarriers applied to the leaf surface by spraying. The translocation of sub-micrometer-scale nucleic acid/peptide complexes upon spraying varied depending on the physicochemical characteristics of the peptides and was controlled by a stomata-dependent-uptake mechanism in plant cells. We observed efficient delivery of DNA molecules into plants using cell-penetrating peptide (CPP)-based foliar spraying. Moreover, using foliar spraying, we successfully performed gene silencing by introducing small interfering RNA molecules in plant nuclei via siRNA-CPP complexes and, more importantly, in chloroplasts via our CPP/chloroplast-targeting peptide-mediated delivery system. This technology enables effective nontransgenic engineering of economically important plant traits in agricultural systems. American Chemical Society 2022-02-23 2022-03-22 /pmc/articles/PMC8945396/ /pubmed/35195009 http://dx.doi.org/10.1021/acsnano.1c07723 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Thagun, Chonprakun
Horii, Yoko
Mori, Maai
Fujita, Seiya
Ohtani, Misato
Tsuchiya, Kousuke
Kodama, Yutaka
Odahara, Masaki
Numata, Keiji
Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts
title Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts
title_full Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts
title_fullStr Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts
title_full_unstemmed Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts
title_short Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts
title_sort non-transgenic gene modulation via spray delivery of nucleic acid/peptide complexes into plant nuclei and chloroplasts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945396/
https://www.ncbi.nlm.nih.gov/pubmed/35195009
http://dx.doi.org/10.1021/acsnano.1c07723
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