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The Role of Membrane Lipids in Light-Activation of Drosophila TRP Channels

Transient Receptor Potential (TRP) channels constitute a large superfamily of polymodal channel proteins with diverse roles in many physiological and sensory systems that function both as ionotropic and metabotropic receptors. From the early days of TRP channel discovery, membrane lipids were sugges...

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Autores principales: Gutorov, Rita, Katz, Ben, Rhodes-Mordov, Elisheva, Zaguri, Rachel, Brandwine-Shemmer, Tal, Minke, Baruch
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945425/
https://www.ncbi.nlm.nih.gov/pubmed/35327573
http://dx.doi.org/10.3390/biom12030382
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author Gutorov, Rita
Katz, Ben
Rhodes-Mordov, Elisheva
Zaguri, Rachel
Brandwine-Shemmer, Tal
Minke, Baruch
author_facet Gutorov, Rita
Katz, Ben
Rhodes-Mordov, Elisheva
Zaguri, Rachel
Brandwine-Shemmer, Tal
Minke, Baruch
author_sort Gutorov, Rita
collection PubMed
description Transient Receptor Potential (TRP) channels constitute a large superfamily of polymodal channel proteins with diverse roles in many physiological and sensory systems that function both as ionotropic and metabotropic receptors. From the early days of TRP channel discovery, membrane lipids were suggested to play a fundamental role in channel activation and regulation. A prominent example is the Drosophila TRP and TRP-like (TRPL) channels, which are predominantly expressed in the visual system of Drosophila. Light activation of the TRP and TRPL channels, the founding members of the TRP channel superfamily, requires activation of phospholipase Cβ (PLC), which hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP(2)) into Diacylglycerol (DAG) and Inositol 1, 4,5-trisphosphate (IP(3)). However, the events required for channel gating downstream of PLC activation are still under debate and led to several hypotheses regarding the mechanisms by which lipids gate the channels. Despite many efforts, compelling evidence of the involvement of DAG accumulation, PIP(2) depletion or IP(3)-mediated Ca(2+) release in light activation of the TRP/TRPL channels are still lacking. Exogeneous application of poly unsaturated fatty acids (PUFAs), a product of DAG hydrolysis was demonstrated as an efficient way to activate the Drosophila TRP/TRPL channels. However, compelling evidence for the involvement of PUFAs in physiological light-activation of the TRP/TRPL channels is still lacking. Light-induced mechanical force generation was measured in photoreceptor cells prior to channel opening. This mechanical force depends on PLC activity, suggesting that the enzymatic activity of PLC converting PIP(2) into DAG generates membrane tension, leading to mechanical gating of the channels. In this review, we will present the roles of membrane lipids in light activation of Drosophila TRP channels and present the many advantages of this model system in the exploration of TRP channel activation under physiological conditions.
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spelling pubmed-89454252022-03-25 The Role of Membrane Lipids in Light-Activation of Drosophila TRP Channels Gutorov, Rita Katz, Ben Rhodes-Mordov, Elisheva Zaguri, Rachel Brandwine-Shemmer, Tal Minke, Baruch Biomolecules Review Transient Receptor Potential (TRP) channels constitute a large superfamily of polymodal channel proteins with diverse roles in many physiological and sensory systems that function both as ionotropic and metabotropic receptors. From the early days of TRP channel discovery, membrane lipids were suggested to play a fundamental role in channel activation and regulation. A prominent example is the Drosophila TRP and TRP-like (TRPL) channels, which are predominantly expressed in the visual system of Drosophila. Light activation of the TRP and TRPL channels, the founding members of the TRP channel superfamily, requires activation of phospholipase Cβ (PLC), which hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP(2)) into Diacylglycerol (DAG) and Inositol 1, 4,5-trisphosphate (IP(3)). However, the events required for channel gating downstream of PLC activation are still under debate and led to several hypotheses regarding the mechanisms by which lipids gate the channels. Despite many efforts, compelling evidence of the involvement of DAG accumulation, PIP(2) depletion or IP(3)-mediated Ca(2+) release in light activation of the TRP/TRPL channels are still lacking. Exogeneous application of poly unsaturated fatty acids (PUFAs), a product of DAG hydrolysis was demonstrated as an efficient way to activate the Drosophila TRP/TRPL channels. However, compelling evidence for the involvement of PUFAs in physiological light-activation of the TRP/TRPL channels is still lacking. Light-induced mechanical force generation was measured in photoreceptor cells prior to channel opening. This mechanical force depends on PLC activity, suggesting that the enzymatic activity of PLC converting PIP(2) into DAG generates membrane tension, leading to mechanical gating of the channels. In this review, we will present the roles of membrane lipids in light activation of Drosophila TRP channels and present the many advantages of this model system in the exploration of TRP channel activation under physiological conditions. MDPI 2022-02-28 /pmc/articles/PMC8945425/ /pubmed/35327573 http://dx.doi.org/10.3390/biom12030382 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gutorov, Rita
Katz, Ben
Rhodes-Mordov, Elisheva
Zaguri, Rachel
Brandwine-Shemmer, Tal
Minke, Baruch
The Role of Membrane Lipids in Light-Activation of Drosophila TRP Channels
title The Role of Membrane Lipids in Light-Activation of Drosophila TRP Channels
title_full The Role of Membrane Lipids in Light-Activation of Drosophila TRP Channels
title_fullStr The Role of Membrane Lipids in Light-Activation of Drosophila TRP Channels
title_full_unstemmed The Role of Membrane Lipids in Light-Activation of Drosophila TRP Channels
title_short The Role of Membrane Lipids in Light-Activation of Drosophila TRP Channels
title_sort role of membrane lipids in light-activation of drosophila trp channels
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945425/
https://www.ncbi.nlm.nih.gov/pubmed/35327573
http://dx.doi.org/10.3390/biom12030382
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