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TLR4 Expression in Ex-Lichenoid Lesions—Oral Squamous Cell Carcinomas and Its Surrounding Epithelium: The Role of Tumor Inflammatory Microenvironment

Toll-like receptors (TLRs) regulate innate and adaptive immune responses. Moreover, TLRs can induce a pro-survival and pro-proliferation response in tumor cells. This study aims to investigate the expression of TLR4 in the epithelium surrounding oral squamous cell carcinomas (OSCC) in relation to it...

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Autores principales: Visioli, Fernanda, Nunes, Julia Silveira, Pedicillo, Maria Carmela, Leonardi, Rosalia, Santoro, Angela, Zannoni, Gian Franco, Aquino, Gabriella, Cerrone, Margherita, Cantile, Monica, Losito, Nunzia Simona, Rodolico, Vito, Campisi, Giuseppina, Colella, Giuseppe, De Stefano, Ilenia Sara, Ramunno, Maria Antonietta, Pizzulli, Cristina, Visconti, Marco, Lo Muzio, Lorenzo, Pannone, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945442/
https://www.ncbi.nlm.nih.gov/pubmed/35327577
http://dx.doi.org/10.3390/biom12030385
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author Visioli, Fernanda
Nunes, Julia Silveira
Pedicillo, Maria Carmela
Leonardi, Rosalia
Santoro, Angela
Zannoni, Gian Franco
Aquino, Gabriella
Cerrone, Margherita
Cantile, Monica
Losito, Nunzia Simona
Rodolico, Vito
Campisi, Giuseppina
Colella, Giuseppe
De Stefano, Ilenia Sara
Ramunno, Maria Antonietta
Pizzulli, Cristina
Visconti, Marco
Lo Muzio, Lorenzo
Pannone, Giuseppe
author_facet Visioli, Fernanda
Nunes, Julia Silveira
Pedicillo, Maria Carmela
Leonardi, Rosalia
Santoro, Angela
Zannoni, Gian Franco
Aquino, Gabriella
Cerrone, Margherita
Cantile, Monica
Losito, Nunzia Simona
Rodolico, Vito
Campisi, Giuseppina
Colella, Giuseppe
De Stefano, Ilenia Sara
Ramunno, Maria Antonietta
Pizzulli, Cristina
Visconti, Marco
Lo Muzio, Lorenzo
Pannone, Giuseppe
author_sort Visioli, Fernanda
collection PubMed
description Toll-like receptors (TLRs) regulate innate and adaptive immune responses. Moreover, TLRs can induce a pro-survival and pro-proliferation response in tumor cells. This study aims to investigate the expression of TLR4 in the epithelium surrounding oral squamous cell carcinomas (OSCC) in relation to its inflammatory microenvironment. This study included 150 human samples: 30 normal oral control (NOC), 38 non-lichenoid epithelium surrounding OSCC (NLE-OSCC), 28 lichenoid epithelium surrounding OSCC (LE-OSCC), 30 OSCC ex-non oral lichenoid lesion (OSCC Ex-NOLL), and 24 OSCC ex-oral lichenoid lesion (OSCC Ex-OLL). TLR4 expression was investigated by immunohistochemistry and the percentage of positive cells was quantified. In addition, a semiquantitative analysis of staining intensity was performed. Immunohistochemical analysis revealed that TLR4 is strongly upregulated in LE-OSCC as compared to normal control epithelium and NLE-OSCC. TLR4 expression was associated with the inflammatory environment, since the percentage of positive cells increases from NOC and NLE-OSCC to LE-OSCC, reaching the highest value in OSCC Ex–OLL. TLR4 was detected in the basal third of the epithelium in NLE-OSCC, while in LE-OSCC, TLR4 expression reached the intermediate layer. These results demonstrated that an inflammatory microenvironment can upregulate TLR4, which may boost tumor development.
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spelling pubmed-89454422022-03-25 TLR4 Expression in Ex-Lichenoid Lesions—Oral Squamous Cell Carcinomas and Its Surrounding Epithelium: The Role of Tumor Inflammatory Microenvironment Visioli, Fernanda Nunes, Julia Silveira Pedicillo, Maria Carmela Leonardi, Rosalia Santoro, Angela Zannoni, Gian Franco Aquino, Gabriella Cerrone, Margherita Cantile, Monica Losito, Nunzia Simona Rodolico, Vito Campisi, Giuseppina Colella, Giuseppe De Stefano, Ilenia Sara Ramunno, Maria Antonietta Pizzulli, Cristina Visconti, Marco Lo Muzio, Lorenzo Pannone, Giuseppe Biomolecules Article Toll-like receptors (TLRs) regulate innate and adaptive immune responses. Moreover, TLRs can induce a pro-survival and pro-proliferation response in tumor cells. This study aims to investigate the expression of TLR4 in the epithelium surrounding oral squamous cell carcinomas (OSCC) in relation to its inflammatory microenvironment. This study included 150 human samples: 30 normal oral control (NOC), 38 non-lichenoid epithelium surrounding OSCC (NLE-OSCC), 28 lichenoid epithelium surrounding OSCC (LE-OSCC), 30 OSCC ex-non oral lichenoid lesion (OSCC Ex-NOLL), and 24 OSCC ex-oral lichenoid lesion (OSCC Ex-OLL). TLR4 expression was investigated by immunohistochemistry and the percentage of positive cells was quantified. In addition, a semiquantitative analysis of staining intensity was performed. Immunohistochemical analysis revealed that TLR4 is strongly upregulated in LE-OSCC as compared to normal control epithelium and NLE-OSCC. TLR4 expression was associated with the inflammatory environment, since the percentage of positive cells increases from NOC and NLE-OSCC to LE-OSCC, reaching the highest value in OSCC Ex–OLL. TLR4 was detected in the basal third of the epithelium in NLE-OSCC, while in LE-OSCC, TLR4 expression reached the intermediate layer. These results demonstrated that an inflammatory microenvironment can upregulate TLR4, which may boost tumor development. MDPI 2022-02-28 /pmc/articles/PMC8945442/ /pubmed/35327577 http://dx.doi.org/10.3390/biom12030385 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Visioli, Fernanda
Nunes, Julia Silveira
Pedicillo, Maria Carmela
Leonardi, Rosalia
Santoro, Angela
Zannoni, Gian Franco
Aquino, Gabriella
Cerrone, Margherita
Cantile, Monica
Losito, Nunzia Simona
Rodolico, Vito
Campisi, Giuseppina
Colella, Giuseppe
De Stefano, Ilenia Sara
Ramunno, Maria Antonietta
Pizzulli, Cristina
Visconti, Marco
Lo Muzio, Lorenzo
Pannone, Giuseppe
TLR4 Expression in Ex-Lichenoid Lesions—Oral Squamous Cell Carcinomas and Its Surrounding Epithelium: The Role of Tumor Inflammatory Microenvironment
title TLR4 Expression in Ex-Lichenoid Lesions—Oral Squamous Cell Carcinomas and Its Surrounding Epithelium: The Role of Tumor Inflammatory Microenvironment
title_full TLR4 Expression in Ex-Lichenoid Lesions—Oral Squamous Cell Carcinomas and Its Surrounding Epithelium: The Role of Tumor Inflammatory Microenvironment
title_fullStr TLR4 Expression in Ex-Lichenoid Lesions—Oral Squamous Cell Carcinomas and Its Surrounding Epithelium: The Role of Tumor Inflammatory Microenvironment
title_full_unstemmed TLR4 Expression in Ex-Lichenoid Lesions—Oral Squamous Cell Carcinomas and Its Surrounding Epithelium: The Role of Tumor Inflammatory Microenvironment
title_short TLR4 Expression in Ex-Lichenoid Lesions—Oral Squamous Cell Carcinomas and Its Surrounding Epithelium: The Role of Tumor Inflammatory Microenvironment
title_sort tlr4 expression in ex-lichenoid lesions—oral squamous cell carcinomas and its surrounding epithelium: the role of tumor inflammatory microenvironment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945442/
https://www.ncbi.nlm.nih.gov/pubmed/35327577
http://dx.doi.org/10.3390/biom12030385
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