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Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis

Gelsolin (GSN) is an actin-binding protein involved in cell formation, metabolism and wound closure processes. Since this protein is known to play a role in arthritis, here we investigate how the synovial membrane with its specific synoviocytes contributes to the expression of GSN and how the amount...

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Autores principales: Feldt, Jessica, Schicht, Martin, Welss, Jessica, Gelse, Kolja, Sesselmann, Stefan, Tsokos, Michael, Socher, Eileen, Garreis, Fabian, Müller, Thomas, Paulsen, Friedrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945596/
https://www.ncbi.nlm.nih.gov/pubmed/35327525
http://dx.doi.org/10.3390/biomedicines10030723
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author Feldt, Jessica
Schicht, Martin
Welss, Jessica
Gelse, Kolja
Sesselmann, Stefan
Tsokos, Michael
Socher, Eileen
Garreis, Fabian
Müller, Thomas
Paulsen, Friedrich
author_facet Feldt, Jessica
Schicht, Martin
Welss, Jessica
Gelse, Kolja
Sesselmann, Stefan
Tsokos, Michael
Socher, Eileen
Garreis, Fabian
Müller, Thomas
Paulsen, Friedrich
author_sort Feldt, Jessica
collection PubMed
description Gelsolin (GSN) is an actin-binding protein involved in cell formation, metabolism and wound closure processes. Since this protein is known to play a role in arthritis, here we investigate how the synovial membrane with its specific synoviocytes contributes to the expression of GSN and how the amount of GSN expressed is modulated by different types of arthritis. Synovial membranes from adult healthy subjects and patients with rheumatoid arthritis (RA) and osteoarthritis (OA) are analyzed by immunofluorescence, Western blot and ELISA. Macrophage-like synoviocytes (MLS) and fibroblast-like synoviocytes (FLS) were isolated, cultured and analyzed for their potential to produce and secrete GSN. In addition, the GSN concentrations in the synovial fluid of various forms of arthritis are determined by ELISA. GSN is produced by the healthy and arthritic synovial membranes. Both forms of synoviocytes (MLS and FLS) release GSN. The results show that there is a significant reduction in GSN in the synovial fluid in adult patients with OA. This reduction is also detectable in adult patients with RA but is not as evident. In juvenile arthritis, there is a slight increase in GSN concentration in the synovial fluid. This study shows that primary MLS and FLS express GSN and that these cells, in addition to articular chondrocytes, contribute to GSN levels in synovial fluid. Furthermore, GSN concentrations are modulated in different types of arthritis. Further studies are needed to fully understand how GSN is involved in joint homeostasis.
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spelling pubmed-89455962022-03-25 Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis Feldt, Jessica Schicht, Martin Welss, Jessica Gelse, Kolja Sesselmann, Stefan Tsokos, Michael Socher, Eileen Garreis, Fabian Müller, Thomas Paulsen, Friedrich Biomedicines Article Gelsolin (GSN) is an actin-binding protein involved in cell formation, metabolism and wound closure processes. Since this protein is known to play a role in arthritis, here we investigate how the synovial membrane with its specific synoviocytes contributes to the expression of GSN and how the amount of GSN expressed is modulated by different types of arthritis. Synovial membranes from adult healthy subjects and patients with rheumatoid arthritis (RA) and osteoarthritis (OA) are analyzed by immunofluorescence, Western blot and ELISA. Macrophage-like synoviocytes (MLS) and fibroblast-like synoviocytes (FLS) were isolated, cultured and analyzed for their potential to produce and secrete GSN. In addition, the GSN concentrations in the synovial fluid of various forms of arthritis are determined by ELISA. GSN is produced by the healthy and arthritic synovial membranes. Both forms of synoviocytes (MLS and FLS) release GSN. The results show that there is a significant reduction in GSN in the synovial fluid in adult patients with OA. This reduction is also detectable in adult patients with RA but is not as evident. In juvenile arthritis, there is a slight increase in GSN concentration in the synovial fluid. This study shows that primary MLS and FLS express GSN and that these cells, in addition to articular chondrocytes, contribute to GSN levels in synovial fluid. Furthermore, GSN concentrations are modulated in different types of arthritis. Further studies are needed to fully understand how GSN is involved in joint homeostasis. MDPI 2022-03-21 /pmc/articles/PMC8945596/ /pubmed/35327525 http://dx.doi.org/10.3390/biomedicines10030723 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feldt, Jessica
Schicht, Martin
Welss, Jessica
Gelse, Kolja
Sesselmann, Stefan
Tsokos, Michael
Socher, Eileen
Garreis, Fabian
Müller, Thomas
Paulsen, Friedrich
Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis
title Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis
title_full Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis
title_fullStr Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis
title_full_unstemmed Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis
title_short Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis
title_sort production and secretion of gelsolin by both human macrophage- and fibroblast-like synoviocytes and gsn modulation in the synovial fluid of patients with various forms of arthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945596/
https://www.ncbi.nlm.nih.gov/pubmed/35327525
http://dx.doi.org/10.3390/biomedicines10030723
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